Batch production method of medicinal dioscorea zingiberensis

Abstract

The invention discloses an effective and low-energy-consumption rapid propagation method of dioscorea zingiberensis. The method comprises steps as follows: (1) stems of an excellent medicinal dioscorea zingiberensis plant are selected as explants, the explants are disinfected and then cultured in an agar solid culture medium containing MS, 6-BA (0.01-0.05 mg / L), paclobutrazol (3.0-10.0 mg / L) and chlormequat chloride (3.0-10.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled, and a large quantity of small bud clusters are induced; (2) the cluster buds obtained in the step (1) are cut off and divided into clusters, each cluster comprises five buds, an agar solid culture medium containing MS, NAA (0.02-0.6 mg / L), IAA (0.02-0.6 mg / L), paclobutrazol (0.5-5.0 mg / L) and chlormequat chloride (0.5-5.0 mg / L) and having the content of components excluding inositol unchanged and the content of inositol doubled is inoculated with the buds for proliferation and rooting. The method is good in cultivation effect, high in proliferation coefficient, good in stability and low in production cost and is a rapid propagation method for obtaining a large quantity of test-tube plantlets within the shortest time.

Description

technical field [0001] The invention belongs to the technical field of tissue culture of medicinal Dioscorea scutellaria, and in particular relates to an efficient batch production method of medicinal Dioscorea scutellaria. Background technique [0002] Dioscorea plants (Dioscorea) have important medicinal value, and their rhizogenin is an important starting material for the synthesis of contraceptives and steroid hormones, and plays an important role in the pharmaceutical industry. In the "List of Rare and Endangered Plants in China", it is listed as a national second-class endangered species and is protected. The large-scale tissue culture, rapid propagation and standardized planting of salvia hispanica L by plant cell engineering technology and methods play an important role in promoting the development of the pharmaceutical industry. In 2014, a batch production method of one-step seedling growth of medicinal Dioscorea scutellaria was provided (authorization number ZL201...

AI Technical Summary

Problems solved by technology

In 2014, a batch production method of one-step seedling growth of medicinal Dioscorea scutellaria was provided (authorization number ZL201410288573.9), which can simultaneously induce callus, buds, and adventitious roots, and simplify multiplication and rooting in one step. The seedlings need to go through the stage of callus culture, which increases the mutation rate

In 2015, a method for rooting of aseptic short shoots of medicinal Dioscorea scutellaria tissue culture seedlings was provided (application number 201510319287.9). The height of excellent characteristics is consistent, but the number of effective seedlings is few (at most 18 strains / bottle) in the single bottle test-tube plantlet of this inventive method, and test-tube plantlet is a single plant, and clustering effect is poor, and then causes test-tube plantlet transplanting survival rate to be low, single Plant cost is high

Images