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Carbon quantum dot nano-materials and preparation method and application thereof

A nanomaterial and carbon quantum dot technology, applied in the field of organic carbon quantum dot synthesis, can solve problems such as limiting the application of PEI, and achieve the effect of simple and efficient synthesis method, favorable for cell wall diffusion, and small particle size

Active Publication Date: 2017-03-15
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to the existence of these shortcomings, the application of PEI is limited

Method used

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  • Carbon quantum dot nano-materials and preparation method and application thereof
  • Carbon quantum dot nano-materials and preparation method and application thereof
  • Carbon quantum dot nano-materials and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Dissolve 0.15 g of citric acid, 0.13 g of mercaptoethylamine, and 0.1 g of polyethyleneimine (Mw = 800) in 3 mL of ultrapure water, stir well, and ultrasonically (power 200 W, frequency 28 KHz) for 3 min at 170 ℃, heated with a single-mode microwave synthesizer (power 500 W) for 10 min, and the synthesized mixture was dialyzed in ultrapure water at 45 °C for 36 h through a dialysis bag (Mwco: 1000), and the ultrapure water was changed every 10 h. The material was collected after drying in a vacuum rotary dryer, and the fluorescence emission was detected with a UV lamp (305 nm, 365 nm), such as figure 1 As shown, the synthesized material is transparent brown-yellow under white light, and has high-brightness fluorescence emission under ultraviolet light (305 nm, 365 nm). Malvern particle size analyzer detects the DLS particle size and Zeta potential value of the synthetic material. The particle size is about 0.7 nm, which is relatively small; it is positively charged, abo...

Embodiment 2

[0041] Disperse 20 μL of the material synthesized in Example 1 in the water system, add 10 μg of the plasmid into ultrapure water, make up the total amount to 50 μL, and ultrasonically shake in an ice bath for 3 minutes in an ultrasonic cleaner, and then put it in a dark place to combine After 30 min, add it to the single cells of Liriodendron tulipifolius suspension that was sieved through a 300-mesh cell sieve, mix well, then transfer to a cell culture plate, add 1 mL of ultrapure water, and observe the fluorescent carbon dots suspended in Liriodendron tulipifera after 24 hours. distribution in lineage cells. It can be seen from Example 1 that the synthetic material has certain positively charged groups, which can adsorb plasmids through electrostatic interaction, and co-incubating with cells can adsorb cell walls, such as Figure 12 As shown, the GFP-adsorbed green fluorescent protein expression plasmid can be delivered into the cell for expression, so that there is a high-...

Embodiment 3

[0043] Using different ratios of reaction substrates, the materials were synthesized by a microwave synthesizer at different reaction temperatures for 10 minutes. 0.15 g citric acid, 0.08 g mercaptoethylamine, and 0.2 g polyethyleneimine (Mw = 800) were dissolved in 3 mL of ultrapure water, stirred and mixed, ultrasonicated for 3 min, heated at 150 °C for 10 min in a microwave synthesizer, and the synthesized mixture was dialyzed in ultrapure water at 45 °C for 36 h through a dialysis bag (Mwco: 1000). The ultrapure water was changed every 10 h, and the material was collected after drying in a vacuum rotary dryer. 200 μL was taken and 1 mL of single cells of Liriodendron tulipifera suspension were co-incubated in 3 mL of ultrapure water for 24 h. Material-specific fluorescence found that plasmolysis appeared in the cells treated with the synthesized sample, and multiple endocytic vesicles with specific fluorescence distributed on the inner side of the cell membrane appeared in ...

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Abstract

The invention discloses carbon quantum dot nano-materials and a preparation method and application of the carbon quantum dot nano-materials. The method comprises the following steps: taking citric acid, mercaptoethylamine and polyethyleneimine, dissolving in ultrapure water, stirring uniformly, carrying out ultrasonic treatment, at the temperature of 120 to 170 DEG C, performing microwave heating, dialyzing a mixed subjected to reactive synthesis in ultrapure water through a dialysis bag, then drying by a vacuum rotary dryer, and collecting materials to obtain the nano-materials. According to the invention, mercaptoethylamine and citric acid are hydrothermally synthesized with polyethyleneimine to obtain carbon quantum dots with multiple biological functions under microwave controlled auxiliary heating conditions, thus the carbon quantum dots with characterization performances of different materials of high brightness fluorescence or high charges and the like can be obtained, and cell wall fluorescent markers of live plant cells with high aqueous-phase dispersion and low cytotoxicity, or materials which promote endocytosis of animal and plant cells and realize high-efficient absorption of external solutions, or materials of plant intact cells realizing the delivery of nucleic acid transfection and with cell walls are optimally selected based on changing synthesis conditions according to needs of different biological applications.

Description

technical field [0001] The invention belongs to the technical field of synthesis of organic carbon quantum dots with biological functions, and relates to a method for preparing fluorescent polymers or carbon dots synthesized by microwave controllable auxiliary heat to promote mercaptoethylamine-tuned citric acid and polyethyleneimine, in particular to a carbon dot Quantum dot nanomaterials and their preparation methods and applications. Background technique [0002] With the rapid development of genome high-throughput sequencing technology, researchers have accumulated genome sequences of many species, and the research on these species has also entered the era of functional genomes. Among them, many genes predicted by bioinformatics need to be studied for functional identification and functional interaction. Therefore, a variety of novel and precise gene function research technologies are emerging, such as gene editing technology, RNA interference, gene site-directed mutatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/65C01B32/15B82Y40/00B82Y30/00B82Y20/00C12N15/87G01N21/64
CPCC01P2002/80C01P2002/84C01P2004/64C09K11/65C12N15/87G01N21/6486
Inventor 施季森陈桢雨夏兵陈金慧霍爱玲王宾
Owner NANJING FORESTRY UNIV
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