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60results about How to "Efficient extraction method" patented technology

Method for separating tungsten and molybdenum from molybdenum-contained phosphotungstic acid/phosphowolframate solution

ActiveCN105349803AAvoid large acid lossesEfficient extractionAmmonium hydroxideDecomposition
The invention relates to a method for separating tungsten and molybdenum from a molybdenum-contained phosphotungstic acid / phosphowolframate solution. The method comprises the following steps that 1, hydrogen peroxide is added into the molybdenum-contained phosphotungstic acid / phosphowolframate solution; 2, a reduction or decomposition reaction is performed for precipitation to obtain tungstic acid, and filtering and washing are performed; 3, further roasting is performed on the obtained tungstic acid to obtain tungsten trioxide, or the tungstic acid is dissolved through ammonium hydroxide to obtain an ammonium tungstate solution, and finally evaporatve crystallization is performed to obtain APT; 4, an extraction agent is adopted for extracting molybdenum in the filtrate, a raffinate is returned back to the phosphotungstic acid / phosphowolframate solution; and 5, reverse extraction with ammonium hydroxide is performed to obtain an ammonium molybdate solution, and acid precipitation is performed to obtain ammonium tetramolybdate. The molybdenum separated out through the method is directly used for preparing the product, the separation effect is good, the operation process is simple and easy to control, and industrial application and popularization are easy.
Owner:CENT SOUTH UNIV

Highly effective extraction method for magnolol and honokiol crude extract

The invention discloses a method for extracting magnolol and honokiol crude extract with high efficiency, the method is that: the magnolol is dried up and crushed and sieved into 30 mesh, the magnolol is added to edible alcohol twice at the weight proportion of one grams of magnolol powder to 12 to 20ml edible alcohol, the extract temperature is 80 to 90 DEGC, the extract time is 2.0 to 3.0 hours each time, the concentration of edible alcohol is 50 to 70 percent, the two extraction crude filter liquors are combined and sieved into 200-mesh nylon fabric, a high speed centrifuge is used for centrifuging and clarifying and a clear liquid is taken out; the clear liquid at the temperature of 40 to 45 DEG C is carried out vacuum concentration by a rotary evaporator, the edible alcohol solvent is recovered and is frozen and dried in vacuum so as to obtain the crude extract, the extract rate of magnolol and honokiol is obviously higher than that of the prior art, the amounted manufacturing cost is relatively low. The method is harmless to the human body as well as to the environment by adopting edible alcohol for extraction; therefore, the method is particularly safe.
Owner:HUNAN AGRICULTURAL UNIV

Method for detecting pear endogenous hormone by using ultra-high performance liquid chromatography electrospray tandem mass spectrum

The invention discloses a method for detecting a pear endogenous hormone by using an ultra-high performance liquid chromatography electrospray tandem mass spectrum. The method comprises the following steps of: crushing obtained pear tissues, then adding an extracting solution for extraction, after ending extraction, adding an extraction reagent to a system, and extracting to obtain a crude extract of the endogenous hormone; separating and purifying the crude extract by using a solid-phase extraction column to obtain a detection sample; analyzing a detection sample by using the ultra-high performance liquid chromatography electrospray tandem mass spectrum to obtain the variety and the content of the endogenous hormone in the pear tissues, wherein the extracting solution comprises isopropanol, water and hydrochloric acid, and the volume ratio of the isopropanol, the water and the hydrochloric acid is (1.5-2.5):1:(0.001-0.002), and the extraction agent is dichloromethane. The method has the advantages of high sensitivity, simple pretreatment, high recovery rate, high degree of accuracy and degree of precision and the like and is used for detecting and confirming the endogenous hormones in multiple plant issues.
Owner:ZHEJIANG UNIV

Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity, enzymolysis preparation method and application thereof

