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53 results about "Cell junction" patented technology
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A cell junction (or intercellular bridge) is a type of structure that exists within the tissue of some multicellular organisms, such as animals. Cell junctions consist of multiprotein complexes that provide contact between neighboring cells or between a cell and the extracellular matrix. They also build up the paracellular barrier of epithelia and control the paracellular transport. Cell junctions are especially abundant in epithelial tissues.
Bone tissue repair ink, composition and bracket, and preparation methods thereof as well as kit
ActiveCN109381749APromote proliferationPromote directed differentiationAdditive manufacturing apparatusTissue regenerationBone tissueBone tissue engineering
The invention relates to the field of bone tissue engineering, in particular to bone tissue repair ink, a bone tissue repair composition and a bone tissue repair bracket, and preparation methods thereof as well as a kit. The bone tissue repair ink and a bioactive carrier wrapping cells are used as raw materials, and the bone tissue repair bracket is printed according to a preset three-dimensionalstructure by adopting a biological printer. Meanwhile, living cell printing is realized by adopting the bone tissue repair ink and the bioactive carrier as the raw materials. The cells are uniformly distributed on a bracket model formed by the bone tissue repair ink and difficultly slide down to the bottom of the bracket, so that the problem that the expression of some characteristic proteins of the cells is easily lost in the prior art is effectively solved. The growth of the cells on the bracket is facilitated. A human body bone cell growth environment is better simulated, the cell proliferation, the directional differentiation and the specific protein expression are promoted, the extension and the migration of the cells in the bone tissue bracket and the cell junction establishment arefacilitated, and an organic construction body is formed.
Owner:REGENOVO BIOTECH
Fusion protein arrays on metal substrates for surface plasmon resonance imaging
InactiveUS20030100127A1Material nanotechnologyPeptide librariesCell adhesionSurface plasmon resonance imaging
Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an omega-modified alkanethiol monolayer to the substrate and then contacting the omega-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.
Owner:WISCONSIN ALUMNI RES FOUND
Antigenic modulation of cells
InactiveUS8007784B1Less anti-ALess-proneBiocideSnake antigen ingredientsPolyethylene glycolDecreased Phagocytosis
The present invention is directed to a non-immunogenic cellular composition comprising: a cell having a cell surface and antigenic determinants on the cell surface; an optional linker molecule covalently attached to the cell surface; and a hydrophilic, biocompatible, non-immunogenicity providing compound or polymer (e.g., polyethylene glycol or a derivative thereof) covalently attached to the linker molecule or directly to the cell. In one embodiment, the linker molecule is covalently attached directly to the antigenic determinant on the cell surface. In an alternate embodiment, the linker molecule may be covalently attached to a non-antigenic site on the cell surface, but will camouflage the antigenic determinant on the cell surface. Various uses of the resulting non-immunogenic cell are also provided, including a method of decreasing phagocytosis of a cell, a method of decreasing an adverse reaction to a transfusion, a method of decreasing rejection of a transplanted cell, tissue or organ, and a method of decreasing antibody-induced aggregation of cells.
Owner:ALBANY MEDICAL COLLEGE
Preparation method of tissue engineering skin containing appendant organs
InactiveCN101773688AIncrease success rateMaintain structure and functionEpidermal cells/skin cellsArtificial cell constructsSweat glandTissue engineered skin
The invention relates to a preparation method of tissue engineering skin containing appendant organs. In the invention, the preparation method comprises the following steps of: inoculating amniotic mesenchyme stem cells, amniotic mesenchyme stem cells induced to the directions of hair papillae, amniotic epithelial cells and amniotic epithelial cells induced to the directions of the epithelia of the sweat gland into a gel solution, then inoculating the amniotic epithelial cells and the amniotic epithelial cells induced by keratinocyte in a warp direction on the surface of a structure of the hair follicle and the sweat gland after culturing by a dermal layer by induced culture forming the structure of the hair follicle and the sweat gland, and obtaining the tissue engineering skin with the structure of the hair follicle and the sweat gland after culturing. The skin has elasticity, toughness, vigorous cellular metabolism, strong multiplication capacity, sufficient secretion of extracellular matrices, tight linkage of the cuticular layer, the inophragma and the cells of the dermal layer and little possibility of falling off, enhances the success rate of transplantation, can promote the healing of a wound surface, enhances the effects of restoration and replacement and has the function of regulating the immunological rejection of a receptor; by the contained structure of the hair follicle and the sweat gland, the function of the skin is more complete; the adopted amniotic tissues are postpartum wastes and have extensive source, strong cell multiplication capability and multiplepassage frequency; and the invention is beneficial to the mass production of seed cells and lowers the industrialization cost.
