Virus structure-imitated high-polymer vesica as well as preparation method and application thereof

A polymer vesicle and polymer solution technology, which is applied to medical preparations with non-active ingredients, medical preparations containing active ingredients, and pharmaceutical formulas, can solve the problems that polymer vesicles have not been publicly reported, and achieve High production efficiency, simple and feasible process, good repeatability

Active Publication Date: 2016-06-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many kinds of polymer vesicles imitating viral nucleocapsids, but there are no public reports on polymer vesicles with surface spikes

Method used

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  • Virus structure-imitated high-polymer vesica as well as preparation method and application thereof
  • Virus structure-imitated high-polymer vesica as well as preparation method and application thereof
  • Virus structure-imitated high-polymer vesica as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] (1) Take PEG (PEG-5000, that is, PEG with a molecular weight of 5000Da), lactide (the molar ratio of PEG to lactide is 1:0.3) and stannous octoate (the amount of stannous octoate is PEG-5000 quality 0.1%) were added together to toluene, stirred at 120°C for 18h at a constant temperature under a nitrogen atmosphere (350r / min), then precipitated in excess cold ether, filtered with suction, and vacuum-dried at 55°C for 4h to obtain PEG 5000 -b-PLA 2000 block copolymers;

[0058] (2) Change the addition amount of PEG and lactide, that is, the molar ratio of PEG-5000 and lactide is 1:3 to obtain PEG 5000 -b-PLA 45000 block copolymers;

[0059] (3) Weigh 0.05g block copolymer PEG 5000 -b-PLA 2000 and 0.05 g block copolymer PEG 5000 -b-PLA 45000 Added in 1g of DCM, mechanically stirred for 6min (stirring speed is 750r / min), to obtain a polymer solution (the amount of the block copolymer is 10wt% of the organic solvent);

[0060] (4) Add the polymer solution to 6.6 g of...

Embodiment 2

[0062] (1) Dissolve PEG-5000 in toluene, add stannous octoate (the amount of stannous octoate is 0.1% of the mass of PEG-5000), under the protection of argon, slowly add caprolactone monomer (PEG-5000 and caprolactone The molar ratio is 1:1), stirred at 110°C for 48h at a constant temperature (300r / min), and the toluene was removed by rotary evaporation under reduced pressure, precipitated in excess cold ether, filtered by suction, and vacuum-dried at 55°C for 4h to obtain PEG 5000 -b-PCL 5000 block copolymers;

[0063] (2) Take PEG (PEG-5000), lactide (the molar ratio of PEG-5000 and lactide is 1:5) and stannous octoate (the amount of stannous octoate is 0.1% of the mass of PEG-5000) and add them together into toluene, stirred at 120°C for 18h under a nitrogen atmosphere (350r / min), then precipitated in excess cold ether, filtered with suction, and dried in vacuum at 55°C for 4h to obtain PEG 5000 -b-PLA 95000 block copolymers;

[0064] (3) Weigh 0.05g block copolymer PEG...

Embodiment 3

[0067] (1) Dissolve PEG-5000 in toluene, add stannous octoate (the amount of stannous octoate is 0.1% of the quality of PEG-5000), under the protection of argon, slowly add caprolactone monomer (PEG and caprolactone molar ratio 1:0.4), stirred at 110°C for 48h (rotating speed: 300r / min), removed toluene by rotary evaporation under reduced pressure, precipitated in excess cold ether, filtered, and dried in vacuum at 55°C for 4h to obtain PEG 5000 -b-PCL 2500 block copolymers;

[0068] (2) Change the addition of PEG and caprolactone, that is, the mol ratio of PEG and caprolactone is 1: 3 to obtain PEG 5000 -b-PCL 45000 block copolymers;

[0069] (3) Weigh 0.05g block copolymer PEG 5000 -b-PCL 2500 and 0.05 g block copolymer PEG 5000 -b-PCL 45000 Add to 1g of DCM / petroleum ether (mass ratio 2:3), mechanically stir for 20min (stirring speed is 200r / min), to obtain a polymer solution (the amount of block copolymer is 10wt% of the organic solvent);

[0070](4) Vacuum-dry the...

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Abstract

The invention belongs to the field of high polymer materials and discloses a virus structure-imitated high-polymer vesica as well as a preparation method and application thereof. The virus structure-imitated high-polymer vesica is prepared from different amphipathic block copolymers through self-assembling or is prepared from amphipathic block copolymers and water-soluble polymers through self-assembling. The virus structure-imitated high-polymer vesica comprises a capsid membrane structure and a spike structure on the surface of a membrane, wherein the inner layer and the outer layer of the capsid membrane structure consist of hydrophilic chain segments in the amphipathic block copolymers or the water-soluble polymers, and an intermediate layer between the inner layer and the outer layer consists of hydrophobic chain segments in the amphipathic block copolymers. The high-polymer vesica has a virus-imitated specific structure and is controllable in structure, high in repeatability, capable of carrying drugs, good in biocompatibility and easy to be phagocytized by cells; the operation of the method is simple, and the production efficiency is high; and meanwhile, the high-polymer vesica can be applied to the relevant fields of high-efficiency nano-carriers, controlled release of medicines and cell imaging.

Description

technical field [0001] The invention belongs to the field of polymer materials, and relates to a polymer vesicle with imitation virus structure, in particular to a polymer vesicle with imitation virus nucleocapsid and surface spike structure, a preparation method and application thereof. Background technique [0002] As the simplest type of non-cellular microorganisms, viruses are mainly composed of nucleic acid molecules and protein capsids; their basic structure has a core (nucleic acid) and a capsid (protein or lipoprotein), both of which constitute the nucleocapsid. The unique capsid structure of the virus can shield potential damaging factors in the internal circulation environment, such as nucleases, pH, etc., and has the function of protecting genetic material. On the surface of some virus particles (especially on the surface of enveloped virus particles), there are radially arranged protrusions, which are called spikes (also called envelope protrusions). Some of the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08L67/04C08L71/02C08L53/00C08L77/00A61K9/127A61K47/34A61K49/00A61K31/704A61P35/00C08J3/00A61K47/32
CPCA61K47/34A61K49/0086A61K9/1273A61K31/704C08J3/00C08L53/00C08L67/04C08L71/02C08J2367/04C08J2371/02C08J2353/00C08L77/00
Inventor 王林格李卫昌廖国行
Owner SOUTH CHINA UNIV OF TECH
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