InactiveCN104131059AImprove bioactive availabilityEfficient enzymatic hydrolysis abilityPeptide preparation methodsFermentationAlgluceraseChemistry
The invention discloses an enzymolysis preparation method of Cannabis sativa seed meal polypeptide with alpha-glucosidase inhibiting activity. The method includes: drying Cannabis sativa seed meal and crushing it into powder, adding water and stirring the mixture evenly, adjusting the pH to 8.0-10.5, adjusting the temperature to 30-80DEG C, performing stirring at a uniform speed for 0.5h-2h, conducting centrifugation and adjusting the pH of the supernatant to 3-6, thus obtaining protein precipitate; adding water to dissolve the protein, with the proportion being 2-7g:100mL, adjusting the pH to 7.0- 9.5, then adding alkaline protease, with the ratio of the protein weight to the enzyme activity being 0.1-5:1000, keeping the temperature at 50-75DEG C, and controlling the time at 1-4h; subjecting the enzymatic hydrolysate to separation and purification through macroporous adsorption resin and a Sephadex column, and carrying out collecting and drying, thus obtaining the alpha-glucosidase inhibiting active peptide powder. The Cannabis sativa seed meal polypeptide provided by the invention adds new sources for alpha-glucosidase inhibiting active substances, realizes full utilization of raw materials, and has the advantages of high product activity, convenient and feasible preparation process, and environmental protection, etc.
Owner:SOUTH CHINA UNIV OF TECH

Index keyword extraction method and system based on big geological data

The invention relates to an index keyword extraction method and system based on big geological data. The method comprises the following steps of: importing document and performing geological data format conversion, sentence division, word characteristic tagging and position marking; adding a weight coefficient to the segmented document; initially mining and extracting keywords by a keyword mining algorithm; performing weighted operation on the extracted keyword phrases to obtain the comprehensive weight value of each phrase; initially screening according to the sizes of the comprehensive weight values, and reducing the number of the phrases to be determined; matching the phrases with a word library, and finding terms matched with the phrases or relatively-stipulated terms in the word library; performing association degree calculation on the terms and the document, determining the size of the association degree of the terms, and screening again according to the sizes of the association degree; and finally, sequencing the terms according to industrial characteristics, height correlation, features and the like, and determining the order of the keywords. According to the method, file background and relevance can be considered, a vague keyword is abandoned to the greatest extent, the keywords comprehensively reflecting the core content of the document are provided and are sequenced according to a certain logic order, thus, document retrieval is achieved, convenience is provided for the improvement of retrieval efficiency, and the method is a high-efficient index keyword extraction method for the big geological data.
Owner:CHENGDU UNIVERSITY OF TECHNOLOGY

Method for extracting tungsten from phosphotungstic acid/phosphotungstate solution

The invention relates to a method for extracting tungsten from a phosphotungstic acid / phosphotungstate solution. The method comprises following steps: (1), hydrogen peroxide is added to the phosphotungstic acid / phosphotungstate solution, and a mixed solution containing peroxo-tungstic acid is obtained; (2), tungstic acid is precipitated with a reduction reaction or a decomposition reaction, filtered and washed, and a tungstic acid precipitate and a filtrate are obtained; (3), tungstic acid is calcined, and tungsten trioxide is prepared; or, tungstic acid is dissolved in ammonia water, an ammonium tungstate solution is prepared, and APT (ammonium paratungstate) is prepared through evaporative crystallization; (4), the filtrate returns to the phosphotungstic acid / phosphotungstate solution for recycling. According to the method, existence forms and characteristics of various species in a mixed acid solution system are taken into consideration, and tungsten obtained through extraction can be directly used for preparing tungsten products; the method has the advantages of good separation effect, simple and easily controlled operation process and easiness in industrial popularization and application.
Owner:CENT SOUTH UNIV

