Lentivirus dissolution buffer solution and application thereof

Abstract

The invention discloses a lentivirus dissolution buffer solution and application thereof. The lentivirus dissolution buffer solution comprises 3-10 mM of Tris, 15-35 mM of proline, 1-5 mM of MgCl2, 5-20% of lactose, 0.005-0.02% of Tween 80 and the balance of water, the percentage is mass percent, and the pH value of the lentivirus dissolution buffer solution is 7-9. The components of the lentivirus dissolution buffer solution are all pharmaceutic adjuvants, the influence on the lentivirus activity can be avoided, and the virus structure can be stabilized and the virus activity can be enhanced by adding proline into the lentivirus dissolution buffer solution; the Tween80 can be used for preventing protein or virus aggregation; and MgCl2 has the effect of maintaining protein or virus activity, through the combined effect of several components in the formula, the activity and recovery rate of lentivirus during purification can be comprehensively improved, and the transduction efficiency of lentivirus and the positive rate of transduction CAR can be improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a lentivirus dissolution buffer and application thereof. Background technique [0002] Lentivirus is commonly used in Chimeric Antigen Receptor T-Cell Immunotherapy (CAR-T), which has the advantages of high transduction efficiency, integration of T cell genome and continuous expression of target proteins. Nowadays, CAR-T production is mostly based on lentiviral vector-mediated CAR gene transfer. However, lentivirus is an extremely fragile viral vector, which is greatly affected by shear force, extrusion, temperature, pH, ionic strength, and handling time during the production process; in addition, lentivirus is relatively easy to Aggregation, which poses a great challenge to lentivirus production and storage. [0003] In the prior art, the buffer usually used for lentivirus dissolution is PBS buffer, 50 mM Tris buffer, PBS+4% lactose buffer, DMEM+2%HSA buffer, HATM buffer or HSTM b...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a lentivirus lysis solution that can solve the aforementioned problems in view of the poor effect of the existing lentivirus dissolution buffer described in the background technology and the inability to provide a more suitable dissolution environment for the lentivirus. buffer

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