30 results about "Virus Structure" patented technology

The invention discloses a synthesis method of a visual guided tumor combined immunotherapy nano-preparation for Gd:CuS mineralized influenza virus (Flu@Gd:CuS) and discloses a method for successfullysynthesizing the tumor combined therapy nano-preparation by strategy bionic simulation of Flu@Gd:CuS. The method comprises the main steps as follows: 1) preparation of a CuC12.2H2O aqueous solution; 2) preparation of a GdC13.6H2o aqueous solution; 3) synthesis of Flu@Gd:CuS nanoparticles by a one-pot method. Influenza virus structure is complex, the organism immune function of the whole body can be activated, and all-around killing and clearing of tumor can be realized. The Flu@Gd:CuS nanoparticles have significant T1 weighed magnetic resonance/opto-acoustic dual-mode imaging capacity, have higher photo-thermal conversion efficiency when exposed to near infrared laser, can efficiently guide following tumor photo-thermal therapy, have excellent biocompatibility and have no side effects.

A self-service respiratory vaccination instrument for SARS infectious disease belongs to respiratory tract communicable disease treating, defending and spread proof medical apparatus. The self-service respiratory vaccination instrument uses instruments means to create high temperature as in summer, and uses the penetrating power of damp-heat water vapour, or the destructive power to the virus structure from negative oxygen ion and pH regulating agent, or the killing power to the microorganism from plant essence oil to make the respiratory tract virus, pathogenic bacteria such as 'SARS' to be attenuated, killed or inactivated; the microorganism attenuated, killed or inactivated in the respiratory tract and its giblets become specificity vaccine for the microorganism and are disposed in the respiratory tract, the effect that the 'SARS' respiratory tract microorganism vaccine can be inoculated to the respiratory tract with no time difference and no other outer vaccine can be realized, and the object of the cure, defense and diffuse proof for corresponding respiratory tract infectious diseases can be achieved.

The preparation process of reovirus antigen subunit vaccine for grass carp includes the following steps: proliferation of GCRV873, which is the Hunan Shaoyang strain of reovirus, in grass carp kidney cell line; centrifugal purification and concentration of GCRV873 infected cell virus suspension; processing purified virus with surfactant to disintegrate complete virus structure to form viral nucleic acid and capsid protein subunit component; digesting with nuclease to degrade free virus genome dsRNA; dialysis to obtain reovirus protein subunit antigen preparation for grass carp containing no nuclease; and diluting with physiological saline to obtain the said vaccine. The vaccine of the present invention has the advantages of containing complete virus protein antigen component, containing no genetic virus matter RNA, simple preparation process, high product purity, etc.

The invention belongs to the technical field of prevention and treatment on novel coronavirus and particularly discloses a method and equipment for effectively deactivating viruses. The method comprises the steps: S1. carrying out charging treatment on aerosol possibly containing pathogens; S2. applying an external electric field to the charged aerosol, so as to collect the aerosol in the presenceof an electric field force; and S3. carrying out virus killing treatment on the collected aerosol to destroy RNA structures of the viruses in the aerosol, thereby effectively deactivating the viruses. According to the method and the equipment, charged high-voltage line poles charge the aerosol possibly containing the pathogens through a pulsed high-voltage power supply, the external electric field is applied to the charged aerosol to collect the charged aerosol in the presence of the electric field force, meanwhile, the collected aerosol is irradiated with powerful ultraviolet radiation to destroy the RNA structures of the viruses in the aerosol and enable the pathogens in the aerosol to lose reproduction capability or activity, and thus, the viruses are effectively deactivated.

