66results about How to "Scale up production" patented technology

The invention discloses a synthetic method for semaglutide and belongs to the field of polypeptide synthesis. In the method, amino acids at 1-30 positions of a main sequence of the semaglutide is obtained by adopting solid phase synthesis, Val at 10 position and Ser at 11 position adopt Fmoc-Val10-Ser11 (Psi(Me, Me) pro)-OH; and an Lys side chain at 20 position adopts Fmoc-Lys(X)-OH. The method comprises the following steps of performing cracking precipitation to obtain a semaglutide crude peptide; and performing purification to obtain the semaglutide. In the method, by introducing the Fmoc-Val10-Ser11 (Psi(Me, Me) pro)-OH to solve the problem of difficult sequence synthesis of the semaglutide, difficult sequence synthesis becomes simple and easy; and meanwhile, the yield is greatly increased, the purity of the crude peptide is greatly improved, the production cost is greatly lowered, and industrial amplification production is facilitated.

The invention relates to a preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in a targeted manner. The preparation method comprises the following steps: (1) by taking water as a solvent, preparing a PVA / PEI (Poly(Vinyl Alcohol) / Polyethylenimine) spinning solution and carrying out electrostatic spinning; drying and carrying out vacuum cross-linking treatment in a dyer containing a glutaraldehyde solution to obtain PVA / PEI nano fibers with good water stability; (2) adding activated hyaluronic acid into the PVA / PEI nano fibers with the good water stability; reacting on a shaking bed to obtain electrostatic spinning PVA / PEI nano fibers which are functionally modified by the hyaluronic acid; then adding triethylamine and acetic anhydride to carry out acetylation treatment on all residual amino groups on the surface of a PVA / PEI nano fiber film; and finally, washing and drying to obtain the product. The preparation method provided by the invention is low in raw material price and simple in preparation process; the prepared hyaluronic acid functionalized electrostatic spinning nano fibers have good appearance, structural stability, biocompatibility and blood compatibility and the application prospect is wide.

The invention discloses a preparation method of coal-based porous carbon based on potassium carbonate cyclic activation and a preparation method of biomass-based porous carbon, relates to a preparation method of the coal-based porous carbon and a preparation method of the biomass-based porous carbon, and aims to solve the problems that the pore structure of a porous activated carbon material obtained by a physical activation method is not developed, and the amount of an activating agent required by a chemical activation method is large. The preparation method comprises the following steps: sequentially crushing, grinding and screening raw materials, then carrying out ball milling mixing or liquid phase impregnation to mix a small amount of K2CO3 with a coal matrix, carrying out high-temperature activation on the obtained mixture in an atmosphere furnace, and cleaning and drying the activated product. According to the preparation method, the potassium carbonate can catalyze the activation reaction of an activation gas and the carbon matrix, so that the pore forming process is enhanced; in addition, the cyclic regeneration of the potassium-based component can be realized in the reaction process, so that coal or biomass-based porous carbon with developed pores can also be obtained under the addition of a small K2CO3 adding amount. The method is suitable for preparing the porous carbon.

This invention relates to a spray-pyrolysis process for preparing red fluorescent powder, whose chemical formula is (Li1-aNaa) (Eu1-b-c-dSmbYcGdd)(SiO2)1 / 6(W1-mMom)2O8, wherein, a is 0-0.8; b is 0-0.08; c is 0-0.08; d is 0-0.08; m is 0-1. The spray-pyrolysis process comprises: weighing soluble compounds of the cations according to the above chemical formula, preparing clear precursor solution, atomizing, reacting, and collecting the red fluorescent powder. The red fluorescent powder is approximately spherical, and has such advantages as high crystallinity, uniform particle size distribution, smooth particle surface, high luminescent efficiency, and high color purity. The spray-pyrolysis process has such advantages as simple process, easy operation, and high production efficiency, and is suitable for continuous production.

