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Efficient induction medium formula of sorghum anther

A technology for inducing culture medium and sorghum flowers, which is applied in the field of agricultural science and can solve problems such as imperfect methods and culture medium

Inactive Publication Date: 2015-06-10
吕娜
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the anther culture of wheat, rice, corn and woody plants has been successful, but the method and medium for inducing haploid in sorghum anther culture are still not perfect

Method used

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  • Efficient induction medium formula of sorghum anther
  • Efficient induction medium formula of sorghum anther
  • Efficient induction medium formula of sorghum anther

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Prepare the culture medium with the following formula:

[0014] KNO 3 1650mg / L, NH 4 NO 3 1000mg / L, KH 2 PO 4 250mg / L, CaCl 2 ?2H 2 O 300mg / L, MgSO 4 130mg / L, Na 2 -EDTA 37.5mg / L, FeSO 4 ?7H 2 O 28mg / L, MnSO 4 ?H 2 O 11mg / L, ZnSO 4 ?7H 2 O 6.0mg / L, H 3 BO 3 4.0mg / L, KI 1.0mg / L, CuSO 4 ?5H 2 O 0.04mg / L, CoCl?6H 2 O 0.04mg / L, NaMoO 4 ?2H 2 O 0.1mg / L, ethyl methanesulfonate 0.6mg / L, glycine 2.0mg / L, inositol 50mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 0.5mg / L, D-arabinosyl-2-deoxyhexose 0.25g / L, sucrose 35g / L, plant gel 5.0g / L;

[0015] 2,4-D 2.5mg / L, kinetin 1.5mg / L, complex nucleic acid 0.8mg / L, phytosulfokinetin PSK-α 40pmol / L, insulin 5.0mg / L, hydrolyzed protein 0.3g / L, PH 5.9.

[0016] Sorghum cultivars Guandongqing and Yuanyuanhong were selected as donors for anther culture, and the panicles on the main stem were collected. The microspores in the anthers were in the late mononucleate stage. After surface disinfection, they were ...

Embodiment 2

[0018] Prepare the medium with the following formula (the preferred concentration of ethyl methanesulfonate added):

[0019] KNO 3 1650mg / L, NH 4 NO 3 1000mg / L, KH 2 PO 4 250mg / L, CaCl 2 ?2H 2 O 300mg / L, MgSO 4 130mg / L, Na 2 -EDTA 37.5mg / L, FeSO 4 ?7H 2 O 28mg / L, MnSO 4 ?H 2 O 11mg / L, ZnSO 4 ?7H 2 O 6.0mg / L, H 3 BO 3 4.0mg / L, KI 1.0mg / L, CuSO 4 ?5H 2 O 0.04mg / L, CoCl?6H 2 O 0.04mg / L, NaMoO 4 ?2H 2 O 0.1mg / L, glycine 2.0mg / L, inositol 50mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 0.5mg / L, D-arabinosyl-2-deoxyhexidine Sugar 0.25g / L, sucrose 35g / L, plant gel 5.0g / L;

[0020] 2,4-D 2.5mg / L, kinetin 1.5mg / L, complex nucleic acid 0.8mg / L, phytosulfokinetin PSK-α 40pmol / L, insulin 5.0mg / L, hydrolyzed protein 0.3g / L, PH 5.9.

[0021] The concentration of ethyl methanesulfonate is set in the following 8 types: 0; 0.1 mg / L; 0.2 mg / L; 0.3 mg / L; 0.4mg / L; 0.6mg / L; 0.7mg / L; .

[0022] The two sorghum varieties were also selected as anther donors, the ope...

Embodiment 3

[0024] Prepare the culture medium with the following formula (preferred coagulant):

[0025] KNO 3 1650mg / L, NH 4 NO 3 1000mg / L, KH 2 PO 4 250mg / L, CaCl 2 ?2H 2 O 300mg / L, MgSO 4 130mg / L, Na 2 -EDTA 37.5mg / L, FeSO 4 ?7H 2 O 28mg / L, MnSO 4 ?H 2 O 11mg / L, ZnSO 4 ?7H 2 O 6.0mg / L, H 3 BO 3 4.0mg / L, KI 1.0mg / L, CuSO 4 ?5H 2 O 0.04mg / L, CoCl?6H 2 O 0.04mg / L, NaMoO 4 ?2H 2 O 0.1mg / L, ethyl methanesulfonate 0.6mg / L, glycine 2.0mg / L, inositol 50mg / L, vitamin B1 0.5mg / L, vitamin B6 0.5mg / L, niacin 0.5mg / L, D-arabinosyl-2-deoxyhexose 0.25g / L, sucrose 35g / L;

[0026] 2,4-D 2.5mg / L, kinetin 1.5mg / L, complex nucleic acid 0.8mg / L, phytosulfokinetin PSK-α 40pmol / L, insulin 5.0mg / L, hydrolyzed protein 0.3g / L, PH 5.9.

[0027] The coagulant is set to the following 2 types: plant gel 2.5g / L, agar 3.5g; agar 7g.

[0028] The two sorghum varieties were also selected as anther donors, the operation method and culture method were the same as in Example 1, and the callus ...

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Abstract

The invention provides an efficient induction medium of sorghum anther. The formula of the medium is composed of a certain content of KNO3, NH4NO3, KH2PO4, CaC12 2H2O, MgSO4, Na2-EDTA, FeSO4 7H2O, MnSO4 H2O, ZnSO4 7H2O, H3BO3, KI, CuSO4 5H2O, CoC1 6H2O, NaMoO4 2H2O, ethylmethane sulfonate, glycine, inositol, vitamin B1, vitamin B6, nicotinic acid, D-arabinose base-2-deoxidation hexose, cane sugar, Phytagel, 2,4-D, kinetin, compound phthalein nucleic acid, plant sulfuration kinetin PSK-alpha, insulin and protein hydrolysate. The medium has the advantages of being capable of remarkably improving the callus rate of the sorghum anther and substantially improving the cultivation efficiency of the sorghum anther.

Description

technical field [0001] The invention relates to a high-efficiency induction medium formula for sorghum anthers, in particular to an induction medium formula for significantly improving the healing rate of sorghum anthers, and belongs to the field of agricultural science and technology. Background technique [0002] Sorghum is an annual herbaceous plant belonging to the grass family Sorghum, and it is one of the earliest cereal crops planted in my country. Sorghum occupies an important position in the world's grain production, with an annual planting area of ​​about 45 million hectares in 47 countries on 6 continents, making it the fifth largest crop in the world. Sorghum has multiple uses such as food, feed, brewing, energy and processing. In my country, sorghum is mainly used for wine making. However, with the changes in animal husbandry, energy industry, processing industry and dietary structure, feed, forage Sorghum with special uses such as sorghum, energy sweet sorghum,...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 吕娜
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