Viral nucleic acid extraction kit and extraction method

The invention discloses a virus nucleic acid extraction kit. The virus nucleic acid extraction kit comprises a lysis solution, a first rinsing solution, a second rinsing solution, an elution solution,protease K and magnetic beads; the lysis solution comprises sodium perchlorate with the concentration of 1.0-5.0 mol / L, Tris-HCl with the concentration of 10-200 mmol / L, EDTA with the concentration of 1-50 mmol / L, and Triton X-100 with the concentration of 50-100 microlitre / milliliter; the first rinsing solution is sodium perchlorate solution with the concentration of 2.0-2.5 mol / L, and the solvent of the sodium perchlorate solution is 70-80% ethyl alcohol; the second rinsing solution comprises DEPC water and absolute ethyl alcohol, wherein the volume ratio of the DEPC water to the absolute ethyl alcohol is (3-4):(6-7); the elution solution is Tris-HCl-DEPC water solution with the concentration of 8-12 mmol / L. The invention further discloses a method for extracting viral nucleic acid by using the extraction kit. Through optimization of the reagent formula, the extraction kit has the advantages of being rapid and efficient and avoiding pollution, a large amount of time and reagent canbe saved, and the extraction efficiency of the virus nucleic acid is improved.
Owner:GUANGZHOU YIXIN BIOTECH CO LTD

Pig feed additive for improving intestinal health and boosting immunity

The invention discloses a pig feed additive for improving intestinal health and boosting immunity and belongs to the field of feed additives. The additive is prepared from compound plant polysaccharide, complex enzyme preparation, lactobacillus plantarum powder, lactobacillus buchneri powder, stevia rebaudiana powder, fructus chebulae powder, orange peel powder, arginine, glutamine dipeptide, alpha-oxoglutarate, cystine, taurine and a carrier, wherein corncob powder and peanut shell powder serve as the carrier. By the adoption of the additive, pig intestinal health can be improved, intestine development of live pigs can be promoted, the feed digestibility and absorptivity of live pigs can be improved, pig growth can be promoted, marketing period can be shortened, economical benefits can be increased, and feeding effect can be improved. Meanwhile, the immunity of pigs can be improved, the morbidity of pigs can be reduced, and application prospects are quite broad.
Owner:YUNNAN TWINS FEED CO LTD

Extraction method for extraction and pharmaceutical containing the same

The invention discloses an extracting method of mezerein and drug with mezerein, which comprises the following steps: using alcohol to extract gewang; condensing; extracting; chromatographing; eluting; recrystallizing; drying; obtaining the product with receiving rate at 0.014%-0.03% and purity at 97.00%-99.99%. The drug with mezerein is made of 45-55% mezerein, 40-50% microcrystal cellulose and 3-7% magnesium stearate.
Owner:HARBIN MEDICAL UNIVERSITY

Method for preparing collagen through high-pressure enzymolysis

The invention discloses a method for preparing collagen through high-pressure enzymolysis, and belongs to the technical field of bioengineering. The method comprises the following steps: dispersing smashed animal skin, bone and scale and a feed liquid into an enzyme solution according to the proportion of 1:(1 to 15), adding a buffer solution, adjusting the pH to be 4-9, conducting enzymolysis at the temperature of 30-70 DEG C and the pressure of 100-700 Mpa, and conducting solid-liquid separation on the enzymolysis product so as to obtain a collagen liquid; conducting concentration and drying on the collagen liquid to obtain collagen powder. According to the extracted collagen, the property and the activity of natural collagen are kept to the maximum limit, and compared with the prior art, the collagen extraction rate is obviously improved, and the enzymolysis time is obviously shortened (as low as 0.1 h), so that the efficiency is improved, the energy consumption is reduced, pollution on the environment in the extraction process is also avoided, and the extraction method is efficient, economical and green.
Owner:杨德庆 +1

Method for extracting spica prunellae and rosmarinus officinalis acid on basis of response surface processes