Owner:FOURTH MILITARY MEDICAL UNIVERSITY +1
MRAM field-inducing layer configuration
InactiveUS6891193B1Accurate and uniform write selectivityImprove equipment reliabilitySolid-state devicesDigital storageStatic random-access memoryHigh density
A magnetic random access memory (MRAM) device is provided which includes a conductive line configured to induce a magnetic field with a higher magnitude along at least a portion of a magnetic cell junction than along a spacing arranged adjacent to the magnetic cell junction. In some embodiments, the conductive line may include first portions aligned with a plurality of magnetic cell junctions and second portions aligned with spacings arranged between the plurality of magnetic cell junctions. In such an embodiment, the first portions preferably include different peripheral profiles than the second portions. A method for fabricating such an MRAM device is also provided herein. The method may include aligning magnetic cell junctions and first portions of a field-inducing line with each other such that at least part of the first portions of the field-inducing line are configured to conduct a higher density of current than second portions of the field-inducing line.
Owner:CYPRESS SEMICON CORP
Structure and method of bus-structure-based embryonic biomimetic self-healing
ActiveCN102662805AReduce consumptionEasy wiringRedundant hardware error correctionSelf-healingEmbedded system
The invention discloses a structure and a method of bus-structure-based embryonic biomimetic self-healing. The self-healing structure is provided with a bus array. The bus array comprises working cells, backup cells, an input cell, an output cell and a bus. All the cells are connected to the bus in parallel. The input cell is connected to the bus at the input end of the bus array. The output cell is connected to the bus at the output end of the bus array. All the cells of the self-healing structure are connected to the bus, so that wiring is simple. A configuration memory stores no configuration information redundancy but configuration information of the cells, so that consumption of storage resources is low. A self-healing mechanism requires direct intervention of failing cells and the backup cells only, configuration information and routing of other cells are unchanged, and the efficiency is higher.
Owner:NAT UNIV OF DEFENSE TECH
Laminous Vascular Constructs Combining Cell Sheet Engineering And Electrospinning Technologies
ActiveUS20150112419A1Prevent acute thrombosisLong-term patencyStentsElectro-spinningCell sheetBlood vessel
Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.
Owner:WAKE FOREST UNIV HEALTH SCI INC
Device with recessed tracks for forming a cellular network
InactiveUS20050002985A1Reduce decreaseGood continuityNervous disorderMicrobiological testing/measurementCell-Extracellular MatrixLaminin
A device for forming a cellular network is provided. The device comprises a substrate. A plurality of hydrophilic sites are provided on the substrate for occupation by cells. A plurality of hydrophilic tracks are provided on the substrate for guiding formation of cell connections between sites. Also provided are a plurality of hydrophilic regions. The hydrophilic sites and hydrophilic tracks are recessed between the hydrophobic regions. The hydrophilic sites and / or hydrophilic tracks may be provided with a guidance cue for guiding formation of the cellular network. Preferably the guidance cue is a chemical guidance cue, such as an extracellular matrix (ECM) protein. An example of an ECM protein is laminin. The device may be used for forming a cellular network of neurons.