The invention relates to a preparation method of grafted starch. The preparation method comprises the following steps of firstly adopting an electrical Fenton method to pretreat a prepared starch milk suspension for breaking a starch crystalline region, so that molecular chains of starch molecules are stretched, and more reactive sites capable of being in graft polymerization are exposed; then dropwise adding grafting monomers in a pretreated system, continuously introducing oxygen and electrifiying, enabling generated hydroxyl radicals as an initiator to initiate a graft polymerization reaction of starch molecule free radials and the grafting monomers, and meanwhile avoiding the generation of homopolymers of the grating monomers as much as possible. Grafting treatment is carried out on starch by adopting the method, the grafting ratio can reach 69.2%, the grafting efficiency can reach 87.6%, and the content of the homopolymers is low; the grafted starch prepared by the method has a high water-absorbing quality or film-forming property; and besides, the method is simple in technological operation and post-treatment, mild in reaction condition, time-saving, efficient and little in environmental pollution, and is beneficial for expanding scale production.

The invention discloses a nitrogen-doped porous cobalt-based nano material and a preparation method and application thereof. The material is prepared by taking a ZIF-67@ZIF-8 composite material as a precursor through high-temperature calcination. The material is used as a catalyst, water is used as a solvent, atmospheric pressure oxygen is used as an oxidizing agent, ammonia water is used as a nitrogen source, and conversion from primary alcohol to nitrile compounds can be realized in a one-step method at the temperature of 50 DEG C. According to the invention, the nitrogen-doped porous cobalt-based nano material with large specific surface area and pore volume and good alkalinity and hydrophilicity is adopted as a catalyst, and the catalyst is simple to prepare and is efficient; reactionconditions are mild, and high-temperature and high-pressure conditions are not needed; alkali and strong oxidant do not need to be sued in reaction; water as a reaction solvent is also the unique by-product of the reaction; the substrate has a wide application range and can be applied to aromatic ring, fused ring and heterocyclic primary alcohol compounds; and the reaction can be carried out at high concentration, and the catalyst can be recycled after the reaction is finished, so that the industrial application is facilitated.

The invention provides a production technology for extracting soybean isoflavone aglycone through an enzymatic hydrolysis method, and belongs to the technical field of processing and extraction of soybeans. The technology comprises the following steps: immersing degreased soybean meal according to a material-to-water ratio of 5-10:1; adding ethanol to an immersion solution until the mass concentration of ethanol in the ethanol added immersion solution is 10%; hydrolyzing through adding superhigh temperature beta-glucosidase to the immersion solution at 90-120DEG C for 1-2h under slowly stirring; carrying out solid-liquid separation after the hydrolysis; extracting through adding 60-80% ethanol to the resulting solid precipitate; filtering the resulting extract solution by a film to obtain a permeation solution; and carrying out protein removal, reduced pressure concentration and drying on the permeation solution to obtain the isoflavone aglycone. The technology which utilizes enzymological characteristics comprising the high temperature resistance, the heat stability and the like of the superhigh temperature beta-glucosidase enables the superhigh temperature beta-glucosidase to be applied to the extraction and the preparation of the soybean isoflavone aglycone, so the production efficiency is improved, the production cost is reduced, and the obtained isoflavone aglycone is sugar-free isoflavone having a high absorptivity and a strong biological activity.

The invention belongs to the technical field of fine chemical engineering, and particularly relates to a green preparation method for metal oxide nano material. A 'metal ion-thiocyanide' system is used as a precursor, and reacts with aqueous alkali at the room temperature to form a product. The prepared metal oxide nano material is packed in a sealed manner to form the product. Various metal oxidenano materials, various metal oxide composite nano materials and doped nano materials can be synthesized. The preparation method has universality, the process is simple, conditions are gentle, cleanproduction and transportation can be realized, and production scale can reach industrial scale. The prepared metal oxide nano material can serve as an advanced functional material and a chemical novelmaterial, and application value is obtained in extensive fields.

The invention provides a multiple-phase thermally driven heat transfer element. The heat transfer is realized by adopting the working medium single phase inside a sealed cavity body. At least one part of the cavity body is a heat absorbing end (part); at least the other part of the cavity body is a heat discharging end (part). In the heat transfer process, the working medium inside the cavity body keeps at least two-phase or multiple-phase composite heat transfer flow body; in the two-phase operation, the heat transfer is realized by keeping a way of other two phases such as solid and liquid, which is different from the vapor-liquid phase-change circulation of the prior heat pipe; meanwhile, the heat transfer can be also realized by three phases or more phases to realize the transfer of the heat energy; in the process of transferring the heat energy, only the heat energy provided by the heat source can realize the heat energy transfer without consuming the external energy.

