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Highly effective extraction method for magnolol and honokiol crude extract

A technology of honokiol and an extraction method, applied in the field of extraction of active ingredients of medicinal plants, can solve the problems of unsafe, expensive extraction equipment, oxidation and the like

Inactive Publication Date: 2008-02-20
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Alkaline extraction is easy to oxidize phenolic substances such as magnolol; supercritical CO 2 The extraction equipment is expensive and the cost is too high; and magnolol is not easily soluble in water, so only ethanol extraction is more suitable for the extraction of magnolol and honokiol
Since magnolol and honokiol are used in food and medicine, the traditional ethanol extraction method uses high-concentration industrial alcohol with a large solid-to-liquid ratio and a long extraction time. Therefore, this method is unsystematic and unsafe. , and the cost is too high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Weigh 500g of magnolia bark powder, extract it with 5000mL 50% edible ethanol at 80°C for 3 hours, then filter it while it is hot, cool the filtrate quickly, and extract the residue with 5000mL 50% edible ethanol at 80°C for 3 hours and then extract it while it is hot Filtrate, rapidly cool the filtrate, pass the combined filtrate through 200-mesh nylon cloth, and use a high-speed centrifuge to centrifuge and clarify, take the clarified liquid and concentrate it with a vacuum rotary evaporator at 42 ° C, recover the edible ethanol solvent and vacuum freeze-dry to obtain magnolol With the crude extract 69.235g of honokiol, the content of magnolol and honokiol in the extract recorded by high performance liquid chromatography is 11.82% and 7.88%, respectively, and the content of magnolol and honokiol The extraction rates measured by the oven method were 73.52% and 70.42%, respectively.

Embodiment 2

[0017] Weigh 500g of magnolia bark powder, extract it with 5000mL of 60% edible ethanol at 85°C for 2.5 hours, then filter it while it is hot, cool the filtrate quickly, and extract the residue with 3000mL of 60% edible ethanol at 85°C for 2.5 hours, then extract it while it is hot Filtrate, quickly cool the filtrate, pass the combined filtrate through 200-mesh nylon cloth, and use a high-speed centrifuge to centrifuge and clarify, take the clarified liquid and concentrate it at 40°C with a vacuum rotary evaporator, recover the edible ethanol solvent and vacuum freeze-dry to obtain magnolol With the crude extract 77.34g of honokiol, the content of magnolol and honokiol in the extract recorded by high performance liquid chromatography is 12.49% and 8.95%, respectively, and the content of magnolol and honokiol The extraction rates measured by the oven method were 86.78% and 89.34%, respectively.

Embodiment 3

[0019] Weigh 500g of magnolia bark powder, extract it with 5000mL 70% edible ethanol at 90°C for 2 hours, then filter it while it is hot, cool the filtrate quickly, and extract the residue with 1000mL 70% edible ethanol at 90°C for 2 hours, then extract it while it is hot Filtrate, quickly cool the filtrate, pass the combined filtrate through 200-mesh nylon cloth, and use a high-speed centrifuge to centrifuge and clarify, take the clarified liquid and concentrate it at 45°C with a vacuum rotary evaporator, recover the edible ethanol solvent and vacuum freeze-dry to obtain magnolol With the crude extract 73.50g of honokiol, the content of magnolol and honokiol in the extract recorded by high performance liquid chromatography is 10.89% and 7.585%, respectively, and the content of magnolol and honokiol The extraction rates measured by the oven method were 71.91% and 71.96%, respectively.

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Abstract

The invention discloses a method for extracting magnolol and honokiol crude extract with high efficiency, the method is that: the magnolol is dried up and crushed and sieved into 30 mesh, the magnolol is added to edible alcohol twice at the weight proportion of one grams of magnolol powder to 12 to 20ml edible alcohol, the extract temperature is 80 to 90 DEGC, the extract time is 2.0 to 3.0 hours each time, the concentration of edible alcohol is 50 to 70 percent, the two extraction crude filter liquors are combined and sieved into 200-mesh nylon fabric, a high speed centrifuge is used for centrifuging and clarifying and a clear liquid is taken out; the clear liquid at the temperature of 40 to 45 DEG C is carried out vacuum concentration by a rotary evaporator, the edible alcohol solvent is recovered and is frozen and dried in vacuum so as to obtain the crude extract, the extract rate of magnolol and honokiol is obviously higher than that of the prior art, the amounted manufacturing cost is relatively low. The method is harmless to the human body as well as to the environment by adopting edible alcohol for extraction; therefore, the method is particularly safe.

Description

Technical field: [0001] The invention relates to a technology for extracting effective components of medicinal plants, in particular to a high-efficiency extraction method for magnolol and honokiol crude extract. Background technique: [0002] Magnolia officinalis Rehd.et Wils. and M.biloba (Rehd.et Wils.) Cheng, Magnolia officinalis Rehd.et Wils., a deciduous tree of Magnoliaceae, are used as medicine with dry bark, branch bark and root bark. The active ingredients in magnolia bark mainly include magnolol (magnolol), honokiol (honokiol), isomagnolol, trihydroxy magnolol, destrihydroxy magnolol, wherein magnolol, honokiol Mainly, accounting for about 3% to 5% of the original medicinal materials. [0003] In order to give full play to the pharmacological efficacy of magnolia bark, there are currently many studies on the extraction of magnolol and honokiol, mainly including alkali extraction and acid precipitation method, water extraction method, ethanol extraction method, al...

Claims

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Application Information

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IPC IPC(8): C07C39/21C07C37/68A61K36/57
Inventor 肖文军傅冬和李国莉
Owner HUNAN AGRICULTURAL UNIV
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