Photoresponse silicon oxide nanometer vector and preparation method thereof

A light-responsive, nano-carrier technology, applied in the field of multifunctional nano-carriers constructed by atom transfer radical polymerization and its preparation, can solve the effects of difficult to achieve cancer treatment effects, lack of precise control, and tumor heterogeneity and other issues, to achieve the effect of improving gene transfection efficiency, low price, and controllable size

Active Publication Date: 2018-01-12
MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, it is difficult for a single gene therapy method to achieve the ideal cancer treatment effect. Combining multiple cancer treatment methods to exert a synergistic anti-cancer effect has become the mainstream trend of cancer treatment research. Great potential in cancer treatment
In the existing chemotherapy / gene and gene / PDT combined therapy systems, gene release mainly depends on the difference between the tumor microenvironment and normal tissue cells, making it susceptible to tumor heterogeneity, and lacks precise control, which limits Chemo / gene, gene / PDT therapy in a wide variety of different cancers

Method used

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  • Photoresponse silicon oxide nanometer vector and preparation method thereof
  • Photoresponse silicon oxide nanometer vector and preparation method thereof
  • Photoresponse silicon oxide nanometer vector and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Embodiment 1: Preparation of hollow silica nanoparticles HNP

[0043] 0.2g cetyltrimethylammonium bromide (CTAB) was dissolved in 96mL H 2 O, add 0.055g perfluorooctanoic acid (PFOA), then add 0.7mL NaOH (2M), and finally add 1mL tetraethyl orthosilicate (TEOS), and stir vigorously at 80°C for 2h. The resulting reaction product was collected by centrifugation, dried at 60°C, and calcined at 550°C for 6h to remove the template.

Embodiment 2

[0044] Example 2: Preparation of photosensitive group functionalized silica nanoparticles HNP-Cou

[0045] Dissolve 1.12g of 1,3-benzenediol (10.18mmol) and 1.11g of ethyl bromoacetoacetate (8.52mmol) in 70% H 2 SO 4 , reacted at room temperature for 8 hours, added 72 mL of water, filtered, and dried in vacuo to obtain the photosensitive group 4-bromomethyl-7-hydroxy-coumarin (Cou).

[0046] Take 200 mg of silicon oxide nanoparticles obtained in Example 1 and dissolve them in 18 mL of absolute ethanol, add 2 mL of water and 300 μL of 3-aminopropyltriethoxysilane (APTES), react at room temperature for 6 hours, add 160 μL of triethylamine, and continue the reaction for 12 hours . The obtained product was centrifuged and washed three times with ethanol, dried in vacuum, and solidified at 130°C for 3 hours to obtain HNP-NH 2 .

[0047] 1 g of succinic anhydride (SA) was dissolved in 10 mL of anhydrous DMF. The above obtained HNP-NH 2 Dissolve 190 mg in 10 mL of anhydrous DMF...

Embodiment 3

[0050] Embodiment 3: the preparation of silica nanoparticle initiator HNP-Cou-Br

[0051] Get the photosensitive group functionalized silicon oxide nanoparticles HNP-Cou100mg and 105mg 2-bromoisobutyric acid (0.63mmol) obtained in Example 2 and dissolve them in 10mL of anhydrous DMF, then add 105mg of potassium carbonate (K 2 CO3 ) (0.63mmol), reacted at 37°C for 24h. The product was centrifuged three times with methanol and water, and dried in vacuum to obtain HNP-Cou-Br.

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Abstract

The invention belongs to the technical field of a non-viral gene vector and a medicine/photosencitizer vector, and particularly relates to a multifunctional nanometer vector and a preparation method of the multifunctional nanometer vector, wherein the multifunctional nanometer vector is built by using silicon oxide nanometer particles as substrates, functionalizing the silicon oxide nanometer particles through surface modification, introducing photosensitive groups with ultraviolet/near infrared light response and using an ATRP (Atom Transfer Radical Polymerization) method. The multifunctionalnanometer vector has higher transfection efficiency than international JINBIAO PEI in cells of HepG2, COS7, 293, C6, Hela and the like, and has photoresponse gene release performance. Under the photostimulation, the photosensitive groups on the surfaces of the silicon oxide nanometer particles perform photolysis, so that positive ions fall off, and the genes are released; and the gene transfection efficiency can be greatly improved. Various different medicine or photosencitizer molecules can be loaded into porous structures of mesoporous silicon oxide nanometer particles; and good experimentfoundation is laid for the development of the novel photoresponse nanometer vector. The method has the advantages that the use method is simple; and the commercialization potential is realized.

Description

(1) Technical field [0001] The invention belongs to the technical field of non-viral gene carrier and drug / photosensitizer carrier, and specifically relates to the use of silicon oxide nanoparticles as a substrate, the use of surface-modified functionalized silicon oxide nanoparticles, and the introduction of photosensitive groups with ultraviolet / near-infrared photoresponses. A multifunctional nanocarrier constructed by atom transfer radical polymerization (ATRP) and a preparation method thereof. (2) Background technology [0002] In recent years, malignant tumors have become the main killer that threatens human life and health, and the treatment of malignant tumors has become a worldwide problem that needs to be solved urgently, which has extremely important strategic significance. Due to the influence of many adverse factors, such as recurrence after surgery, strong toxic and side effects of radiotherapy and chemotherapy, the commonly used tumor treatment methods in clini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K41/00A61K47/04A61K47/32A61K45/06A61P35/00C07D311/16C01B33/18B82Y30/00B82Y40/00
Inventor 刘景丰刘小龙林心怡曾永毅
Owner MENGCHAO HEPATOBILIARY HOSPITAL OF FUJIAN MEDICAL UNIV
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