The invention provides a strategy for obtaining a novel high selenate tolerant strain by utilizing selenate to treat tea endophytic herbaspirillum spp WT00F and combining with a forced evolution method, and a method of the novel high selenate tolerant strain to produce red elemental selenium (Se<0>). Through selenate treatment and forced evolution, the tea endophytic herbaspirillum spp can be repeatedly transferred and cultured in a 200 mM selenate culture medium, so that the stable novel high selenate tolerant strain- tea Herbaspirillum sp.NTC00F can be finally formed, and selenate tolerancecan reach 800 mM. The tea Herbaspirillum sp.NTC00F is placed into a culture medium containing 400 mM of selenate to ferment; and the bacteria can reduce the selenate and efficiently produce the red nano elemental selenium, and the yield of the red nano elemental selenium can reach 1,000 [mu]g / ml. The prepared pure red nano elemental selenium can be used for pharmaceutical, agricultural and food processing, and is simple in technical operation, low in cost, green, environmentally-friendly and pollution-free.

The invention discloses a synthesizing method of a carbolin compound. The synthesizing method comprises the following steps of enabling pyridine derivatives, o-nitroaniline and o-nitroaniline derivative thereof to generate coupling and reduction reaction, or directly generate coupling reaction with benzotriazole; converting into a diazotized triazole intermediate; finally, treating the obtained intermediate after reaction under the action of a reagent, so as to obtain substituted carbolin. The synthesizing method has the characteristics that on the basis of the improved Graebe-Ullmann method,the reaction raw materials, reaction reagents and conditions are preferably selected; the selectivity of target products is enhanced, the reaction time is shortened, the reaction temperature is reduced, the number of impurities is fewer, the yield rate is high, and the like; the method is suitable for synthesizing the substituted alpha-carbolin and beta-carbolin, so as to provide a simple and reliable selection plan for the obtaining and utilization of the compound.

The invention provides a bacillus fermentation medium for efficiently producing short branched fatty acid by waste cakes and bean dreg resources and a fermentation process. Efficient production of theshort branched fatty acid is achieved by matching specific medium compositions with culture conditions. A fermentation medium formula comprises, 10-100g / L of corn starch, 20-100g / L of waste organic nitrogen sources, 1-10g / L of ammonia chloride, 3-15g / L of calcium carbonate, 1-10g / L of dipotassium phosphate, 1-10g / L of magnesium sulfate, 0.005-0.10g / L of manganese sulfate and pH (potential of hydrogen) is 6-8. Fermentation culture conditions include that culture temperature is 25-40 DEG C, the rotating speed of a shaking table is 180-240r / min, and fermentation time is 30-60 hours. According tothe fermentation medium, low-cost soybean residue, rape seed cakes and cottonseed cakes serve as fermentation raw materials. Under the process conditions, the yield of the short branched fatty acid is increased by fermentation verification of geobacillus, bacillus subtilis and bacillus amyloliquefaciens. The process has the advantages that the process is simple in assembly, environmentally friendly, economical, high in yield, stable and wide in applicable range. The fermentation medium has a certain application prospect.

The invention discloses a method for preparing 3-trifluoromethyl pyrrole boric acid, belonging to the technical field of organic chemical synthesis. The method is characterized by comprising the following steps: (I) with trifluoroacetic anhydride as a start raw material, generating 4-ethoxy-3-alkenyl trifluoroacetyl ketone through reaction between the trifluoroacetic anhydride and ethyl vinyl ether; (II) performing heating reflux of 4-ethoxy-3-alkenyl trifluoroacetyl ketone and hydrazine dihydrochloride in an ethanol solution to obtain 3-trifluoromethyl pyrrole; and (III) performing functional group conversion of 3-trifluoromethyl pyrrole to generate 1-methyl-3-trifluoromethyl-5-pyrrole boric acid, N-tert-butyl acyl-3-trifluoromethyl-5-pyrrole boric acid and 1-methyl-3-trifluoromethyl-4-pyrrole boric acid respectively. The method has the beneficial effects that the generated target compound has good purity and stable properties, the method is simple and easy to operate, and the preparation scale is easy to enlarge to realize industrial production.

