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Extraction method of selenoprotein in cardamine violifolia, selenoprotein obtained by extraction method and application of selenoprotein

A technology of crushed cinnamon leaves and extraction methods, which can be used in applications, protein food processing, plant protein processing, etc., can solve the problems of multiple impurities, high production costs, and long time consumption, and achieve simple extraction methods, low cost, and high efficiency. high effect

Active Publication Date: 2021-03-19
ENSHI QINGJIANG BIO ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The existing technology for extracting selenoproteins in Corydalis chinensis generally adopts hot acid solution extraction or alkaline solution extraction, which takes a long time in the extraction process, has high production costs, produces more impurities, and the extracted product contains Contains inorganic selenium

Method used

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  • Extraction method of selenoprotein in cardamine violifolia, selenoprotein obtained by extraction method and application of selenoprotein
  • Extraction method of selenoprotein in cardamine violifolia, selenoprotein obtained by extraction method and application of selenoprotein
  • Extraction method of selenoprotein in cardamine violifolia, selenoprotein obtained by extraction method and application of selenoprotein

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0085] (1) Get 3kg Viola Leaf Broken Rice Chestnut Powder (total selenium content: 500mg / kg), add 75L of purified water, obtain the mixed solution and adjust the pH to 5.0 with food-grade acetic acid, add cellulase, hemicellulase and wood Glycanase, stirred for 2 hours, and kept the temperature in the enzymolysis process at 50°C in a constant temperature water bath, wherein the dosages of cellulase, hemicellulase and xylanase were 90g, 30g and 3g respectively; The mixture after the solution is filtered with a filter plate, and the filtrate obtained continues to be centrifuged using a disc centrifuge to collect the centrifugate; in this centrifugal process, the feed rate is 0.8t / h, and the disc centrifuge rotating speed is 6800r / min. The slag discharge time is 3s, the slag discharge cycle is 5min, and the flushing time is 3s.

[0086] (2) Pass the centrifugate obtained in step (1) through a microfiltration membrane, the microfiltration membrane is a hollow fiber membrane with a...

Embodiment 2

[0089] (1) Get 3kg Viola Leaf Broken Rice Chestnut Powder (total selenium content: 500mg / kg), add 60L of purified water, obtain the mixed solution and adjust the pH to 4.0 with food-grade acetic acid, add cellulase, hemicellulase and wood Glycanase, stirred for 1 hour, and kept the temperature at 45°C in a constant temperature water bath, wherein the dosages of cellulase and xylanase were 75g, 45g and 4.5g respectively; then the mixture after enzymolysis Use a filter plate to filter, and the obtained filtrate continues to be centrifuged with a disc centrifuge to collect the centrifugate; during the centrifugation process, the feed rate is 1t / h, the disc centrifuge speed is 7000r / min, and the slag discharge time is 2s. The slagging period is 4min, and the flushing time is 2s.

[0090] (2) Pass the centrifugate obtained in step (1) through a microfiltration membrane, the microfiltration membrane is a hollow fiber membrane with a molecular weight of 400,000Da, the membrane apertu...

Embodiment 3

[0093] (1) Get 3kg Viola leaf broken rice chestnut powder (total selenium content: 500mg / kg), add 90L of pure water, obtain the mixed solution and adjust pH with food-grade acetic acid to be 6.0, add cellulase, hemicellulase and wood Glycanase, stirred for 3 hours, and kept the temperature in the enzymolysis process at 40°C in a constant temperature water bath, wherein the dosages of cellulase and xylanase were 60g, 60g and 6g respectively; then the mixture after enzymolysis was used The filter plate is filtered, and the obtained filtrate continues to be centrifuged with a disc centrifuge to collect the centrifugate; during the centrifugation process, the feed rate is 0.8t / h, the disc centrifuge speed is 6700r / min, and the slag discharge time is 2s. The slagging period is 3min, and the flushing time is 2s.

[0094](2) Pass the centrifugate obtained in step (1) through a microfiltration membrane, the microfiltration membrane is a hollow fiber membrane with a molecular weight of...

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Abstract

The invention provides an extraction method of selenoprotein in cardamine violifolia, the selenoprotein obtained by the extraction method and application of the selenoprotein. The extraction method comprises the following steps of carrying out enzymolysis on the cardamine violifolia by adopting enzymes including cellulase to obtain a cardamine violifolia enzymatic hydrolysate; and concentrating the cardamine violifolia enzymatic hydrolysate, and drying the concentrated cardamine violifolia enzymatic hydrolysate. According to the method disclosed by the invention, the selenoprotein in the cardamine violifolia is extracted through enzymolysis of the enzymes including the cellulase, so that nutritional ingredients in the cardamine violifolia are not damaged, the efficiency of extracting the selenoprotein through enzymolysis is high, the yield of the selenoprotein is relatively high, the protein content in the selenoprotein is high, the content of total selenium is high, and the total selenium is organic selenium; and in addition, the extraction method is simple, efficient, low in cost, environmentally friendly and easy for large-scale industrial production.

Description

technical field [0001] The invention relates to the technical field of biological extraction, and in particular to a method for extracting selenoproteins in broken Viola leaves, selenoproteins obtained by the extraction method and applications thereof. Background technique [0002] In recent years, studies have found that the genus Myrina has a super selenium-accumulating ability. At present, as the public's understanding of the relationship between selenium and health is getting deeper and deeper, the research on selenoproteins in Viola sativa has gradually become a hot spot. The main form of selenium in Violet-leaf catkins is organic selenium. There are a few patents and literature reports on the extraction and preparation of selenoproteins from Viola-leaf catkins. [0003] The existing technology for extracting selenoproteins in Viola leaf broken rice chestnuts generally adopts hot acid solution extraction method or alkaline solution extraction method, which takes a long...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23J1/00A23J3/14A23J3/34A23L33/185
CPCA23J1/006A23J3/14A23J3/346A23L33/185Y02P20/10
Inventor 龙澜杨永康向极钎殷红清问小龙张亮万海英李亚杰田腾飞
Owner ENSHI QINGJIANG BIO ENG
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