The invention belongs to the field of high-polymer materials and discloses an imitated virus-structured high-polymer vesicle with a target function and preparation and application thereof. The preparation method of the imitated virus-structured high-polymer vesicle with the target function comprises, S1, performing amide reaction on hyaluronic acid containing amino and hydrophobic polymers containing carboxyl to obtain hyaluronic acid grafted amphiphilic block copolymers, wherein the hydrophobic polymers containing carboxyl requires carboxyl activation before reaction are performed, and the hyaluronic acid containing amino are obtained through reaction between diamine and hyaluronic acid; S2, reacting the hyaluronic acid grafted amphiphilic block copolymers with other amphiphilic block copolymers through a solvent exchange method or a hydration method to obtain the imitated virus-structured high-polymer vesicle with the target function. The preparation method of the imitated virus-structured high-polymer vesicle with the target function is simple in operation, flexible in modification, high in production efficiency and good in repeatability; the prepared imitated virus-structured high-polymer vesicle achieves the target function and can be applied to the biomedical fields such as drug carriers.

The invention belongs to the field of high polymer materials and discloses a virus structure-imitated high-polymer vesica as well as a preparation method and application thereof. The virus structure-imitated high-polymer vesica is prepared from different amphipathic block copolymers through self-assembling or is prepared from amphipathic block copolymers and water-soluble polymers through self-assembling. The virus structure-imitated high-polymer vesica comprises a capsid membrane structure and a spike structure on the surface of a membrane, wherein the inner layer and the outer layer of the capsid membrane structure consist of hydrophilic chain segments in the amphipathic block copolymers or the water-soluble polymers, and an intermediate layer between the inner layer and the outer layer consists of hydrophobic chain segments in the amphipathic block copolymers. The high-polymer vesica has a virus-imitated specific structure and is controllable in structure, high in repeatability, capable of carrying drugs, good in biocompatibility and easy to be phagocytized by cells; the operation of the method is simple, and the production efficiency is high; and meanwhile, the high-polymer vesica can be applied to the relevant fields of high-efficiency nano-carriers, controlled release of medicines and cell imaging.

The invention belongs to the field of pharmacy, and relates to application of wogonin in preparing medicine for resisting a herpes simplex virus. An experiment verifies that wogonin has a high activity of resisting the herpes simplex virus in an in-vitro experiment and can inhibit the expression of virus structure protein gD in mRNA transcription and the protein level; an in-vitro toxicity experiment of wogonin finds that the flavone type compound has low toxicity to cells of the human and African green monkeys and can obtain a high treatment index; therefore, wogonin has potential value as novel medicine resisting the herpes simplex virus and can be applied to preparing the medicine for resisting the herpes simplex virus.

The invention provides a DNA virus reference material and a preparation method and application thereof. The DNA virus reference material is recombinant viruses which are obtained by recombining coding genes of nucleocapsid protein in the DNA viruses into baculovirus to be expanded and purified. The DNA virus reference material prepared from baculovirus contains a virus structure and can serve as a parallel contrast in the sample processing and extracting process, and conduct quality control on the operation technology. In addition, the prepared reference material can conduct quality control on agents used in the sample processing process and provide powerful guarantees for effectiveness of experimental results.

The invention discloses an antibacterial and antiviral ferritic stainless steel and a preparation method thereof. According to the invention, multiform alloying silver elements and rare earth elements are distributed in a ferritic structure; the silver elements are naturally activated by the environment to form silver ions with different valence states, and the silver ions can act on bacteria and virus independently or in a combined mode; so that bacteria respiration is inhibited, or a virus structure is damaged; and therefore the bacteria and virus is killed; and the rare earth elements can increase the corrosion resistance of the steel and improve the antibacterial activity of the steel at the same time, and the requirements of kitchens and bathrooms, household appliances, household appliance parts, tableware, other public facilities and the like for stainless steel materials are met advantageously.

The invention discloses an LMP-2 recombinant adeno-associated virus vector and a construction method and an application thereof. The LMP-2 recombinant adeno-associated virus vector is obtained by replacing an adeno-associated virus structure gene in an adeno-associated virus vector with an LMP-2 gene or a mutant gene thereof. A wild type or mutant type LMP-2 gene carried by the LMP-2 recombinant adeno-associated virus vector can be transported in a monocyte-macrophage-dendritic cell line by the LMP-2 recombinant adeno-associated virus vector, and cells carrying specific antigen genes are usedto stimulate effector cells of an immune system. Experiments show that CTL induced by DC and infected by rAAV of the invention can effectively inhibit the growth of malignant tumor cells or kill tumorcells in the body of patients, and therefore, the LMP-2 recombinant adeno-associated virus vector of the invention or products related to the LMP-2 recombinant adeno-associated virus vector can be used for preparing antitumor drugs.

