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Method for extracting anthocyanin from perilla leaves

A technology of perilla leaves and anthocyanins, which is applied in the field of extracting anthocyanins, can solve the problems of reducing stability, being susceptible to the external environment, neglecting, etc.

Pending Publication Date: 2021-09-10
贵州侗乡生态农业科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The current extraction technology has largely ignored the stability of anthocyanins, but because the basic structure of anthocyanins is a diphenylbenzopyran cation, which lacks an electron in the structure, anthocyanins have strong antioxidant properties, making anthocyanins easy to extract It is affected by the external environment, which increases its instability, and the pH value, temperature, oxygen, ascorbic acid, flavonoids, proteins and metal ions will all reduce its stability

Method used

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  • Method for extracting anthocyanin from perilla leaves
  • Method for extracting anthocyanin from perilla leaves
  • Method for extracting anthocyanin from perilla leaves

Examples

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Effect test

Embodiment 1

[0032] A method for extracting anthocyanins from perilla leaves, comprising the steps of:

[0033] 1) Take perilla leaves and refrigerate at 5°C for 2 hours;

[0034] 2) Dry the refrigerated perilla leaves with hot air at 110°C for 15 minutes;

[0035] 3) According to the mass ratio of material to liquid ratio of 1:10, the hot-air dried perilla leaves were soaked in an aqueous solution at 6-14°C for 15 minutes to obtain an aqueous solution containing perilla leaves;

[0036] 4) Add polyethylene glycol to the aqueous solution obtained in step 3), heat up to 35°C, then add the sucrose fatty acid ester / citric acid mixture, and stir at high speed for 30 minutes to obtain the extract;

[0037] 5) After the extract is centrifuged, take the supernatant, add the gel solution to the supernatant, stir evenly, and dry at 100°C to form a solid;

[0038] Described polyethylene glycol, its quality is 70% of aqueous solution;

[0039] The molecular weight of the polyethylene glycol is 600...

Embodiment 2

[0047] A method for extracting anthocyanins from perilla leaves, comprising the steps of:

[0048] 1) Take perilla leaves and refrigerate at 0°C for 1 hour;

[0049] 2) Dry the refrigerated perilla leaves with hot air at 90°C for 10 minutes;

[0050] 3) According to the mass ratio of material to liquid ratio of 1:8, the hot-air dried perilla leaves were soaked in an aqueous solution at 6°C for 10 minutes to obtain an aqueous solution containing perilla leaves;

[0051] 4) Add polyethylene glycol to the aqueous solution obtained in step 3), heat up to 35°C, then add the sucrose fatty acid ester / citric acid mixture, and stir at high speed for 20 minutes to obtain the extract;

[0052] 5) After the extract is centrifuged, take the supernatant, add the gel solution to the supernatant, stir evenly, and dry at 90°C to form a solid;

[0053] Described polyethylene glycol, its quality is 60% of aqueous solution;

[0054] The molecular weight of the polyethylene glycol is 500;

[0...

Embodiment 3

[0062] A method for extracting anthocyanins from perilla leaves, comprising the steps of:

[0063] 1) Take perilla leaves and refrigerate at 2°C for 1.5h;

[0064] 2) Dry the refrigerated perilla leaves with hot air at 100°C for 12 minutes;

[0065] 3) According to the mass ratio of material to liquid ratio of 1:9, the hot-air dried perilla leaves were soaked in an aqueous solution at 10°C for 12 minutes to obtain an aqueous solution containing perilla leaves;

[0066] 4) Add polyethylene glycol to the aqueous solution obtained in step 3), heat up to 32°C, then add the sucrose fatty acid ester / citric acid mixture, and stir at high speed for 25 minutes to obtain the extract;

[0067] 5) After the extract is centrifuged, take the supernatant, add the gel solution to the supernatant, stir evenly, and dry at 95°C to form a solid;

[0068] Described polyethylene glycol, its quality is 65% of aqueous solution;

[0069] The molecular weight of the polyethylene glycol is 550;

[0...

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Abstract

The invention belongs to the technical field of extraction methods, and particularly relates to a method for extracting anthocyanin from perilla leaves. The method comprises the following steps: (1) refrigerating the perilla leaves at 0-5 DEG C for 1-2 h; (2) drying the refrigerated perilla leaves for 10-15 min by using hot air at 90-110 DEG C; (3) soaking the perilla leaves dried by the hot air in an aqueous solution at 6-14 DEG C for 10-15 min to obtain an aqueous solution containing the perilla leaves; (4) firstly adding polyethylene glycol into the aqueous solution obtained in the step (3), heating to 30-35 DEG C, then adding a sucrose fatty acid ester / citric acid mixture, and stirring at a high speed for 20-30 min to obtain an extracting solution; and (5) centrifugally separating the extracting solution, taking a supernatant, adding a gel solution into the supernatant, uniformly stirring, and drying at 90-100 DEG C to form a solid. The extraction method is efficient, low in cost, free of toxic reagents, low in energy consumption and easy and convenient to operate.

Description

technical field [0001] The invention belongs to the technical field of extraction methods, in particular to a method for extracting anthocyanins from perilla leaves. Background technique [0002] Perilla is an annual herb of Lamiaceae. Perilla leaves are rich in anthocyanins, including 9 kinds of anthocyanins and cis-isomers, among which the highest content is malonyl perilla and perilla. Anthocyanins exhibit various physiological activities in terms of anti-oxidation, antibacterial, anti-inflammation, anti-cancer and anti-hyperglycemia. [0003] Common anthocyanin extraction methods mainly include solvent method, ultrasonic-assisted extraction method, microwave-assisted extraction method, ultra-high pressure assisted extraction method, etc., but anthocyanins are easily degraded at high temperature. [0004] The patent application number CN201610196610.2 discloses a method for extracting ursolic acid and anthocyanins from perilla leaves, and adopts the method of killing gre...

Claims

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Application Information

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IPC IPC(8): C07H17/065C07H1/08
CPCC07H17/065C07H1/08
Inventor 刘荣贵许志祥
Owner 贵州侗乡生态农业科技发展有限公司
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