Owner:QINETIQ LTD
Method of Fabricating Semiconductor Device
ActiveUS20080268624A1Stress minimizationSolid-state devicesSemiconductor/solid-state device manufacturingDielectricIon implantation
This invention relates to a method of fabricating a semiconductor device. A P well for a cell junction may be formed by performing an ion implantation process employing a zero tilt condition. Stress caused by collision between a dopant and a Si lattice within a semiconductor substrate may be minimized and, therefore stress remaining within the semiconductor substrate may be minimized. Accordingly, Number Of Program (NOP) fail by disturbance caused by stress remaining within a channel junction may be reduced. Further, a broad doping profile may be formed at the interface of trenches by using BF2 as the dopant when the P well is formed. A fluorine getter layer may be formed on an oxide film of the trench sidewalls and may be used as a boron diffusion barrier. Although a Spin On Dielectric (SOD) insulating layer may be used as an isolation layer, loss of boron (B) may be prevented. Accordingly, an additional ion implantation process for compensating for lost boron (B) may be omitted and a NOP disturbance characteristic may be improved.
Owner:SK HYNIX INC
Fusion protein arrays on metal substrates for surface plasmon resonance imaging
InactiveUS7067322B2Fast wayMaterial nanotechnologyPeptide librariesCell adhesionSurface plasmon resonance imaging
Disclosed are methods for making surface plasmon resonance-capable arrays wherein molecules, such as proteins or nucleic acids, or cells, are adhered to a metal substrate. The metal substrates are modified by depositing an ω-modified alkanethiol monolayer to the substrate and then contacting the ω-modified monolayer with a heterobifunctional linking compound. Biomolecules or cells can then be attached to the heterobifunctional linking compound. Also disclosed are arrays wherein glutathione-containing molecules are immobilized on the substrate and GST-containing molecules are then specifically immobilized onto the substrate, taking advantage of the affinity between glutathione and GST.
Owner:WISCONSIN ALUMNI RES FOUND
Porous chitosan microcarrier as well as preparation method and application thereof
ActiveCN108047482AKeep aliveGuaranteed functionHepatocytesArtificial cell constructsPorosityCulture cell
The invention discloses a porous chitosan microcarrier as well as a preparation method and an application thereof. The porous chitosan microcarrier takes chitosan as a main component, the diameter ofthe porous chitosan microcarrier is 100-300 mu m, interconnected pores are formed in the surface and the interior of the porous chitosan microcarrier, the diameter of the pores is 10-70 mu m, and theporosity is 95% or higher. The porous chitosan microcarrier is uniform in size, the interconnected pores are formed in the surface and the interior, and the highest porosity can reach 95% or higher. With adoption of the porous chitosan microcarrier for culturing cells, the cells can not only be adhered to the surface of the carrier, but also can enter the internal pores to grow, effective cell-cell connection is formed in three-dimensional space, real three-dimensional culture is realized, in vivo environment is effectively simulated, vitality and function of cells are better maintained, and the microcarrier has good biocompatibility.
Owner:EZHOU INST OF IND TECH HUAZHONG UNIV OF SCI & TECH +1
Cryopreservation method for protecting cell junction
InactiveCN102487938AImprove toleranceFree from damageDead animal preservationCanine kidneyCultured cell
The invention discloses a cryopreservation method for protecting cell junction. The method comprises the following steps of: a) culturing Madin Darby canine kidney (MDCK) cells; b) dividing the MDCK cells in the step a) into a normal control group, a cryopreservation group, a trehalose group, an Hsp70 high expression group and an Hsp70+ trehalose group; c) performing filter culture on the normal control group, the cryopreservation group and the trehalose group respectively, performing filter culture and gene transfection on the Hsp70 high expression group, and performing filter culture and gene transfection on the Hsp70+ trehalose group; d) performing cryopreservation on the normal control group, the cryopreservation group, the trehalose group, the Hsp70 high expression group and the Hsp70+ trehalose group; e) growing cells and performing morphologic observation (the cultured cells are observed by a microscope and are subjected to HE staining); f) testing the survival rate of the cells; g) observing and analyzing the cell junction morphology and structure; and h) performing a fluorescein experiment. The method is simple, and convenient to use; and the structure and function of the cell junction after tissues and organs are subjected to cryopreservation can be kept complete, and the damage of the cell junction can be effectively avoided.