The invention belongs to the technical field of spica prunellae extraction, and particularly discloses a method for extracting spica prunellae and rosmarinus officinalis acid on the basis of responsesurface processes. The method has the advantages that spica prunellae is extracted by the aid of cellulase enzymatic hydrolysis processes, technological parameters such as the enzymatic hydrolysis temperatures, the enzymatic hydrolysis pH (potential of hydrogen) values, the cellulase usage and the enzymatic hydrolysis time are reasonably determined and selected in extraction procedures, accordingly, the yield of the spica prunellae and rosmarinus officinalis acid can reach 17.69 mg / g at least, toxic organic solvents can be effectively omitted, the purpose of efficiently extracting the spica prunellae and rosmarinus officinalis acid can be achieved, and the method is high in efficiency and is controllable and stable.
Owner:TONGREN POLYTECHNIC COLLEGE

Extraction and purification method of dioscin

InactiveCN108148107AAvoid preprocessingFull of active ingredientsSteroidsPurification methodsOrganic solvent
The invention discloses an extraction and purification method of dioscin. The extraction and purification method comprises the following steps of: after carrying out multiple circulating extraction treatment on a mixture of dioscoreaceae rhizome powder and organic solvents, filtering to obtain dioscin extracting solution, wherein each extraction treatment comprises microwave treatment and refluxing-method treatment which are carried out in sequence; purifying the dioscin extracting solution to obtain dioscin. The extraction and purification method disclosed by the invention has the beneficialeffects that the technical operation is simple, the use amount of the organic solvent is greatly reduced, the process time is shortened by 50% or more, acid and alkaline are not used, and the main organic solvents can be recycled respectively, so that the generation amount of 'three wastes' (waste water, waste gas and waste solid) after industrial production is greatly reduced.
Owner:贯虹科技有限公司

Extraction method of selenoprotein in cardamine violifolia, selenoprotein obtained by extraction method and application of selenoprotein

The invention provides an extraction method of selenoprotein in cardamine violifolia, the selenoprotein obtained by the extraction method and application of the selenoprotein. The extraction method comprises the following steps of carrying out enzymolysis on the cardamine violifolia by adopting enzymes including cellulase to obtain a cardamine violifolia enzymatic hydrolysate; and concentrating the cardamine violifolia enzymatic hydrolysate, and drying the concentrated cardamine violifolia enzymatic hydrolysate. According to the method disclosed by the invention, the selenoprotein in the cardamine violifolia is extracted through enzymolysis of the enzymes including the cellulase, so that nutritional ingredients in the cardamine violifolia are not damaged, the efficiency of extracting the selenoprotein through enzymolysis is high, the yield of the selenoprotein is relatively high, the protein content in the selenoprotein is high, the content of total selenium is high, and the total selenium is organic selenium; and in addition, the extraction method is simple, efficient, low in cost, environmentally friendly and easy for large-scale industrial production.
Owner:ENSHI QINGJIANG BIO ENG

Dendrobium polysaccharide extract, and preparation method thereof

InactiveCN110156905AEfficient extraction methodThe extraction method is stablePre treatmentDendrobium
The invention relates to a dendrobium polysaccharide extract, and a preparation method thereof. The preparation method comprises following steps: 1, pretreatment, wherein dendrobium raw material is prepared, and is crushed so as to obtain a raw material powder; 2, extraction, wherein the raw material powder is added into water so as to obtain a pre-mixed solution, the pre-mixed solution is subjected to high differential pressure continuous extraction, an obtained extracted solution is collected, the extraction pressure of high differential pressure continuous extraction is controlled to be -0.1 to 50MPa; 3, separation, wherein the extracted solution is subjected to centrifugation, an obtained supernatant is collected and is subjected to microfiltration so as to obtain a microfiltered clearliquid; and 4, condensation, wherein the microfiltered clear liquid is subjected to condensation so as to obtain the dendrobium polysaccharide extract. The preparation method is capable of increasingdendrobium polysaccharide yield with ensured dendrobium extraction efficiency, and is capable of realizing industrialized continous large scale production.
Owner:广州泽力医药科技有限公司

Extraction method for citrus brown blotch germ crude toxin and toxin production condition

