Enveloped ribonucleic acid (RNA) virus nucleic acid detection reference/standard platform and application method thereof

Abstract

The invention particularly relates to an enveloped ribonucleic acid (RNA) virus nucleic acid detection reference / standard platform. The enveloped RNA virus nucleic acid detection reference / standard platform is mainly prepared by using a vector on which an exogenous target sequence is carried, a vector containing an MLV gag-pol component, and a plasmid which comprises or does not comprise a viral membrane protein to cotransfect a reference producing cell strain, is a similar virus of an enveloped RNA virus, simulates a phospholipid bimolecular layer on the surface of the virus or further simulates the surface activity of the viral membrane protein, has the characteristics of concrete structure and physical and chemical properties of the enveloped RNA virus, and has no replication capacity and infectivity. An enveloped RNA virus nucleic acid detection reference / standard is packaged from eukaryocytes by a recombinant virus technology; the platform can ensure that an exogenous target RNA sequence is carried inside reference / standard particles by a genetic manipulation technology; and the reference / standard has a virus envelope protein which is the same as that of a target virus as well as the viral structural characteristics and topological characteristics which are close to those of the target virus, and can truly reflect the influence of various physical and chemical environment factors on the target virus.

Description

Technical field [0001] The invention belongs to the field of biotechnology inventions. The present invention specifically relates to an enveloped RNA virus nucleic acid detection reference product / standard product platform, which mainly consists of a vector carrying an exogenous target sequence and a vector containing MLV gag-pol elements, including or excluding viral membrane proteins. It is produced by transfecting the reference product production cell line. It is a pseudo-virus with enveloped RNA virus, which simulates the phospholipid bilayer on the surface of the virus or further simulates the surface activity of the viral membrane protein. It has a specific enveloped RNA virus Structure and physicochemical characteristics, non-replicating ability, non-infectious. The reference product / standard product for nucleic acid detection with enveloped RNA virus is packaged from eukaryotic cells through recombinant virus technology. The platform can make the reference product / stand...

AI Technical Summary

Problems solved by technology

[0005] However, for different types of viral pathogens, the differences in their biological and physiological structural characteristics lead to differences in resistance to the outside world

Especially for enveloped RNA viruses, since the envelope of enveloped viruses is a lipid bilayer, their RNA is neither exposed nor protected by capsid proteins, and it is topologically impossible to be exposed to naked RNA or coat proteins. Capsid virus phage mimics, therefore simply using naked RNA or capsid virus phage as a reference in the detection of enveloped RNA viruses is inappropriate

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