49results about How to "Good experimental basis" patented technology

The invention provides the construction of a recombinant lentiviral vector targeting farnesyl pyrophosphate synthase RNA interference. The most effective target sequence of FDS gene RNAi is screened out in the tool cell 293T cells, and the double-stranded DNA of the most effective target sequence is synthesized and connected to pGCSIL - GFP vector, the recombinant vector was successfully constructed through restriction sequencing. Studies have shown that the constructed RNA interference vector LV-sh-FDS can down-regulate the expression of FDS mRNA level in neonatal rat cardiomyocytes, and can also down-regulate the expression of cardiac hypertrophy markers such as cell area and marker genes beta-MHC and BNP. While down-regulating FDS, it can also effectively inhibit the activity of RhoA, and can be used in the preparation of drugs for treating cardiac hypertrophy diseases. It can also be used in the preparation of drugs for regulating cholesterol metabolism.

A method for constructing multifunctional gene therapy vectors based on gold nanoparticles. The invention belongs to the technical field of CT contrast agents and non-viral gene carriers, and specifically relates to gold nanoparticles as the skeleton, including spherical gold nanoparticles, rod-shaped gold nanoparticles (with different axis-to-diameter ratios), arrowhead-shaped gold nanoparticles, and octahedral gold nanoparticles. The morphology of gold nanoparticles, etc., using modified bovine serum albumin to wrap gold nanoparticles, and ATRP method to construct a multifunctional gene carrier. The polymerization reaction is stable and easy to control, and a variety of high-performance cationic gene carriers with different monomer contents can be prepared according to needs, and the storage stability is good, and the original performance can be maintained after being placed for several days or months. The cationic gene carrier has a higher transfection efficiency than the international "gold standard" PEI in HepG2, COS7, 293, C6 and other cells, and its cytotoxicity is much lower than that of PEI. It is simple to use and has commercial potential. And the material has a good CT imaging effect, which lays a good experimental foundation for the development of new contrast agents.

The invention relates to a recombinant lentivirus-based vector for implementing the RNA (Ribose Nucleic Acid) interference aiming at an FLG (filaggrin) gene and preparation of the recombinant lentivirus-based vector. A lentivirus-based vector of shRNA (shorthairpin RNA) aiming at the FLG gene is constructed through experiments; a synthesized DNA fragment aiming at the ShRNA is mediated by the lentivirus-based vector; 293T cells are subjected to co-transfection together by the lentivirus-based vector and two vectors pHelper1.0 and pHelper2.0 to carry out culture; and after the recombinant lentivirus-based vector is obtained, target cells are subjected to transfection so as to implement the RNA interference aiming at the FLG gene. The adopted self-inactivated third-generation lentivirus-based vector has the advantages of safety, reliability, capability of infecting nondividing cells, long-term expression of integrating a target gene to a target cell gene, small immune response and the like and is an ideal vector. The lentivirus-based vector disclosed by the invention has the interference effect reaching 75 to 95 percent on normal skin cells HACAT of people, and thus, the recombinant lentivirus-based vector disclosed by the invention lays a good experimental foundation for further research related to the FLG gene and can be widely used for the in-vivo gene therapy and the gene functional research.

The invention relates to a graphene / metal / MgB2 composite material with high intergranular connectivity and a preparation method thereof. Mg, B and a certain proportion of graphene and metal are jointly subjected to ball milling, and finally, an MgB2 superconducting block body is obtained through high-temperature sintering. The MgB2 superconducting block body prepared through the method is good in intergranular connectivity, thin and small in grain and even in distribution, and forms an eutectic liquid phase with the Mg at the low temperature through doping of Cu or Ni, favorable conditions are provided for diffusion and reaction between Mg and B particles, the reaction free energy is reduced, and the formation temperature of MgB2 is decreased to 500 DEG C. A reaction site is further provided for the particles through adding of the graphene, and thus MgB2 particles are subjected to nucleation and growth on a graphene carrier, so that the MgB2 forms a network structure, and the intergranular connectivity is improved. The used raw materials are easy to obtain, the material preparation method is mature in development, operation is convenient, and the process is controllable; and according to the method, the MgB2 reaction temperature is effectively decreased, and the intergranular connectivity of the MgB2 is improved.

The invention relates to a CT / MR / near infrared optical three-mode targeted contrast agent based on the lung non-cellule cancer as well as a preparation method and application thereof. The preparation method comprises the following steps: firstly, modifying the amino terminal of a fifth-generation polyamide-amine dendrimer with DOTA-NHS; secondly, connecting folic acid to the dendrimer by polyethylene glycol; thirdly, further modifying with Cy5.5; fourthly, preparing gold nanoparticles by using the modified dendrimer as a template through sodium borohydride reduction, and chelating gadolinium ions to the dendrimer; fifthly, carrying out complete acetylization on amino remained on the surface of the dendrimer; and finally, dialyzing the reacted solution, lyophilizing to obtain a final product, and evaluating in-vivo and in-vitro CT / MR / near infrared optical three-mode contrast performance of the product. The method is simple and feasible, has excellent in-vivo and in-vitro lung non-cellule cancer cell target effect, and has a potential application prospect of targeted CT / MR / near infrared optical contrast.