A normally-off HEMT device with a honeycomb groove barrier layer structure under a field plate and a preparation method thereof, belonging to the field of semiconductor devices. Technical solution: sequentially grow a buffer layer, an i-GaN layer, an insertion layer, a barrier layer, and a gate dielectric layer on a semiconductor substrate, where a number of honeycomb grooves are etched in a local area of the barrier layer, and the i-GaN layer One side is etched into a step layer, a source electrode is set on the step layer, a drain electrode is set on the other side of the i-GaN layer, a gate dielectric layer is set above the barrier layer, and one end of the gate dielectric layer is connected to the The drain electrode is contacted and connected, the other end covers and grows into the honeycomb groove, and extends to the source electrode, and a gate electrode is arranged on the gate dielectric layer and in the corresponding area of the honeycomb groove, and the gate electrode extending toward the source electrode. Beneficial effects: the invention can realize stable, large threshold voltage and normally-off operation with low on-resistance of the HEMT device, while effectively reducing the off-state leakage of the device and improving the breakdown voltage of the device.