The invention discloses a meat flavor composition which comprises 4-methyl-5-(2-hydroxyethyl)-thiazole, 4-methyl-5-(2-hydroxyethyl)-thiazole-acetate, 2-methyl-3-furanthiol-acetate, 2-methyl-butyraldehyde, 2-methyl-furanthiol, 1,6-ethanthiol, 2-acetyl-4-methyl-thiazole, 2,4-decadienal, methyl mercatopropanol, 2,6-dimethoxyphenol, clove oil, butyl lactate, 3-methyl mercatopropanol, 2-methyl-2-mercapto-undecane, 3-tert-butyl-4-hydroxyanisole, 2,3,5-trimethylpyrazine, 2-methyl-3-mercaptofuran, ethyl maltol and the like, with the balance being an oil-soluble solvent. The invention also provides a preparation method for the composition.