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Method for separating and purifying flavone from artemisia selengensis by simulation moving bed chromatography

A simulated moving bed, separation and purification technology, applied in the field of simulated moving bed chromatography to separate and purify flavonoids from Artemisia annua, can solve problems such as no public reports, and achieve the effects of high yield, improved production environment, and improved automation level

Active Publication Date: 2007-12-12
JIANGSU HANBON SCI & TECH CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is still no public report on the method for the separation and purification of flavonoids in Artemisia annua using simulated moving bed chromatography

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0013] Example 1: Separation and purification of flavonoids from Artemisia annua by simulated moving bed chromatography in Jiangsu Hanbang Technology Co., Ltd. according to the following steps:

[0014] a. Weigh 0.7kg of dry and pulverized Artemisia annua, add 10kg of 80% ethanol at a constant temperature of 85°C to carry out water bath reflux extraction, filter and combine the filtrates;

[0015] b. Repeat the extraction twice, wash the residue, combine the washing liquid, degrease with petroleum ether, concentrate under reduced pressure, and obtain the crude extract of Artemisia flavonoids;

[0016] c. The crude extract of artemisia flavonoids is made into a sample solution and sent to a simulated moving bed for separation and purification; the crude extract of artemisia flavones is dissolved in methanol, and filtered through a 0.45 μm organic filter membrane to obtain a simulated moving bed feed solution with a concentration of 100 mg / ml ; The eluent is a mixture of methano...

example 2

[0022] According to the following steps, flavonoids were separated and purified from Artemisia annua by simulated moving bed chromatography of Jiangsu Hanbang Technology Co., Ltd.:

[0023] a. Weigh 0.9kg of dry and pulverized Artemisia annua, add 12kg of 80% ethanol at a constant temperature of 90°C to carry out water bath reflux extraction, filter and combine the filtrates;

[0024] b. Repeat the extraction twice, wash the residue, combine the washing liquid, degrease with petroleum ether, concentrate under reduced pressure, and obtain the crude extract of Artemisia flavonoids;

[0025] c. The crude extract of artemisia flavonoids is made into a sample solution and sent to a simulated moving bed for separation and purification; the crude extract of artemisia flavonoids is dissolved in methanol, and filtered through a 0.45 μm organic filter membrane to obtain a simulated moving bed feed solution with a concentration of 200 mg / ml ; The eluent is a mixture of methanol and water...

example 3

[0031] According to the following steps, flavonoids were separated and purified from Artemisia annua by simulated moving bed chromatography of Jiangsu Hanbang Technology Co., Ltd.:

[0032] a. Weigh 1 kg of dry and pulverized Artemisia annua, add 15 kg of 80% ethanol at a constant temperature of 95° C. to carry out reflux extraction in a water bath, filter and combine the filtrates;

[0033] b. Repeat the extraction twice, wash the residue, combine the washing liquid, degrease with petroleum ether, concentrate under reduced pressure, and obtain the crude extract of Artemisia flavonoids;

[0034] c. The crude extract of Artemisia flavonoids is made into a sample solution and sent to a simulated moving bed for separation and purification; the crude extract of Artemisia flavones is dissolved in methanol, filtered through a 0.45 μm organic filter membrane to obtain a simulated moving bed with a concentration of 100-300 mg / ml. feed liquid; eluent is the mixture of methanol and wate...

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Abstract

The invention relates to a method for separating and purifying flavone from wormwood through imitating moving bed chromatography. It comprises following steps: measuring 0.7- 1.0 kg dried and disintegrated wormwood, adding 10- 15kg 80% ethanol at constant temperature for water bath refluxing and extracting, filtering, combining filterate; extracting for two times, washing residuel, combining with washing liquid, defatting with ligarine, depressing and condensing and getting coarse wormwood ligarine extract; preparing feed liquid with said coarse extract and feeding it to imitating moving bed for separation and purification; dissloving said coarse extract with methanol, filtering with organic filtering membrane of 0.45 mu m and getting feed liquid for imitating moving bed with concentration being 100- 300 mg / ml; the washing liquid is mixture of methanol and water, the volumetric ratio between methanol and water is 40: 60; condensing extract liquid to saturation, reducing temperature gradually to 4- 10 Deg. C below zero, crystallizing for 5- 6 hours, filtering, washing crystal for two times, vacuum drying and getting pure wormwood flavone. The invention is characterized by increased production automation degree, improved working efficiency and production working condition.

Description

technical field [0001] The invention relates to a method for separating and purifying natural medicines, in particular to a method for separating and purifying flavonoids from Artemisia annua by simulated moving bed chromatography. Background technique [0002] Artemisia annua, also known as Artemisia quinoa, Artemisia water, Artemisia perenniale, etc., is a perennial herb of Compositae, distributed all over the country, and is a wild vegetable with flavor. The medicinal value of Artemisia annua has been recorded as early as in ancient my country. It has medicinal effects such as clearing away heat, eliminating rheumatism, and appetizing. The current method for extracting flavonoids in Artemisia annua is soaking, filtering, and extracting to obtain crude products, but further purification is required to meet the medicinal requirements. Currently, the commonly used purification method is column chromatography with neutral alumina as a filler. Column chromatography is a commo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/30C07H17/07A61K36/28
Inventor 张大兵王亚辉孟强王艳
Owner JIANGSU HANBON SCI & TECH CO
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