The invention discloses an aqueous neoprene type environment-friendly glue which comprises aqueous rosin emulsion with low grain diameter and high stability, aqueous acrylic resin emulsion, ethane-vinyl acetate emulsion, anion type neoprene latex, aqueous foam suppressor, wetting dispersant, preservative, tackifier, nanometer calcium carbonate concentrated slurry, aerosol, and the like. The invention also discloses a preparation method of the aqueous neoprene type environment-friendly glue; the aqueous neoprene type environment-friendly glue takes water as a dispersed media, is uniform milky liquid, has the comprehensive advantages of no organic solvent, no heavy metal, insipidity, good initial adhesive force, excellent bond strength, excellent hold tack, excellent heat resistance and aging resistance, short drying time, long opening time, large spray area of unit weight, glue saving, excellent oily leather resistance, fireproofing, safe transportation, and the like, has the advantagesof reducing environment pollution, improving operation and construction environment, saving large amounts of organic fuel, insipidity, and the like, is widely applied to industries of soft furnitureof cases and bags, sofas, swivel chairs, mattresses, pillows, folding screens, and the like, and is especially suitable for application of green high-grade export products of occident.