Pseudovirus based on Vasohibin gene and preparation method and application thereof

A pseudovirus and gene technology, applied in the field of genetic engineering, can solve problems such as unfavorable efficient transfection of target genes, and achieve the effects of inhibiting tumor angiogenesis, improving safety, and inhibiting adhesion and migration.

Inactive Publication Date: 2010-01-13
ARMY MEDICAL UNIV
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Problems solved by technology

However, HPV16L1VLP has strong immunogenicity, and can induce the body to produce high-titer neutralizing antibodies when immunizing animals, which is not conducive to efficient transfection of target genes into target cells

Method used

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  • Pseudovirus based on Vasohibin gene and preparation method and application thereof
  • Pseudovirus based on Vasohibin gene and preparation method and application thereof
  • Pseudovirus based on Vasohibin gene and preparation method and application thereof

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Embodiment Construction

[0033] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. The experimental method that does not indicate specific conditions in the preferred embodiment is usually according to conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, etc., Science Press, 2002) conditions, or as recommended by the manufacturer.

[0034] In a preferred embodiment, firstly, three tandem RGD peptides are inserted between the 266th and 267th amino acids of HPV16L1 to construct the virus capsid chimeric protein HPV16L1-RGD (of course, only one RGD peptide can also be inserted, or Inserting 2, 4 or more RGD peptides in series can achieve the purpose of the present invention); secondly, the Vasohibin gene is cloned into the eukaryotic expression vector to construct the Vasohibin gene recombinant eukaryotic expression vector; fina...

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Abstract

The invention discloses pseudovirus based on Vasohibin gene and a preparation method and application thereof. The pseudovirus is a substance simulating a natural virus structure, which is obtained in such a way that viral capsid chimeric protein HPV16L1-RGD wraps a Vasohibin gene recombination eukaryotic expression vector, wherein the HPV16L1-RGD is formed by inserting RGC peptide between the 266th amino acid and the 267th amino acid of the HPV16L1. The preparation method of the pseudovirus comprises three steps: constructing the HPV16L1-RGD, constructing the Vasohibin gene recombination eukaryotic expression vector and wrapping the Vasohibin gene recombination eukaryotic expression vector with the HPV16L1-RGD. The pseudovirus can escape from organic cleaning mechanism at the maximum, be exclusively absorbed by new vascular endothelial cells of a tumor and tumor cells, play the roles of inhibiting tumor neovascularization and inducing the apoptosis of the tumor cells, and can be used for preparing antineoplastic.

Description

technical field [0001] The invention relates to the field of genetic engineering, in particular to a pseudovirus based on Vasohibin gene and its preparation method and application. Background technique [0002] Tumor is a disease with high morbidity and high mortality that seriously threatens human health. After surgery, radiotherapy and chemotherapy, biological therapy has become an important means in the comprehensive treatment of tumors. At present, tumor biological treatment strategies include targeted therapy of tumor-related genes or tumor suppressor genes, immunotherapy of tumor-associated antigens, etc., but due to technical maturity, effectiveness, safety and economics, etc., they are restricted in practical application. Very restrictive. [0003] Studies have found that more than 90% of human tumors are solid tumors, and the growth and metastasis of solid tumors require angiogenesis, and inhibiting angiogenesis can effectively inhibit tumor growth and metastasis ...

Claims

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Application Information

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IPC IPC(8): C12N7/01C12P21/02C12N15/85A61K48/00A61P35/00
Inventor 杨曌吴玉章
Owner ARMY MEDICAL UNIV
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