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Fermentation medium for efficiently producing short branched fatty acid and fermentation process

A technology of fermentation medium and fermentation process, applied in the direction of fermentation, microbe-based methods, microbes, etc., to achieve the effects of easy promotion, simple production process, and improved economic benefits

Inactive Publication Date: 2019-06-21
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the fermentation of short-chain branched-chain fatty acids by Bacillus using these cheap and readily available waste biological resources

Method used

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  • Fermentation medium for efficiently producing short branched fatty acid and fermentation process
  • Fermentation medium for efficiently producing short branched fatty acid and fermentation process
  • Fermentation medium for efficiently producing short branched fatty acid and fermentation process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] In this example, Bacillus licheniformis DW2 was used as the starting strain, and a large number of experiments were conducted to verify that the combination of medium composition and culture conditions can achieve efficient production of short-chain branched-chain fatty acids.

[0046] The Bacillus licheniformis DW2 has a deposit number of CCTCC No: M2011344.

[0047] The composition of the seed medium used in this example: 10g / L peptone; 5g / L yeast extract powder; 10g / L sodium chloride; pH 7.0-7.2; solid medium plus agar 18g / L.

[0048] The detection method and quantitative method of short-chain branched-chain fatty acids in this example: after Bacillus licheniformis was fermented in the short-chain branched-chain fatty acid fermentation medium for 48 hours, the fermentation liquid was centrifuged to remove the bacteria, and the supernatant was collected, and 1 mL of the centrifuged supernatant was taken , and add an equal volume of n-butanol, with n-butanol as the int...

Embodiment 2

[0091] In this example, the waste rapeseed cake was used as the organic nitrogen source, and the efficient production of short-chain branched-chain fatty acids was realized through the combination of specific medium composition and culture conditions

[0092] (1) Starting strain: Bacillus licheniformis DW2;

[0093] (2) Seed cultivation:

[0094] Seed medium: 10g / L peptone; 5g / L yeast extract powder; 10g / L sodium chloride; pH 7.0-7.2; solid medium plus agar 18g / L;

[0095] Seed cultivation: The cultivation temperature is 37°C, the liquid volume in the 250mL Erlenmeyer flask is 50mL, the shaker speed is 240r / min, and the cultivation time is 10h;

[0096] (3) Fermentation culture

[0097] Fermentation medium composition: corn starch 45g / L, rapeseed meal 80g / L, NH 4 Cl 2g / L, CaCO 3 6g / L, K 2 HPO 4 1g / L, MgSO 4 1g / L, MnSO4 0.05g / L, the culture medium was made to volume with deionized water.

[0098] Fermentation culture conditions: culture temperature 37°C, shaking tabl...

Embodiment 3

[0103] In this example, waste cottonseed cake and rapeseed cake are used as organic nitrogen sources, and the efficient production of short-chain branched fatty acids is realized through the combination of specific medium composition and culture conditions

[0104] (1) Starting strain: Bacillus licheniformis DW2;

[0105] (2) Seed cultivation:

[0106] Seed medium: 10g / L peptone; 5g / L yeast extract powder; 10g / L sodium chloride; pH 7.0-7.2; solid medium plus agar 18g / L;

[0107] Seed cultivation: The cultivation temperature is 37°C, the liquid volume in the 250mL Erlenmeyer flask is 50mL, the shaker speed is 240r / min, and the cultivation time is 10h;

[0108] (3) Fermentation culture

[0109] Fermentation medium composition: corn starch 45g / L, cottonseed meal 80g / L, NH 4 Cl 2g / L, CaCO 3 6g / L, K 2 HPO 4 1g / L, MgSO 4 1g / L, MnSO4 0.05g / L, the culture medium was made to volume with deionized water.

[0110] Fermentation culture conditions: culture temperature 37°C, shak...

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Abstract

The invention provides a bacillus fermentation medium for efficiently producing short branched fatty acid by waste cakes and bean dreg resources and a fermentation process. Efficient production of theshort branched fatty acid is achieved by matching specific medium compositions with culture conditions. A fermentation medium formula comprises, 10-100g / L of corn starch, 20-100g / L of waste organic nitrogen sources, 1-10g / L of ammonia chloride, 3-15g / L of calcium carbonate, 1-10g / L of dipotassium phosphate, 1-10g / L of magnesium sulfate, 0.005-0.10g / L of manganese sulfate and pH (potential of hydrogen) is 6-8. Fermentation culture conditions include that culture temperature is 25-40 DEG C, the rotating speed of a shaking table is 180-240r / min, and fermentation time is 30-60 hours. According tothe fermentation medium, low-cost soybean residue, rape seed cakes and cottonseed cakes serve as fermentation raw materials. Under the process conditions, the yield of the short branched fatty acid is increased by fermentation verification of geobacillus, bacillus subtilis and bacillus amyloliquefaciens. The process has the advantages that the process is simple in assembly, environmentally friendly, economical, high in yield, stable and wide in applicable range. The fermentation medium has a certain application prospect.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a fermentation medium and a fermentation process for bacillus to efficiently produce short-chain branched-chain fatty acids by using waste cake and bean dregs resources, which has high stability and wide application range, and can realize short-chain branched-chain fatty acids economical and efficient production. Background technique [0002] Short branched chain fatty acids (SCFAs) are a class of carboxylic acids with 4-6 carbon atoms and have methyl branches on one or two carbons. They and their derivatives are versatile platform chemicals with high market potential for use in manufacturing, pharmaceutical and food industries. For example, isobutyric acid (IBA) can be used in the production of plasticizers, surfactants, textile auxiliaries, fragrances and flavorings. IBA is also used in the synthesis of methacrylic acid, an industrially important monomer for ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/40C12R1/125C12R1/07C12R1/10
Inventor 陈守文石姣占杨杨王欢许勇周梦林马昕王勤
Owner HUBEI UNIV
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