84 results about "Lignin-modifying enzyme" patented technology

The invention discloses a lignin degradation solution and a preparation method thereof as well as a method for degrading lignin by using the lignin degradation solution, and belongs to biochemical and bio-refinery methods to solve the problem that the efficient biodegradation of lignin is difficult to realize by adopting single ligninase of laccase at present. The lignin degradation solution is prepared by the following steps: dissolving laccase and manganese peroxidase into an acetic acid-sodium acetate buffer solution of which the pH value is 4-6 in an enzyme load ratio of (10:1)-(1:5) to ensure that the enzyme loads of the laccase and manganese peroxidase are respectively 1U/ml-50U/ml and 1U/ml-50U/ml; and then adding 1-10mM of MnSO4 and 0.1-1mM of H2O2, wherein the laccase and manganese peroxidase are obtained by fermenting white rot fungi to obtain extracellular crude enzyme liquid and then performing separation and purification respectively. According to the lignin degradation solution and the methods disclosed by the invention, the synergistic oxidative degradation of macromolecular lignin with rich structural diversity is realized, and compared with a degradation reaction system with single ligninase, the degradation rate of the macromolecules of the lignin can each 30-50%, the degradation conversion efficiency is significantly improved, and the degradation solution and the methods can be applied to the fields of bio-refinery of lignocelluloses, biological pulping or environmental treatment and the like.

The invention discloses a production method of a complex enzyme liquid for chemi-mechanical pulping. The production method is characterized by comprising the following steps: weighing various component enzymes, mixing and compounding the various component enzymes in a compounding tank, preventing corrosion, inspecting, packaging and storing in a storeroom at 5 DEG C, wherein the various component enzymes comprise alkaline pectinase, xylanase, mannose and ligninase. The application of the complex enzyme liquid in chemi-mechanical pulping is realized as follows: before steaming paper-making raw materials, adding the complex enzyme liquid to the paper-making raw materials through a metering pump, uniformly mixing the complex enzyme liquid with the paper-making raw materials for enzymatic hydrolysis under enzymatic treatment conditions that the temperature is 40-60 DEG C and enzymatic hydrolysis duration is 30-240 minutes; after the enzymatic hydrolysis, performing following processes as normal. The production method, through coupling synergistic effect of multiple enzymes of the complex enzyme liquid in chemi-mechanical pulping, can be used for improving the whiteness of pulp by 2-5% and reducing the pulp yellowing under a condition of guaranteeing high yield and low cost of pulping; the production method can be used for reducing the dosage of chemical raw materials by 20-30% and reducing the pulp grinding energy consumption by 18-30%; meanwhile, the production method can be used for reducing pollution and improving the pulp quality.

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

The invention provides a nano carbon organic compound fertilizer. The nano carbon organic compound fertilizer is composed of following components according to suitable weight parts: urea, monopotassium phosphate, hairs, palygorskite, dimethyl sulfoxide, a boron fertilizer and zinc fertilizer mixture with the weight ratio of 1:0.5, ferrous sulfate, nodule bacteria, azotobacter chroococcum, nano carbon, rhizoma curculiginis, pseudolarix kamepferi, radix sophorae flavescentis, ligninase, plant ash, humic acid, dicyandiamide, animal dung and powdery EDTA (Ethylene Diamine Tetraacetic Acid) chelated manganese. The nano carbon organic compound fertilizer can be good for effective utilization of secondary elements and microelements, is suitable for crops including wheat, rice, corns, soybeans, oilseed rapes and the like, and can enhance the cold-resisting, drought-resisting, and disease and insect pest-resisting functions; the nano carbon organic compound fertilizer can be used on cotton and linen crops to enhance the tension and the strength of fibers and the yield and the quality are obviously improved.

In some variations, the invention provides a process for producing purified cellulose, comprising: providing a feedstock comprising lignocellulosic biomass; contacting the feedstock with sulfur dioxide, water, and a solvent for lignin, to produce intermediate solids and a liquid phase comprising hemicelluloses and lignin; mildly bleaching the intermediate solids to further delignify the intermediate solids, thereby generating cellulose-rich solids; and washing the cellulose-rich solids to generate purified cellulose with less than 2 weight percent lignin. The bleaching may employ bleaching agents including lignin-modifying enzymes. The bleaching and washing steps may be combined. It is also possible to carry out bleaching prior to, or simultaneously with, biomass fractionation in the digestor, which may help reduce downstream lignin precipitation. The purified cellulose may be utilized for making cellulose materials or cellulose derivatives, or for hydrolysis to produce glucose.

The invention discloses a ligninase-assisted process for degumming China hemp fiber by freezing and suddenly heating. The ligninase-assisted process for degumming China hemp fiber by freezing and suddenly heating comprises the following steps: ultrasonically poaching and pre-pickling; then carrying out UV radiation, freezing and suddenly heating, and processing by the ligninase; and finally degumming at high temperature and high pressure. The gumming effect of the surface of the China hemp fiber processed by the process is superior than that of a chemical method and a mechanical method; the China hemp fiber processed by the process is relatively low in residual gun rate and content of ligninase, so that the requirements of a textile finishing process can be met; good whiteness and softness are obtained; meanwhile, the breakage strength of the fiber is controlled in the spinning requirements; the length and the fineness of the fiber are also improved; the comfortable property is greatly improved; the use amount of chemical treatment reagents can be reduced; the polluted wastewater discharge is relatively low; the ligninase-assisted process for degumming China hemp fiber by freezing and suddenly heating is a green and environment-friendly process.