InactiveCN104388319AOptimize production conditionsEconomic extraction methodFungiMicroorganism based processesPathogenic bacteriaOrganism
The invention relates to the field of biotechnology, particularly to an extraction method for citrus brown blotch germ crude toxin and a toxin production condition. According to the invention, the preservation number of citrus brown blotch germ is CCTCC M2014484, the method is used for extracting crude toxin, and the obtained citrus brown blotch germ crude toxin is high in content and purity and big in output, short in period and easy to realize large-scale preparation, and besides, the toxin replacing the pathogenic bacteria as a studying object avoids mutual interference and cross contamination among organisms, and the like. According to the invention, a simple, convenient, economic and efficient extraction method for citrus brown blotch germ crude toxin is established, and an excellent foundation is laid for the study of pathogenesis of germ and toxin thereof.
Owner:CITRUS RES INST OF CHINESE ACAD OF AGRI SCI

Citrus bergamot essential oil efficient rapid extraction technology

The invention relates to a citrus bergamot essential oil efficient rapid extraction technology. The technology utilizes crushing, ultrasonic treatment and later extraction processes to prepare high-yield citrus bergamot essential oil.
Owner:杭州析草生物科技有限公司

Extraction method of fish skin active polypeptide

The invention provides an extraction method of fish skin active polypeptide and belongs to the field of polypeptide extraction. The extraction method comprises the following steps: carrying out degreasing treatment, removing hybrid protein, extracting polypeptide and purifying anti-oxidization collagen polypeptide, wherein the method for removing the hybrid protein comprises the following steps: putting fish skin subjected to the degreasing treatment into 0.05 to 0.2 M NaOH and continuously stirring at a low speed; carrying out impurity removal at 5 to 20 DEG C for 12 to 24 h; washing with water until the fish skin is neutral; putting the fish skin into 2 to 3.5 percent NaCl and carrying out salt precipitation for 12 to 24 h; dialyzing with distilled water; taking sediment and freezing anddrying to obtain fish skin collagen powder, wherein 1,2-diphenyl ethylenediamine and semicarbazide are dissolved in the NaOH solution. The fish skin active polypeptide with high purity, low molecularweight and strong anti-oxidization capability is obtained through extraction; the extraction method is simple, environmentally friendly and efficient; the extracted fish skin active polypeptide has low molecular weight, is easy to absorb and has relatively strong anti-oxidization activity, so that the fish skin active polypeptide can be applied to preparation of an anti-oxidization substance in cosmetics, foods and medicines.
Owner:金华市艾力生物科技有限公司

Method for extracting organic oil pollutants in soil

The invention relates to a method for extracting organic oil pollutants in soil. Specific steps of the method includes that soil polluted by the organic oil pollutants is soaked in organic solvent, is ultrasonically extracted at the temperature of 40 DEG C for 20 minutes, then keeps static and is filtered, extracting agent is removed from obtained filtrate, so that extractives are obtained, and frequency of used ultrasonic waves is 40kHz during ultrasonic extraction. The highest extraction efficiency for the pollutants in soil samples can achieve 99%. The method for extracting organic oil pollutants in soil is a new way for ultrasonically extracting the organic pollutants in polluted soil, has the advantages of simplicity, convenience, capability of saving time, high efficiency, low pollution and energy consumption, and the like, and is applicable to be popularized and used as pretreatment technology for polluted soil and sediment samples.
Owner:SHANGHAI UNIV

Water extraction and foam separation method and foam separation device for tea saponin in camellia olefera cake

The invention relates to a water extraction and foam separation method of tea saponin in a camellia olefera cake. The water extraction and foam separation method comprises the steps of water extraction, foam separation, repeated purification and the like of the tea saponin. The invention also relates to a foam separation device; the foam separation device comprises a raw material treatment device,a foam treatment device and a foam reflux device. According to the water extraction and foam separation method and the foam separation device disclosed by the invention, the problems that the extraction rate of the tea saponin is not high and the purity of purification is not high can be well solved, and a green environment-friendly, simple and practical extraction and purification technology ofthe tea saponin is explored.
Owner:CHONGQING UNIV OF TECH

