The invention discloses carbon
quantum dot nano-materials and a preparation method and application of the carbon
quantum dot nano-materials. The method comprises the following steps: taking
citric acid, mercaptoethylamine and polyethyleneimine, dissolving in
ultrapure water, stirring uniformly, carrying out ultrasonic treatment, at the temperature of 120 to 170 DEG C, performing
microwave heating, dialyzing a mixed subjected to
reactive synthesis in
ultrapure water through a
dialysis bag, then
drying by a vacuum rotary dryer, and collecting materials to obtain the nano-materials. According to the invention, mercaptoethylamine and
citric acid are hydrothermally synthesized with polyethyleneimine to obtain
carbon quantum dots with multiple biological functions under
microwave controlled
auxiliary heating conditions, thus the
carbon quantum dots with characterization performances of different materials of high brightness
fluorescence or high charges and the like can be obtained, and
cell wall fluorescent markers of live
plant cells with high aqueous-phase dispersion and low
cytotoxicity, or materials which promote
endocytosis of animal and
plant cells and realize high-efficient absorption of external solutions, or materials of
plant intact cells realizing the delivery of
nucleic acid transfection and with
cell walls are optimally selected based on changing synthesis conditions according to needs of different biological applications.