The invention discloses a production method of a compound enzyme liquid used in the papermaking and pulping process of wheat straws and rice straws. The production method is characterized by comprising the steps of weighing each component enzyme, mixing and compounding all the component enzymes in a compounding pot, performing corrosion prevention, inspecting, packaging, and storing in a warehouse with the temperature of 5 DEG C, wherein the component enzymes are alkaline pectinase and xylanase, or are alkaline pectinase, xylanase, mannose and ligninase. The application of the compound enzyme liquid in the papermaking and pulping process of wheat straws and rice straws comprises the following steps: papermaking raw materials are pre-treated and then sent into a digester for enzyme treatment, namely the raw materials are soaked with the compound enzyme liquid, wherein the enzyme treatment conditions are as follows: the temperature ranges from 40 DEG C to 60 DEG C, the mass ratio of an absolute dry raw materials to the compound enzyme liquid is 100:(1.2-1.8) and the enzyme soaking time ranges from 20 minutes to 60 minutes; after enzyme treatment, the papermaking raw materials are digested in the digester. Each component enzyme in the compound enzyme liquid is high in activity but has no activity to crystalline cellulose and carboxymethyl cellulose; and compared with the traditional chemical pulping method, the production method has the advantages that the use amount of alkali is lowered by about 50%, the content of COD (Chemical Oxygen Demand) in waste water is lowered by over 30%, the production cost is lowered by about 20% and the quality of paper products is enhanced.

The invention relates to a boiling assistant for preparing high polymerised pulp. The assistant comprises 30-50% of sodium sulfite, 5-10% of alpha-methylanthraquinone (alpha-MAQ), 5-15% of lipase, 1-10% of hemicellulase, 1-10% of ligninase, 10-20% of diethylene triaminepentaacetic acid (DTPA) and 20-40% of sodium dodecyl benzene sulfonate. The assistant can promote the swelling of celluloses, quickly improve the permeation of alkaline liquor on celluloses, accelerate the breakage of primary walls, quickly dissolve out lignin, pectin, wax and other non-fiber impurities, improve the boiling reaction speed, oxidize the reducing groups (aldehyde terminal groups) of the cotton linter carbohydrate into various alkali-stable sugar acid terminal groups, inhibit the peeling reaction, reduce the degradation of celluloses and improve the yield.

The invention discloses a method for producing ligninolytic enzymes, and belongs to the biotechnical field. The method comprises the following steps: 1, respectively cloning lignin peroxidase, manganese peroxidase and laccase genes from microbes; 2, constructing a Pichia pastoris expression vector containing the expression cassettes of the above three genes, and a Saccharomyces cerevisiae expression vector containing the expression cassettes, and converting the above vectors to corresponding host bacteria to obtain engineering bacteria; and 3, respectively fermenting Pichia pastoris expression engineering bacteria and Saccharomyces cerevisiae engineering bacteria to produce recombinant lignin peroxidase, manganese peroxidase and laccase. The method has the following advantages: engineering bacteria are used to substitute natural bacterial strains to produce the lignin peroxidase, manganese peroxidase and laccase, so the enzyme outputs are improved through increasing the copy numbers of the genes; and the engineering bacteria containing three enzyme gene expression cassettes substitute engineering bacteria containing a single enzyme gene expression cassette to produce single enzymes, so the raw material, the energy consumption and the manpower resource are saved.

The invention provides a spent bleaching clay-based bio-organic fertilizer, the raw material formula of which includes the following raw materials in parts by weight: 50-70 parts of spent bleaching clay, 10-15 parts of humic acid, 0.5-2 parts of composite bacteria liquid, 0.5-1 part of complex enzyme, 5-10 parts of magnesium aluminum silicate and 0.01-0.1 part of plant growth regulator, wherein the composite bacteria liquid is prepared by mixing pseudomonas, bacillus subtitles, white rot fungi, bacillus cereus, and bacillus thuringlensis according to the volume ratio of nutrient solution being 1 to (1.3-1.6) to (0.5-1.1) to (0.3-0.6) to (0.2-0.4); the complex enzyme is formed by mixing amylase, lipase and ligninase according to the mass ratio of 1 to (1.4-1.6) to (0.3-0.8); and the plant growth regulator is a combination of brassinolide with one or more of cytokinin, thidiazuron, gibberellic acid, indoleacetic acid and naphthalene acetic acid. The bio-organic fertilizer has multiple functions of preventing soil pests, degrading pesticide and chemical residuals in soil, curing and stabilizing heavy metal ions in the soil, is excellent in comprehensive modification effect, has no root burning and seedling burning problems and is low in cost.

A method for rapid, high throughput screening of the activities of enzymes, especially ligninases and its enzyme cocktails, using nanostructure initiator mass spectrometry (NIMS) surfaces, substrates and methodology.