The invention discloses a nano bionic enhanced gram-positive bacterium capturing-separating agent as well as a preparation method and application thereof. The capture-separation agent comprises a Fe3O4 magnetic core, and the exterior of the Fe3O4 magnetic core is coated with tannic acid; the tannic acid is granular, so that the whole separating agent is a separating agent with a structure similar to a virus structure, wherein the particles can capture and separate bacteria in fruit juice. According to the invention, the nano material can specifically recognize gram-positive bacteria and can capture and separate gram-positive bacteria in fruit juice, the capture efficiency of the nano capture separating agent is remarkably improved through a viral structure and tannic acid modification, and high specificity is achieved in gram-positive bacterium adsorption and separation.

The present invention provides recombinant T4 bacteriophage expressing infectious chicken bursal disease virus structure protein VP2. The recombinant T4 bacteriophage has the nucleotide sequence of infectious bursal disease virus VP2 gene and the amino acid residue sequence of VP2 protein. The present invention also relates to the application of the recombinant T4 bacteriophage in the immunity with infectious bursal disease vaccine and the detection of infectious bursal disease virus.

The invention provides recombinant hansenula polymorpha capable of expressing Zika virus E protein under the assistance of a molecular chaperone and a construction method of the recombinant hansenulapolymorpha. The construction method comprises the following steps: firstly, constructing a coexpression vector of recombinant Zika virus structural protein E and molecular chaperone hansenula polymorpha calcium connexin, wherein the sequence of the recombinant Zika virus structural protein E is shown as SEQ.ID.NO.1 and the gene fragment of the hansenula polymorpha calcium connexin is shown as SEQ.ID.NO.2; and secondly, transforming the linearized coexpression vector into a hansenula polymorpha cell through electroporation transformation, performing induction culture, purifying a recombinant hansenula polymorpha fermentation product, and then detecting that the recombinant protein content can reach 12.6 mg/L.

The invention relates to the technical field of graphic processing, in particular to a web-based chilled electron microscope data analysis graphic system and method. The system comprises a visualization module, a segmentation module and a matching module. The visualization module comprises volume rendering, iso-surface and crystal model visualization, and chilled electron microscope data comprisesdensity data and crystal data. According to the invention, the visualization module can display data content; data content can be visually seen; the whole three-dimensional data volume is segmented according to the characteristics of a polyhedron structure, global segmentation of molecules is achieved through a multi-target fast marching method, the segmentation speed is increased, the situationthat virus structure characteristics are rapidly obtained for biologists through manpower is reduced, and memory consumption in an automatic segmentation algorithm is effectively reduced through a newdata structure; the multi-target fast marching method supports simultaneous segmentation of a multi-target region; the algorithm directly provides automatic selection of initial seed points; and a second step is added in the loop to ensure that each point is not segmented by a plurality of regions.

The invention discloses a lentivirus dissolution buffer solution and application thereof. The lentivirus dissolution buffer solution comprises 3-10 mM of Tris, 15-35 mM of proline, 1-5 mM of MgCl2, 5-20% of lactose, 0.005-0.02% of Tween 80 and the balance of water, the percentage is mass percent, and the pH value of the lentivirus dissolution buffer solution is 7-9. The components of the lentivirus dissolution buffer solution are all pharmaceutic adjuvants, the influence on the lentivirus activity can be avoided, and the virus structure can be stabilized and the virus activity can be enhanced by adding proline into the lentivirus dissolution buffer solution; the Tween80 can be used for preventing protein or virus aggregation; and MgCl2 has the effect of maintaining protein or virus activity, through the combined effect of several components in the formula, the activity and recovery rate of lentivirus during purification can be comprehensively improved, and the transduction efficiency of lentivirus and the positive rate of transduction CAR can be improved.