Low-air-permeable coal bed static blasting anti-reflection gas extraction method

The invention discloses a low-air-permeable coal bed static blasting anti-reflection gas extraction method. Multiple roof bedding drill holes are constructed along the coal bed top plate, the drill holes are filled with silent breakers which are subjected to a hydration reaction to generate radial compressive stress and tensile stress to hole walls, when the tensile stress exceeds tensile strengthof coal bed roof rock, the coal bed roof starts to generate cracks, that is to say, a gas migration and output passage is built in the coal bed roof rock, the roof bedding drill holes are subjected to repairing, hole sealing and networking extraction, and coal bed gas is extracted indirectly. Meanwhile, a group of coal bed bedding drill holes are constructed in a coal bed, and migration of the coal bed gas to the roof bedding drill holes is accelerated. After it is determined that extraction reaches the standard, progressive cover type tunneling is conducted, and finally rapid tunneling of alow-air-permeable coal bed coal rod is achieved. Accordingly, by conducting static blasting fracture on the roof bedding drill holes, the gas migration and output passage is built in the coal bed roofrock, and the extraction amount and extraction rate of the low-air-permeable coal bed gas are increased.
Owner:HENAN POLYTECHNIC UNIV

Method for extracting and purifying alkaloid in plants

The invention belongs to the technical field of plant extraction, and particularly relates to a method for extracting and purifying alkaloid in plants and an application. The plant alkaloid is obtained through combination of flash extraction with micro-controlled extraction, and extremely short extraction time and a sufficient and complete distilling manner are used, so that the method disclosed by the invention concurrently has the characteristics of being quick and efficient; the extraction rate and the distilling rate of alkaloid are high, the content of obtained active components is high,the content of impurities is low, the operation time is short, the usage quantity of a solvent is low, and a convenient and efficient obtaining way is provided for extraction and purification of effective components in medical and edible plants.
Owner:GUIZHOU INST OF TECH

Efficient extraction method of inonotus obliquus total triterpenes and preparation method of fine powder of inonotus obliquus total triterpenes

The invention relates to an efficient extraction method of inonotus obliquus total triterpenes and a preparation method of fine powder of the inonotus obliquus total triterpenes. The method comprises the following steps: crushing an inonotus obliquus raw material to obtain inonotus obliquus coarse powder; grinding the inonotus obliquus coarse powder in a liquid-nitrogen low-temperature environment by using a ball mill to obtain inonotus obliquus micropowder; preparing an inonotus obliquus suspension from the inonotus obliquus micropowder; carrying out shearing ultrafine crushing treatment on the inonotus obliquus suspension to obtain an inonotus obliquus ultrafine powder suspension; centrifuging the inonotus obliquus ultrafine powder suspension to obtain a precipitate, and drying the precipitate to obtain inonotus obliquus ultrafine powder; placing the inonotus obliquus ultrafine powder in a steam explosion test device to obtain ultrafine wall-broken powder; adding ethanol into the ultrafine wall-broken powder, and carrying out vacuum combined ultrasonic microwave-assisted extraction to obtain an inonotus obliquus total-triterpene crude extract; and carrying out vacuum concentration to obtain an inonotus obliquus total-triterpene concentrated lixiviate. According to the method, the ethanol dosage and the extraction time are reduced under the same extraction rate, the purity of the obtained triterpenes is high, and the biological activity of the inonotus obliquus total triterpenes is high.
Owner:HEILONGJIANG BAYI AGRICULTURAL UNIVERSITY

