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Preparation of eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel

A technology of swim bladder collagen and sodium alginate, which is applied to the preparation method of peptides, animal/human protein, medical preparations of non-active ingredients, etc., can solve the problems of polluting the environment and wasting resources, and achieves huge application potential and simple method , easy-to-implement effects

Active Publication Date: 2021-11-16
西安华企众信科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, besides being eaten in a small amount, a large amount of swim bladders are discarded as waste, which not only wastes resources, but also pollutes the environment.

Method used

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  • Preparation of eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel
  • Preparation of eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel
  • Preparation of eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Determination of the Best Extraction Process for Eel Swim Bladder Collagen

[0046]1) Pretreatment: Clean the fresh eel swim bladder, remove the non-swim bladder tissue covered on the swim bladder, blood vessels, fat and surface membrane, clean it with deionized water, cut it into 0.5×0.5cm small pieces, and use 0.1mol / L Soak and stir in sodium hydroxide (1:10w / v) at about 20°C for 48 hours to remove impurities in the swim bladder, replace the sodium hydroxide solution every 6 hours, and then wash the swim bladder with distilled water until the swim bladder appears neutral. Then put the swim bladder into 10% n-butanol solution (1:10w / v), soak and stir at about 20°C for 48 hours to remove the lipid in the swim bladder, and replace the n-butanol solution every 6h 1 time, and then use distilled water to clean the n-butanol in the swim bladder; store the cleaned eel swim bladder at -20°C for later use.

[0047] 2) Single factor test

[0048] According to the ins...

Embodiment 2

[0065] The property analysis of the ASC that embodiment 2 optimal process prepares

[0066] To verify the predicted yield under optimal extraction conditions, three replicate experiments were performed. The average extraction rate of eel swim bladder collagen is 65.32%, which is very consistent with the predicted value. At the same time, the properties of the extracted eel swim bladder ASC were determined:

[0067] 1) Ultraviolet full-wavelength scanning (UV) analysis of eel swim bladder ASC

[0068] Dissolve ASC in 0.5mol / L acetic acid solution to prepare a 1mg / ml ASC solution, and use 0.5mol / L acetic acid solution as a blank control, and use a UV spectrophotometer to scan it at an ultraviolet wavelength in the range of 190-400nm. Such as Figure 4 It can be seen that there is a sharp and strong absorption peak at a wavelength of 225nm, indicating that hydroxyproline and proline may exist in the swim bladder collagen solution, and the absorption peak at 225 nm indicates th...

Embodiment 3

[0078] Example 3 Preparation of eel swim bladder collagen-chitosan-composite sodium alginate hydrogel

[0079] 1) Pretreatment: Clean the fresh eel swim bladder, remove the non-swim bladder tissue covered on the swim bladder, blood vessels, fat and surface membrane, clean it with deionized water, cut it into 0.5×0.5cm small pieces, and use 0.1mol / L Soak and stir in sodium hydroxide (1:10w / v) at about 20°C for 48 hours to remove impurities in the swim bladder, replace the sodium hydroxide solution every 6 hours, and then wash the swim bladder with distilled water until the swim bladder appears neutral. sex. Then put the swim bladder into 10% n-butanol solution (1:10w / v), soak and stir at 20°C for 48 hours to remove the lipid in the swim bladder, and replace the n-butanol solution once every 6 hours , and then clean the n-butanol in the swim bladder with distilled water. Store the cleaned eel swim bladder at -20°C for later use.

[0080] 2) According to Example 1, the best pr...

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Abstract

The invention provides a method for preparing an eel swimming bladder collagen-chitosan-composite sodium alginate hydrogel. The optimal conditions for extracting the collagen of the sea eel swimming bladder by the acid method through a single factor test and response surface optimization are as follows: the acetic acid concentration is 0.42 mol / L, the acidolysis time is 21h, and the liquid-material ratio is 58ml / g. The acid-soluble collagen separated from the sea eel swimming bladder can be used as a proper drug carrier system, is a good medicinal biological material, has a unique porous fiber structure, can be used for absorbing a large amount of water and supporting the skin surface, and has important effects on moisturizing and wrinkle resistance; the collagen can be used as a wound excipient or a drug carrier, has a healing promoting effect on a mouse skin wound, and has huge application potential in biomedical engineering and medicine.

Description

technical field [0001] The invention belongs to the field of biological extraction, in particular to the preparation of eel swim bladder collagen-chitosan-composite sodium alginate hydrogel Background technique [0002] The skin is the largest sensory organ in the human body, and it is prone to accidental damage during work. During wound healing, a complex combination of epithelial tissue regeneration, granulation tissue formation and hyperplasia, and scar connective tissue formation is required. Collagen deposition and blood vessel formation are key factors. Key to wound healing. Traditional wound dressings mainly use natural fiber materials such as absorbent cotton gauze and gauze strips, which can dry the wound and provide physical isolation. However, it is easy to tear the wound when changing the dressing, causing re-bleeding, which is not conducive to healing. [0003] The eel industry in my country has developed tremendously. The eel meat is delicious and has high nut...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00A61K9/06A61K47/42A61K47/36C07K14/78C07K1/12C07K1/34C07K1/14
CPCA61L26/0033A61L26/0023A61L26/008A61L26/0085A61K9/06A61K47/42A61K47/36C07K14/78C08L89/00C08L5/08C08L5/04Y02A50/30
Inventor 莫奕雯杨最素郑佳文田笑笑李航婷金明月田静李诺营
Owner 西安华企众信科技发展有限公司
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