The invention relates to a method for separating and purifying flavone from wormwood through imitating
moving bed chromatography. It comprises following steps: measuring 0.7- 1.0 kg dried and disintegrated wormwood, adding 10- 15kg 80%
ethanol at constant temperature for water bath refluxing and extracting, filtering, combining filterate; extracting for two times, washing residuel, combining with washing liquid,
defatting with ligarine, depressing and condensing and getting coarse wormwood ligarine extract; preparing feed liquid with said coarse extract and feeding it to imitating
moving bed for separation and purification; dissloving said coarse extract with
methanol, filtering with organic filtering membrane of 0.45 mu m and getting feed liquid for imitating
moving bed with concentration being 100- 300 mg / ml; the washing liquid is mixture of
methanol and water, the volumetric ratio between
methanol and water is 40: 60; condensing extract liquid to saturation, reducing temperature gradually to 4- 10 Deg. C below zero, crystallizing for 5- 6 hours, filtering, washing
crystal for two times,
vacuum drying and getting pure wormwood flavone. The invention is characterized by increased
production automation degree, improved working efficiency and production working condition.