Preparation method and use of fish bait for resisting marine pathogenic vibrios

The invention relates to a preparation method of a fish bait for resisting marine pathogenic vibrios. The preparation method comprises the following steps: heating and steaming glutinous rice, adding ethanol with a mass concentration of 30%-70% while the glutinous rice is hot, and controlling a mass volume ratio of the glutinous rice to the ethanol to be (1:0.01)-(1:0.05) g / mL; uniformly mixing and cooling, sequentially adding rape seed cake powder and a fructus corni extracting solution in proportion, and uniformly stirring, chopping and drying to obtain the fish bait; wherein a mass ratio of the glutinous rice to the rape seed cake powder is 1:1 to 1:10, and a mass volume ratio of the glutinous rice to the fructus corni extracting solution is 1:0.1 to 1:10 g / mL. The fructus corni is taken as a raw material, a technology for extracting a fructus corni phenolic acid compound is optimized by adopting a Box-Behnken response surface method, the yield of the fructus corni phenolic acid compound is improved, and an activity of the extract in resisting marine pathogenic bacteria is improved. The prepared fish bait is environmentally friendly, safe, reliable and capable of effectively resisting marine pathogenic bacteria, and has high industrialization value.
Owner:JIANGSU OCEAN UNIV

Method for extracting blue pigment from purple cabbage

The invention discloses a method for extracting a blue pigment from purple cabbage. The method comprises steps as follows: digestion, microwave extraction and purification; purple cabbage pulp is digested through a digestion liquid and then centrifuged, microwave irradiation extraction is performed after a protectant is added to the liquid supernatant, and an ethanol solution of pigment colloid is purified through a silica gel chromatographic column. The method has the benefits as follows: the extraction method is simple and easy to control, consumes short time and is time-saving and labor-saving and suitable for large-scale operation, the process parameters such as temperature, pH values, solid-to-liquid ratio, extraction time, microwave power and the like in an extraction process are effectively controlled, the extraction process is optimized, and the influence of extraction conditions on the blue pigment is reduced to the minimum; a few metal ions in the system can change the color of the pigment and even can change the structure of the pigment, the protectant is added and can form a complex together with the metal ions in the system, and accordingly, the influence of the metal ions on the pigment is avoided. The method for extracting the blue pigment from purple cabbage is safe and efficient.
Owner:浦江县泰如食品科技有限公司

Method for extracting anthocyanin from perilla leaves

The invention belongs to the technical field of extraction methods, and particularly relates to a method for extracting anthocyanin from perilla leaves. The method comprises the following steps: (1) refrigerating the perilla leaves at 0-5 DEG C for 1-2 h; (2) drying the refrigerated perilla leaves for 10-15 min by using hot air at 90-110 DEG C; (3) soaking the perilla leaves dried by the hot air in an aqueous solution at 6-14 DEG C for 10-15 min to obtain an aqueous solution containing the perilla leaves; (4) firstly adding polyethylene glycol into the aqueous solution obtained in the step (3), heating to 30-35 DEG C, then adding a sucrose fatty acid ester / citric acid mixture, and stirring at a high speed for 20-30 min to obtain an extracting solution; and (5) centrifugally separating the extracting solution, taking a supernatant, adding a gel solution into the supernatant, uniformly stirring, and drying at 90-100 DEG C to form a solid. The extraction method is efficient, low in cost, free of toxic reagents, low in energy consumption and easy and convenient to operate.
Owner:贵州侗乡生态农业科技发展有限公司

Method for preparing eugenol from clove oil plant

The invention relates to a method for preparing eugenol from a clove oil plant, specifically to a method for preparing eugenol from clove oil extracted from a syringe plant, and belongs to the biochemical field. The invention relates to a simple, practical and efficient extraction method. Through HPLC detection, the content of the eugenol product extracted by the method reaches more than 98%.
Owner:王喜军 +1

Fungus DNA (Deoxyribose Nucleic Acid) extracting method suitable for PCR (Polymerase Chain Reaction) amplification