Owner:HOSPITAL ATTACHED TO QINGDAO UNIV
Laminin-derived multi-domain peptides
Multi-domain peptides including a heparin-binding peptide sequence covalently linked to a linker sequence, which linker sequence is covalently linked to a trifunctional amino acid residue forming a branch with two arms, with substantially similar (homodimeric) cellular attachment peptide sequences covalently linked, directly or through an intermediate, to each branch arm, where the sequences are cell binding analogs of or derived from laminin or a portion of laminin. Further provided are preparations for medical devices, pharmaceutical compositions and methods of using the same.
Owner:FERRING INT CENT SA
Device and method for determining expansion frequency points of cell
InactiveCN101860868AImprove expansion efficiencySimple calculationNetwork planningEngineeringCell junction
The invention relates to the field of mobile telecommunication, in particular to the technology for expanding frequency of a base station of a cell. The invention provides a device and a method for determining expansion frequency points of the cell. The method comprises the following steps of: determining a neighboring cell of the cell to be expanded; determining signal intensity of all the frequency points of the neighboring cell on the junction of the neighboring cell and the cell to be expanded; and determining the expansion frequency points of the cell to be expanded according to the determined signal intensity. Compared with the signal intensity of the central point of the cell, the signal intensity of the neighboring cell on the cell junction more completely reflects the strength of the interference of all the frequency points of the neighboring cell with the cell to be expanded, so that the expansion frequency points of the cell to be expanded can be selected according to the signal intensity to make the interference strength as small as possible. The method for determining the expansion frequency points does not depend on changing-over relation definition and changing-over statistics of the conventional networks to select the expansion frequency points, has simple calculation process, can quickly select expansion frequency and improves frequency expansion efficiency.
Owner:CHINA MOBILE GROUP SHANDONG
Method for measuring silicon solar cell junction depth
InactiveCN101281935AJunction depth can be measuredSemiconductor/solid-state device testing/measurementFinal product manufactureHydrofluoric acidSilicon solar cell
The invention relates to a measuring method for a silicon solar cell junction depth, which belongs to field of silicon solar cell performance parameter measurement method. The measuring method measures and calculates the silicon solar cell junction depth by employing anode oxidation method and differential weighing calculation method, and includes steps: measuring weight of uneroded phosphorus diffused layer silicon substrate namely cell sheet and square resistance, oxidizing surface of the silicon substrate by the anode oxidation method to obtain a oxidation layer; etching the generated oxidation layer by hydrofluoric acid and measuring the square resistance, repeating the upper step until the square resistance becomes smaller, weighting the silicon substrate. Difference value of the weight and the weight measured at beginning is weight of the diffused layer, average depth of the diffused layer namely the cell junction depth can be obtained through dividing the weight difference value by density of the diffused layer and area of the eroded diffused layer.
Owner:SHANXI JINGDU SOLAR ENERGY POWER
Induced pacemaker and Purkinje cells from adult stem cells
ActiveUS9913865B2Quality improvementEasy to transplantGenetically modified cellsNervous system cellsReprogrammingBiological activation
Adult stem cells are reprogrammed to form pacemaker cells and Purkinje cells through the sequential activation of SHOX2>TBX5>HCN2. These Purkinje cells spontaneously surround and connect with the larger pacemaker cells, thus forming an induced sinoatrial body that produces funny current and can make cardiovascular tissues beat in a manner similar to a natural sinoatrial node.
Owner:ALLIANCE OF CARDIOVASCULAR RESERS +1
Application of 4', 5, 7-genistein in preparing rotavirus resisting medicine
InactiveCN103462977APoor resolutionSolve the problem of large toxic side effectsOrganic active ingredientsDigestive systemRotavirus RNABiological activation
The invention discloses application of 4', 5, 7-genistein in preparing rotavirus resisting medicine. The genistein can restrain damage of tyrosine protein kinase to cell junction by restraining activation, caused by rotavirus infection, of tyrosine protein kinase of intestinal epithelial cells, so that the intestinal epithelial cells are prevented from being damaged by rotavirus, the completeness of intestinal mucosa is protected, the symptom of rotavirus diarrhea is obviously reduced, and the lethality is reduced. Thus, when the genistein is used as a main active ingredient, high-efficient and low-toxicity specificity rotavirus diarrhea resisting medicine can be researched and developed hopefully.
