Process for preparing subunit vaccine of reovirus antigen for grass carp
A reovirus and subunit vaccine technology, applied in the field of virology, can solve the problems of limited application and promotion value, and achieve the effects of high packaging volume, convenient operation, and exclusion of genetic contamination
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[0012] The grass carp reovirus antigen subunit vaccine provided by the invention comprises:
[0013] (1) CIK cells (grass carp kidney cell line) were used for virus propagation, and the virus suspension of grass carp reovirus Hunan Shaoyang strain GCRV873 infected with CIK cells was obtained.
[0014] (2) Purify and concentrate the cell virus suspension infected with GCRV873 by centrifugation.
[0015] (3) The purified virus is treated with a surfactant to disintegrate the complete virus particle structure to form viral nucleic acid and capsid protein subunit components. Surfactant can adopt NP40 (Fluka company product); Its concentration is 0.5%~1%, can make virus capsid protein become loose like this and will not degrade completely.
[0016] (4) Nuclease is used for digestion to degrade the free viral genome dsRNA in the GCRV873 subunit preparation.
[0017] (5) A nucleic acid-free grass carp reovirus antigen subunit protein preparation is obtained by dialysis treatment. ...
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