The invention relates to a fungus DNA (Deoxyribose Nucleic Acid) extracting method suitable for PCR (Polymerase Chain Reaction) amplification. The method comprises the following steps of: performing liquid culturing on thalli, centrifuging and collecting mycelia; adding a TRIS (tris(hydroxymethyl)aminomethane) saturated phenol extracting solution into grinding equipment for grinding into paste; transferring the paste into a centrifuge tube, adding a chloroform-isopentyl alcohol extracting solution, mixing uniformly, extracting proteins, centrifuging and taking a supernatant; adding absolute ethyl alcohol into the supernatant for precipitating, centrifuging and collecting a precipitate; and washing the precipitate with ethanol, drying and dissolving to obtain a fungus genome DNA. According to the method, the use of liquid ammonia is avoided, the use of reagents is reduced, and a large quantity of samples can be extracted within a short period of time; and the method has the characteristics of easiness, convenience, high efficiency, rapidness and low price. The concentration and purity of the DNA extracted with the method are ideal, and conventional molecular biology researches such as subsequent PCR amplification and the like can be met.
Owner:SHANDONG HOMEY AQUATIC DEV +1

Compound enzyme preparation for oil extraction and oil extraction method

The invention discloses a compound enzyme preparation for oil extraction. The compound enzyme preparation comprises 1 to 3 parts of neutral protease, 1 to 3 parts of alkaline protease, 0.1 to 0.3 partof flavor enzyme, 0.1 to 0.3 part of collagenase and 1 to 3 parts of a carrier in parts by weight. Furthermore, the invention discloses a technological method of the compound enzyme preparation for the oil extraction. The method comprises the following steps: controlling pH to be 7.5 to 8.5, the enzymolysis temperature to be 52 to 65 DEG C, the ratio of materials to liquid to be (2:1) to (4:1), the enzymolysis time to be 2 to 4 hours, and the additive amount to be 0 .1% to 0.5%, thereby being capable of achieving optimum extraction process conditions. The invention has the following technicaleffects: 1) using an enzymolysis method to achieve the purpose of large-scale extraction of oil in slaughtered scraps; 2) using the compound enzyme preparation to extract the oil, thereby being capable of improving the oil extraction rate and oil extraction quality of the slaughtered scraps; 3) precipitating and spray-drying extracted enzymatic hydrolysate and then preparing into high-protein feed, thereby realizing resource utilization and further improving culture benefits.
Owner:龚俊勇 +1

Preparation of eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel

The invention provides a method for preparing an eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel. The optimal conditions for extracting the collagen of the sea eel swimming bladder by the acid method through a single factor test and response surface optimization are as follows: the acetic acid concentration is 0.42 mol / L, the acidolysis time is 21h, and the liquid-material ratio is 58ml / g. The acid-soluble collagen separated from the sea eel swimming bladder can be used as a proper drug carrier system, is a good medicinal biological material, has a unique porous fiber structure, can be used for absorbing a large amount of water and supporting the skin surface, and has important effects on moisturizing and wrinkle resistance; the collagen can be used as a wound excipient or a drug carrier, has a healing promoting effect on a mouse skin wound, and has huge application potential in biomedical engineering and medicine.
Owner:西安华企众信科技发展有限公司

Triple information extraction method and device, electronic equipment and storage medium

The invention relates to the technical field of artificial intelligence, and particularly discloses a triple information extraction method and device, electronic equipment and a storage medium, and the method comprises: performing semantic segmentation on a to-be-extracted text to obtain n words, wherein n is an integer greater than or equal to 1; according to the n words, performing semantic information extraction on the to-be-extracted text to obtain a semantic vector of the to-be-extracted text; determining a main entity word in the n words according to the semantic vector; according to the main entity word and the semantic vector, determining guest entity words corresponding to the main entity word in remaining words, wherein the remaining words are all words except the main entity word in the n words; determining a relation word between the main entity word and the guest entity word according to the main entity word, the guest entity word, the semantic vector and a preset relation database; and taking the main entity words, the guest entity words and the relational words as triple information of the to-be-extracted text.
Owner:PING AN TECH (SHENZHEN) CO LTD
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