Owner:ARMY MEDICAL UNIV
Method and device for locking cell by fixed wireless terminal
InactiveCN104023372AAvoid failureAvoid using effectsAssess restrictionLocation information based serviceTelecommunicationsNetwork service
The invention discloses a method and device for locking a cell by a fixed wireless terminal. The method includes: obtaining current location information of the fixed wireless terminal when a network service enabling instruction arrives; and judging whether distance between the current location information and reference location information is smaller than or equal to a preset distance threshold, if yes, allowing the fixed wireless terminal to initiate the network service normally, and if no, forbidding the fixed wireless terminal to initiate the network service. The method for locking the cell by the fixed wireless terminal adopts a region of a fixed area, as long as customized preset distance thresholds are the same, even if for different fixed wireless terminal, the ranges of locking cells are certainly the same, and the network effective coverage area determined by the method for locking the cell is only related to the reference location and the distance threshold, thereby preventing the problem that cell locking fails or an optimal cell cannot be used at a cell junction, and ensuring accuracy of the range of cell locking.
Owner:ZTE CORP
Induced Pacemaker and Purkinje Cells from Adult Stem Cells
ActiveUS20170182099A1Quality improvementEasy to transplantGenetically modified cellsNervous system cellsReprogrammingBiological activation
Adult stem cells are reprogrammed to form pacemaker cells and Purkinje cells through the sequential activation of SHOX2>TBX5>HCN2. These Purkinje cells spontaneously surround and connect with the larger pacemaker cells, thus forming an induced sinoatrial body that produces funny current and can make cardiovascular tissues beat in a manner similar to a natural sinoatrial node.
Owner:ALLIANCE OF CARDIOVASCULAR RESERS +1
Magnetic memory cell junction and method for forming a magnetic memory cell junction
ActiveUS7199055B2Improve reliabilityTighter switching field distributionNanoinformaticsMagnetic-field-controlled resistorsDopantMagnetic memory
A method for patterning a magnetic memory cell junction is provided herein, which includes etching exposed portions of a stack of layers to a level spaced above a tunneling barrier layer of the stack of layers. In addition, the method may include implanting dopants into exposed portions of the stack of layers. For example, the method may include oxidizing and / or nitriding the exposed portions of the stack of layers. In some embodiments, the steps of etching and implanting dopants may form an upper portion of the magnetic cell junction. Alternatively, the method may include alternating the steps of etching and implanting dopants throughout the thickness of the exposed portions of the stack of layers. In either case, the stack of layers may include a magnetic layer which includes a material adapted to prevent the introduction of dopants underlying the tunneling barrier layer during the step of implanting.
Owner:MONTEREY RES LLC
Functional food for skin aging resistance and wrinkle resistance and preparation method thereof
InactiveCN111053236AHas health effectsWrinkledFood ingredient functionsProtein food ingredientsBiotechnologyWrinkle
The invention discloses a functional food for skin aging resistance and wrinkle resistance and a preparation method thereof. The functional food for skin aging resistance and wrinkle resistance is characterized by comprising the following components in parts by weight: 0.1-10 parts of haematococcus pluvialis, 3-10 parts of vitamin, 16-60 parts of protein peptide and 1-20 parts of a plant powder. The preparation method provided by the invention is simple; the functional food is prepared from haematococcus pluvialis, vitamins, protein peptides and the plant powder. The raw materials are green and natural; no side effect is caused; wherein the components such as haematococcus pluvialis, vitamins, protein peptides and the like have a synergistic effect and have the functions of resisting oxidation and scavenging free radicals, cell connection can be tightened, metabolism speed can be increased, exfoliation of dead skin can be promoted, skin fine wrinkles can be reduced, melanin generationcan be inhibited, and deposition can be prevented;the plant powder possesses health care effect, has a health-care effect on intestines and stomach, can promote digestion of intestines and stomach, expel toxins and beautify faces, and has a positive effect on eliminating wrinkles and fine wrinkles of human bodies.