The invention discloses a method for preparing high-purity sucrose fructan monomers by high-speed counter-current chromatography, which comprises the following steps: protecting hydroxyl of fructooligosaccharide to obtain fructooligosaccharide derivatives, carrying out high-speed counter-current chromatography to separate the fructooligosaccharide derivatives to obtain a kestose derivative, a nystose derivative and a glucopyranoside derivative, removing the hydroxyl protecting group to obtain the high-purity kestose, nystose and glucopyranoside. The purities of the kestose, nystose and glucopyranoside are respectively higher than 98%. The method has the advantages of short separation time, high preparation quantity,, high recovery rate, no sample loss, mild separation environment and solvent saving, and can implement scale-up production.

The invention relates to a preparation method for a CT (computed tomography) contrast media based on the second-generation polyamide-amine dendrimer / gold nanoparticles. The preparation method comprises the following steps of: weighing the second-generation polyamide-amine dendrimers with tail ends which are amino groups in aqueous solution, adding chloroauric acid solution in a water-bath condition, and magnetically stirring and reacting for 3 hours; cooling at a room temperature and then performing acetylation treatment; dialyzing the solution after the reaction, performing freeze-drying treatment to obtain a final product; and evaluating the in-vivo and in-vitro CT contrast performances of the product. The product prepared by the preparation method disclosed by the invention is simple and environment-friendly in preparation process, good in stability and cytocompatibility, and capable of showing a good in-vivo CT contrast effect.

The invention belongs to the technical field of novel chemical materials and in particular relates to an insoluble inorganic salt micro-nano material as well as a preparation method and application thereof. The insoluble inorganic salt micro-nano material is synthesized by taking a metal ion- thiocyanide system as a precursor and taking the precursor to react with a water-soluble inorganic salt solution at room temperature, wherein when water-soluble inorganic salt is sodium carbonate and metal ions are calcium ions, the prepared inorganic salt micro-nano material is a calcium carbonate micro-nano material with a special shape; when the metal ions are indium ions, the prepared insoluble inorganic salt micro-nano material is an indium salt nano material. The calcium carbonate micro-nano material with the special shape can be used as a chemical material and a filling agent and is used for fields including rubber, plastic, coating, cosmetics and the like; the insoluble indium salt nano material can be used as a functional addition material and a doping material and is used for fields including functional materials, photoelectric functional devices and the like.

The invention discloses a magnetic responsive photonic crystal, and a simple large-scale preparation method and an application thereof. The structure of the magnetic responsive photonic crystal comprises a one-dimensional stable nano-chain structure assembled by Fe3O4 colloidal nanoparticles under the action of an external magnetic field; the Fe3O4 colloidal nanoparticles comprise aggregated Fe3O4nanocrystals, the surfaces of the Fe3O4 colloidal nanoparticles are covered with poly(4-styrenesulfonic acid-co-maleic acid) sodium salt (the molar ratio of benzenesulfonic acid to maleic acid is 3:1), and the highest Zeta potential value of the surfaces of the Fe3O4 colloidal nanoparticles is not less than -40 mV. The PSSMA-modified Fe3O4 colloidal nanoparticles are synthesized by a one-step solvothermal method, can be well dispersed in water and various non-aqueous solvents, can realize full-spectrum reversible discoloration under the action of an externally added magnetic field, and are brighter in color. And the preparation method is easy to amplify, and the amplified and synthesized Fe3O4 colloidal photonic crystal can still realize full-spectrum discoloration.

The invention discloses a method for producing astaxanthin by inducing Haematococcus pluvialis, which is characterized by comprising the following steps: in the vegetative growth phase, inoculating Haematococcus pluvialis in a nutrient culture medium, and culturing; continuously keeping irradiation on the Haematococcus pluvialis and ensuring that the intensity of illumination arriving at the Haematococcus pluvialis cell surface is 20-200 mu mol.m<-2>.s<-1>; in the induction stage, adding ethanol and seawater crystal into the production culture medium, wherein the ethanol added into the production culture medium accounts for 0.5-3 vol%, and the seawater crystal added into the production culture medium accounts for 0.5-1 wt%; and continuously introducing filtered air mixed with 1-3 vol% carbon dioxide into the production culture medium, wherein 0.2-0.4L / minute of the air is introduced to every 1L of production culture medium. The method can induce the Haematococcus pluvialis to quickly accumulate the astaxanthin, is simple and has the advantages of high yield and favorable effect.

