Method for extracting blue pigment from purple cabbage
A technology of blue pigment and purple cabbage, applied in chemical instruments and methods, azo dyes, organic dyes, etc., can solve the problems of limited promotion significance, time-consuming and labor-consuming, cumbersome preparation methods, etc., and achieve simple and easy-to-control extraction methods, The effect of short time and optimized extraction process
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Embodiment 1
[0016] A method for extracting blue pigment from purple cabbage, including the following steps:
[0017] 1) Take fresh and non-rotted purple cabbage, rinse with clean water, dry and chop, accurately weigh 20g sample, add 400g distilled water to make a slurry, 30°C constant temperature water bath for 40 minutes, the extract is centrifuged at 4000r / min for 10 minutes, take Supernatant; 2) Place the leached supernatant in a container under dark conditions, adjust the temperature to 55°C, add 0.30mol / L hydrochloric acid to adjust the pH to 2, and add 0.010g of active peptide at the same time. The crude extract is obtained by microwave radiation at 550W power for 3 minutes; when the pH value of the pigment extract is in the range of 2-3, the pigment extract can exist stably, which is the best pH value for extracting pigment; 3) Pigment After the crude extract passes through the rotary evaporator, it is dried in a vacuum oven to obtain the pigment colloid. The pigment colloid is dissol...
Embodiment 2
[0019] A method for extracting blue pigment from purple cabbage includes the following steps:
[0020] 1) Extraction: The fresh purple cabbage is beaten and then extracted with distilled water, and the supernatant is taken after centrifugation; the extraction can remove the impurities such as cellulose in the purple cabbage and prepare for further extraction;
[0021] 2) Microwave extraction: place the leached supernatant in a container under dark conditions, adjust the temperature to 60°C, add 0.35mol / L hydrochloric acid to adjust the pH to 3, and add 0.012 parts of active peptide at the same time. Microwave radiation at 600W power for 5 minutes to obtain a crude extract; metal ions can change the color of the pigment, and even change the structure of the pigment, and the protective agent can form a complex with the metal ions in the system to avoid metal ions Effect on pigment;
[0022] 3) Purification: The crude pigment extract is dried in a vacuum oven to obtain the pigment coll...
Embodiment 3
[0024] A method for extracting blue pigment from purple cabbage, including: extraction, microwave extraction, and purification, specifically including the following steps:
[0025] Extraction: Take fresh and non-rotted purple cabbage, rinse with clean water, dry and chop, accurately weigh 30 samples, add 750 parts of 30wt.% ethanol solution to make the pulp, 32 ℃ constant temperature water bath for 45 minutes, the extract is Centrifuge at 4200r / min for 115 minutes, and take the supernatant; extraction can remove impurities such as cellulose in the purple cabbage to prepare for further extraction;
[0026] Microwave extraction: place the leached supernatant in a container under dark conditions, adjust the temperature to 60°C, add 0.30mol / L hydrochloric acid to adjust the pH to 2.2, and add 0.010 parts of active peptides at 580W The crude extract is obtained by microwave radiation at power for 5 minutes; when the pH value of the pigment extract is in the range of 2-3, the pigment ext...
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