Owner:广州市中食科康保健食品有限公司
Complexes and methods
InactiveUS20080026413A1Easy to controlExclude influenceArtificial cell constructsVertebrate cellsHeterologousCD20
The invention relates to a cell comprising an exogenous capture moiety on its cell surface, wherein said capture moiety is capable of supporting the attachment of an HLA molecule thereto. In another aspect the invention relates to a cell comprising a capture moiety on its cell surface, and an HLA molecule, wherein said HLA molecule is attached to said cell by means of said capture moiety. Preferably the capture moiety is exogenous, preferably heterologous, prefereably it is CD20. The invention also relates to assays using said cells, and to methods for attaching HLA to them.
Owner:ALEXIS BIOTECH
Method for in-situ observation of cell connection between monolayer cells by using transmission electron microscope
ActiveCN110082380AAvoid damageStable pre-embeddedMaterial analysis using wave/particle radiationConventional transmission electron microscopeCell growth
The invention relates to the technical field of cell observation, and particularly relates to a method for in-situ observation of cell connection between monolayer cells by using a transmission electron microscope. The method comprises the following steps: (1) cell preparation: putting sterilized epoxy resin sheets into a culture plate, centrifuging and resuspending the single-layer cells, uniformly adding the single-layer cells into a substrate with the epoxy resin sheets, and putting the substrate into an incubator for culture; (2) specimen fixing, dehydrating and soaking; (3) area marking,block repairing and pre-embedding; (4) pre-embed block repairing and re-embedding; and (5) slicing, dyeing and electron microscope observation. Through the steps, the original appearance of the monolayer cells can be restored to the greatest extent in situ. Compared with the traditional method, the method has the advantages that the damage to cell connection is reduced; compared with a foreign in-situ embedding method, the resin serving as a carrier for cell growth is simpler and easier to implement compared with a glass slide, and the pre-embedding and re-embedding method is more stable and effective.
Owner:曾琪琪
Method for fabricating semiconductor device with improved refresh time
ActiveUS7265039B2Reduce refresh timeIncrease contact resistanceTransistorSolid-state devicesDopantSemiconductor
The present invention relates to a method for fabricating a semiconductor device with improved refresh time. The method includes the steps of: forming a plurality of gate lines on a substrate; forming a plurality of cell junctions by ion-implanting a first dopant with use of the gate lines as a mask; forming a buffer layer along a gate line profile; and forming a plurality of plug ion-implantation regions in the cell junctions by ion-implanting a second dopant into the substrate under the presence of the buffer layer to thereby from the plugs thereon.
Owner:SK HYNIX INC
Connector assembly of solar photovoltaic cell junction box
InactiveCN101854043AReduce resistanceIncrease profitCoupling device detailsPhotovoltaicsCopperHigh transmission
The invention discloses a connecting assembly of a solar photovoltaic cell junction box, which is characterized in that the connector assembly is in an integrated structure and comprises a diode inserting frame, a cable wiring port and a refluxing strip connecting port, wherein the cable wiring port and the refluxing strip connecting port are integrated with the diode inserting frame; the diode inserting frame is positioned between the cable wiring port and the refluxing strip connecting port; a diode inserting port is arranged in the diode inserting frame; and the diode inserting port is a hollow cylinder formed by oppositely folding and turning a copper piece (sheet) with two semicircular concave surfaces. In the invention, because the connector assembly is changed from combination into integration of a plurality of parts and the connection between the inserting port and each diode pin is changed from line contact or point contact into surface contact, and the connector assembly has the advantages of small current resistance, high transmission efficiency, simple machining and installing processes, low consumption of copper product resources and low production cost.
