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Preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in targeted manner

A technology of nanofibers, hyaluronic acid, applied in the direction of biochemical equipment and methods, fiber types, fiber processing, etc.

Inactive Publication Date: 2014-04-23
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] Retrieval of relevant literature and patents at home and abroad shows that the preparation of hyaluronic acid-functionalized nanofibers for targeted capture of cancer cells has not been reported yet

Method used

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  • Preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in targeted manner
  • Preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in targeted manner
  • Preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in targeted manner

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Weigh 9.68732g of PVA, dissolve it in 71.04g of ultrapure water, stir slowly with a glass rod for about 30 minutes until the PVA powder is completely swollen, then place it on a magnetic stirrer and heat and stir in a water bath at 80°C for 3 hours to obtain a uniform concentration of 12wt% PVA solution.

[0066] Take by weighing the PVA spinning solution of 12wt% that 15g has configured, wherein the amount of PVA is 1.8g, according to the mass ratio of PVA and PEI solid in the mixed solution of setting is 3:1, thereby draw the quality of required PEI as 0.6g, weigh 0.6g PEI and add it to 15g12wt% PVA spinning solution, continue to add 4.4g water, mix it with a vortex mixer, then use ultrasound for 30min to disperse it evenly, stir it overnight with a magnetic force, and get the spinning solution PVA / PEI The total mass percentage concentration is 12wt%.

[0067] The spinning process conditions used are: the spinning voltage is 18.6kV, of which the positive pressure is ...

Embodiment 2

[0070] Put the PVA / PEI nanofiber mat prepared in Example 1 above the petri dish filled with 25% glutaraldehyde solution, put the two together in a desiccator, and vacuumize and crosslink for 18 hours. The appearance of the obtained PVA / PEI nanofibers was pink. After soaking the glutaraldehyde-crosslinked nanofibers in the aqueous solution for 7 days, the SEM results showed that the fiber morphology remained good, there was no adhesion phenomenon, and the average fiber diameter increased to 660.8± 95.7nm (with figure 1 b), showing that the fiber has good water stability.

Embodiment 3

[0072] The mass of PVA / PEI nanofibers was weighed to be 120.4 mg, and the mass ratio of PVA:PEI=3:1 was calculated to obtain that the mass of PEI was 30.1 mg. According to the primary amine (R-NH 2 ) 1.08mol, secondary amine (R 2 -NH) 1.49mol, tertiary amine (R 3 -N) 1 mol, of which only primary and secondary amines can participate in the amidation reaction. Therefore, in the 120.4 mg fiber membrane, the amino group that can participate in the amidation reaction is 0.563 mol. Based on the molar ratio of carboxyl:amino = 1:1, the required HA is 0.563mol and the mass is 213.94mg. Calculated based on the molar ratio HA:EDC:NHS=1:5:5, the amount of EDC is 539.64 mg, and the amount of NHS is 323.98 mg, which are respectively dissolved in 10 mL of water and slowly added dropwise to 10 mL of aqueous solution containing 213.94 mg of HA, thereby The activation reaction was carried out with a reaction system with a total volume of 30 mL for 3 h, 30 mL of HA reaction solution was add...

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Abstract

The invention relates to a preparation method for hyaluronic acid functionalized nano fibers applied to capturing cancer cells in a targeted manner. The preparation method comprises the following steps: (1) by taking water as a solvent, preparing a PVA / PEI (Poly(Vinyl Alcohol) / Polyethylenimine) spinning solution and carrying out electrostatic spinning; drying and carrying out vacuum cross-linking treatment in a dyer containing a glutaraldehyde solution to obtain PVA / PEI nano fibers with good water stability; (2) adding activated hyaluronic acid into the PVA / PEI nano fibers with the good water stability; reacting on a shaking bed to obtain electrostatic spinning PVA / PEI nano fibers which are functionally modified by the hyaluronic acid; then adding triethylamine and acetic anhydride to carry out acetylation treatment on all residual amino groups on the surface of a PVA / PEI nano fiber film; and finally, washing and drying to obtain the product. The preparation method provided by the invention is low in raw material price and simple in preparation process; the prepared hyaluronic acid functionalized electrostatic spinning nano fibers have good appearance, structural stability, biocompatibility and blood compatibility and the application prospect is wide.

Description

technical field [0001] The invention belongs to the field of preparation of nanomaterials for targeted capture of cancer cells, in particular to a method for preparing hyaluronic acid functionalized nanofibers for targeted capture of cancer cells. Background technique [0002] Nanofiber material is a new type of material and a cutting-edge technology in the field of nanoscience. Among them, polymer nanofibers are an important research direction and have a wide range of application values. Especially through some surface modification, the polymer nanofiber material has dual properties, which has attracted widespread attention from scientists and will have broad application prospects in the future. [0003] Electrospinning technology has obvious advantages in the preparation of nanofibers. Using electrospinning technology can reduce the cost, and the manufacturing process is simple and convenient. And the prepared nanofiber has large specific surface area and high porosity....

Claims

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Application Information

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IPC IPC(8): D06M15/03D06M13/328D06M13/188D06M13/123D01F8/10D01F8/16C12Q1/02D06M101/24D06M101/30
Inventor 史向阳赵毅丽范章余王哲
Owner DONGHUA UNIV
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