The invention discloses a method for synthesizing poly-substituted 2-aryl benzothiazoles by utilizing thiourea as a sulphur source. According to the method, thiourea reacts with benzyl chloride to generate an S-benzylisothiourea salt in situ. After that, through the aromatic nucleophilic substitution reaction of the obtained S-benzylisothiourea salt with 2-fluoronitrobenzene and then the one-step reduction (one-pot reaction) process, o-amino phenyl benzyl thioether as an intermediate product can be obtained. Finally, through the iron-catalyzed cross-dehydrogenative-coupling reaction of the o-amino phenyl benzyl thioether, a target product can be obtained. Compared with the traditional synthetic method, the method has the significant advantages of (1) short reaction step, wherein the target product can be synthesized through only three steps of simple reactions by utilizing simple chemical raw materials; (2) mild reaction condition, high atom economy, and relatively safe and cheap reaction reagents; (3) high reaction yield, good substrate tolerance and free of any dangerous or high-toxicity reagent. Therefore, the method might be applied to the large-scale production.

The invention discloses a method for preparing saturated potassium azacyclo-trifluoroborates, which comprises the following steps: 1. reacting pyrrolidine and piperidine used as initial raw materials and tert-butyl carbonic anhydride to generate N-tert-butyl carbonyl pyrrolidine and N-tert-butyl carbonyl piperidine, and performing heating reflux on the N-tert-butyl carbonyl piperazine used as an initial raw material and benzyl chloride to generate N-tert-butyl carbonyl-4-benzyl piperazine; and 2. by means of the orientation effect of the N-tert-butyl carbonyl, in a nitrogen protective atmosphere, reacting with sec-butyl lithium at low temperature, then reacting with trimethylborate to generate saturated o-boric acid, adding a saturated KHF2 solution without separating the boric acid, and performing post treatment to respectively generate a potassium N-tert-butyl carbonyl-2-pyrrolidine trifluoroborate, a potassium N-tert-butyl carbonyl-2-piperidine trifluoroborate and a potassium N-tert-butyl carbonyl-4-benzyl-2-piperazine trifluoroborate. These saturated potassium azacyclo-trifluoroborates prepared by the invention are all white solids, are stable at room temperature in an air atmosphere, belong to very important organic chemical reagents and medical intermediate compounds, and have wide application prospects. The method disclosed by the invention is simple to operate, utilizes the cheap and accessible initial raw materials, achieves high yield and favorable purity, is easy to implement scale-up production, and realizes commercialized production.

The invention provides a preparation method of a nucleic acid probe, which comprises the following steps: mixing and grinding a nucleic acid solid with an amino active group and an activated ester modified dye solid capable of reacting with the amino group, and carrying out one-step reaction to obtain the nucleic acid probe. The nucleic acid solid and the dye solid are mechanically mixed and ground into jelly, and no reaction solvent or other reaction reagent is added in the process. The preparation method of the nucleic acid probe is a solvent-free reaction, does not need a toxic organic solvent, and is more green and pollution-free; and the production process is greatly simplified, and production can be amplified by combining the ball mill.

The invention relates to a key intermediate product compound A for preparing trabectedin and a method for preparing the compound A by using a compound D. According to the method disclosed by the invention, three-step reaction can be continuously operated, and individual separation and purification are not needed, and therefore, an operation is simple and convenient. The invention also relates to amethod for preparing a compound E through reduction of the compound A and application of the intermediate product. The method has the advantages of controllability, simpleness and convenience in operation and stable yield. The formula (1) is shown in the description.

The invention provides an organic modified silver-loaded montmorillonite / polyaniline nano-composite antibacterial agent. A preparation method of the organic modified silver-loaded montmorillonite / polyaniline nano-composite antibacterial agent comprises the following steps: carrying out intercalation modification on montmorillonite by using an organic modifier to prepare organic modified montmorillonite, then adding silver nitrate, conducting stirring, carrying out ultrasonic dispersion to realize uniform dispersion, adding aniline, p-phenylenediamine and acid, and carrying out stirring and reacting at room temperature to obtain the organic modified silver-loaded montmorillonite / polyaniline nano-composite antibacterial agent. The organic modified silver-loaded montmorillonite / polyaniline nano-composite antibacterial agent is prepared by adopting an in-situ polymerization method, silver nitrate can be successfully reduced into nano-silver by utilizing the synergistic effect of aniline and p-phenylenediamine, organic polyaniline is prepared, and the antibacterial performance of the agent is effectively improved.