Owner:刘绍东
Preparation method of tissue engineering skin containing appendant organs
InactiveCN101773688BIncrease success rateMaintain structure and functionEpidermal cells/skin cellsArtificial cell constructsEpitheliumCell-Extracellular Matrix
Owner:FOURTH MILITARY MEDICAL UNIVERSITY +1
Cell sheet protection liquid
The invention relates to the field of tissue engineering and regenerative medicine. Specifically, the invention provides a cell sheet protection liquid capable of maintaining the activity and cytokinesecretion ability of cells in a cell sheet during storage and transportation of the cell sheet, maintaining the integrity of the cell sheet and cell junction in the sheet, and protecting the cells from apoptosis. The cell sheet protection liquid comprises the following components: a, a buffer salt solution, b, an extracellular matrix component, c, a reducing agent, d, a substance required by cellmetabolism, and e, a hormonic substance. The invention also relates to the use of the cell sheet protection liquid in the storage and transportation of cell sheets.
Owner:BOE TECH GRP CO LTD +1
Laminous vascular constructs combining cell sheet engineering and electrospinning technologies
ActiveUS10092679B2Prevent acute thrombosisLong-term patencyStentsElectro-spinningVascular structureCell sheet
Vascular scaffolds and methods of fabricating the same are disclosed for tissue engineering of vascular constructs. By combining electrospun matrices with cell sheet technologies, vascular constructs with more mature cell layers can be obtained for reconstruction of blood vessels, heart valves and the like. A engineered smooth muscle cell sheet, wrapped around an electrospun vascular scaffold, is able to provide a mature SMC layer that expresses strong cell-to-cell junction markers and contractile proteins. In addition, preconditioning of the cell sheet covered vascular scaffold maintained cell viability and infiltration into the scaffold.
Owner:WAKE FOREST UNIV HEALTH SCI INC
High-throughput single cell capture and arrangement chip and method
ActiveCN108165475AIncrease catch rateSimple methodBioreactor/fermenter combinationsCell dissociation methodsCell–cell interactionCell engineering
The invention relates to a high-throughput single cell capture and arrangement chip and method and belongs to the field of materials and tissue engineering and particularly relates to high-throughputcapture and arrangement of single cells. The method comprises the following steps: making a substrate surface micro-pattern under the action of protein penetration, performing the high-throughput capture and arrangement of the single cells under the action of membrane pore limitation, and arranging and paving the cells on the complex micro-pattern. The high-throughput single cell capture and arrangement chip and method have the benefits that the capture rate of 85 percent or above of single cells can be realized, and the orderly arrangement and the orderly pavement of the cells on the specified protein pattern are realized; the influence caused by cellular heterogeneity during the large cell population study is avoided, and the method is simple and high in single cell capture rate, has a suitable follow-up culture environment, can be determined to be applied to the study on cell junction, intercellular interaction, cell signal transduction and the like at a single cell level and has avery important effect in the cell engineering and tissue engineering study.
Owner:BEIHANG UNIV
Photovoltaic cell junction box
The invention provides a photovoltaic cell junction box comprising a labelling device and a label storage cavity formed in the labelling device. A label storage mechanism is arranged in the label storage cavity. The label storage mechanism can paste stored labels to junction boxes. The label storage cavity further internally comprises a pasting mechanism. The pasting mechanism can paste the labelsto the surfaces of the junction boxes and conduct pressing. A junction box transport cavity is formed below the label storage cavity. A junction box transport mechanism is arranged in the junction box transport cavity. The device is simple in mechanism and easy and convenient to use, and the labels can be automatically pasted to the surfaces of the junction boxes. According to the device, the junction boxes are evenly conveyed out by driving conveyor belts through small-angle rotation of a swinging sector gear; during work, under the condition that no junction box arrives within a long periodof time, none of main working parts of the device operate, and the electric power resource can be saved in a limited manner; and power input of the device is achieved through only one motor, and thedevice cost is reduced.
Owner:故城县中太新能源科技有限公司
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