Extraction method of fish skin active polypeptide

Abstract

The invention provides an extraction method of fish skin active polypeptide and belongs to the field of polypeptide extraction. The extraction method comprises the following steps: carrying out degreasing treatment, removing hybrid protein, extracting polypeptide and purifying anti-oxidization collagen polypeptide, wherein the method for removing the hybrid protein comprises the following steps: putting fish skin subjected to the degreasing treatment into 0.05 to 0.2 M NaOH and continuously stirring at a low speed; carrying out impurity removal at 5 to 20 DEG C for 12 to 24 h; washing with water until the fish skin is neutral; putting the fish skin into 2 to 3.5 percent NaCl and carrying out salt precipitation for 12 to 24 h; dialyzing with distilled water; taking sediment and freezing anddrying to obtain fish skin collagen powder, wherein 1,2-diphenyl ethylenediamine and semicarbazide are dissolved in the NaOH solution. The fish skin active polypeptide with high purity, low molecularweight and strong anti-oxidization capability is obtained through extraction; the extraction method is simple, environmentally friendly and efficient; the extracted fish skin active polypeptide has low molecular weight, is easy to absorb and has relatively strong anti-oxidization activity, so that the fish skin active polypeptide can be applied to preparation of an anti-oxidization substance in cosmetics, foods and medicines.

Description

technical field [0001] The invention belongs to the field of polypeptide extraction, and in particular relates to a method for extracting fish skin active polypeptides. Background technique [0002] my country has a vast territory, vast waters, and abundant aquatic resources. In recent years, with the improvement of living standards, the diversity and practicability of people's demand for aquatic resources have gradually increased, thus promoting the rapid development of my country's aquatic product processing industry. However, with the rapid development of the aquatic product processing industry, a large amount of fish leftovers, such as fish skin, fish scales, viscera, fish heads, etc., will be produced. Most of the feed is discarded, which not only causes a great waste of resources, but also causes environmental pollution. Therefore, how to efficiently utilize the nutritionally active substances in fish scraps has attracted great attention from scientific researchers at...

AI Technical Summary

Problems solved by technology

[0005] The prior art, such as the Chinese invention patent with the authorized announcement number CN 108531534A, discloses the preparation method of small molecule fish skin polypeptide: firstly, the fish skin is degreased and protein-removed, then swelled with soaking agent, and compound enzyme is added for enzymolysis. Control the temperature, time and pH during the enzymolysis process. After the enzymolysis is over, inactivate the enzyme in a boiling water bath. The supernatant obtained after centrifugation is the fish skin polypeptide hydrolyzate. Purify the fish skin polypeptide hydrolyzate by ultrafiltration, freeze at low temperature, and vacuum dry to obtain the finished product of small molecule fish skin polypeptide; the molecular weight, composition and activity of the small molecule fish skin polypeptide obtained in this invention are unknown, which is not conducive to its further application in cosmetics , health food, medicine and other fields