The invention provides a renaturation and purification method of a recombinant human granulocyte colony stimulating factor. The renaturation and purification method comprises the steps of (1) preparing inclusion body dissolving liquid; (2) adding the inclusion body dissolving liquid to renaturation buffer liquid, adding cystine and cysteine, and performing renaturation; and (3) applying the obtained renaturation liquid to a weak cation chromatography column with a composite ligand for purification. The purification technology is simple and convenient to operate, short in consumption time, andlow in equipment requirement; the cost is saved, and the purification technology is suitable for amplified production scale; and besides, during renaturation, inclusion protein bodies are high in concentration and high in renaturation rate, and after chromatography purification, protein purity is high.

The invention discloses a snuffing type Chinese herbal medicine for preventing influenza. The Chinese herbal medicine is prepared from the following 13 raw herbal materials: cyrtomium rhizome, mint, honeysuckle stem, honeysuckle, radix isatidis, asarum, folia perillae acutae, fructus viticis, herba schizonepetae, lotus leaf, Chinese mosla Herb, eupatorium and borneol. By the Chinese herbal medicine which is not required to be orally taken, the purpose of preventing symptoms such as influenza, wind cold, wind-heat type common cold, initial febrile diseases and drunkenness. When the snuffing type Chinese herbal medicine is used, a user only needs to wear a snuffing type Chinese herbal medicine bag. The snuffing type Chinese herbal medicine has the advantages of good prevention effect, instant effect, effective treatment, no toxic or side effects, low using cost, convenience in use and the like.

The invention relates to a method for degrading beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharides. The method comprises the following steps: introducing an 80-180 mg / mL beta-1,4-glycosidic-bond-containing high-molecular-weight polysaccharide solution into an electrolyzer provided with a cathode and an anode, regulating the solution to acidity, introducing 4.0-6.0L of oxygen to the cathode for every liter of the polysaccharide solution, and introducing a direct current while controlling the current density of the cathode at 10-13 mA / cm<2>; and under such conditions, carrying out degradation, and collecting the electrolyte. The method has favorable selectivity for beta-1,4-glycosidic-bond-containing polysaccharides, has the advantages of simple technical operation, time saving, high efficiency, mild reaction conditions and low environmental pollution, and is beneficial to scale-up production. More importantly, compared with the acid-process degradation and hydrogen peroxide degradation, the electro-Fenton process has higher degradation rate for polysaccharides, and the molecular weight range of the obtained low-molecular-weight polysaccharides is narrower.

The invention discloses a porcine parvovirus oral vaccine composition which includes the components a) and b), is basically composed of the a) and b), or is composed of the a) and b), wherein the component a) is viroid particles formed by self-assembling porcine parvovirus VP2 protein expressed by Kluyveromyces marxianus recombinant engineering bacteria; b) pharmaceutically or veterinarily acceptably auxiliary material for oral-taking preparations. The invention also discloses a preparation method and an application of the porcine parvovirus oral vaccine composition. The porcine parvovirus oral vaccine composition has excellent immunogenicity. An orally immunized mouse can generate protective antibodies which have high titer. The oral vaccine composition has high safety and low productioncost, can be produced to an amplified scale and has simple operation, and can be used for preventing and alleviating clinical symptoms related to PPV infection.

The invention discloses a preparation method of a niraparib intermediate that is (S)-3-(4-bromophenyl) piperidine, which comprises the following steps: by using chiral 1-phenylethylamine as a raw material, carrying out alkylation, condensation, cyclization and reduction, and finally removing an amino protection group to obtain the (S)-3-(4-bromophenyl) piperidine. The preparation method of the (S)-3-(4-bromophenyl) piperidine has the advantages of short route, few steps, simple and convenient process operation, avoidance of use of dangerous reagents, highly toxic reagents and irritant malodorous reagents, reduction of potential safety hazards to operators, reduction of the operation safety level of production, environmental protection, and facilitation of industrialization.