Production method and application of complex enzyme liquid for chemi-mechanical pulping
A technology of chemical machinery and compound enzyme liquid, which is applied in the direction of biochemical equipment and methods, oxidoreductase, and microbial/enzymatic cellulose treatment, etc., which can solve the problem of single enzyme, yellowing of pulp, high energy consumption and equipment wear and other issues, to achieve the effect of improving quality, reducing energy consumption and reducing pollution
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Embodiment 1
[0015] A production method of a compound enzyme solution for chemical mechanical pulping. The enzymes of each component are weighed, mixed and prepared in a compound tank, preserved, inspected, packaged, and then stored in a warehouse at 5°C. The enzymes of each component are alkali Alkaline pectinase, xylanase, mannanase and ligninase; the alkaline pectinase is CCTCC NO: M 2010004 bacterial strain culture, shake flask fermentation, seed tank to fermenter, filter , degerming, and antiseptic treatment; the xylanase is prepared after CGMCC NO:5466 strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, and antiseptic treatment; the nectar Glycanase is prepared by CGMCC NO: 6226 strain cultured by strain, shake flask fermentation, seed tank to fermenter, filtered, sterilized, and preserved; the ligninase is prepared by CGMCC NO: 6225 strain by strain Cultivate, shake flask fermentation, prepare from seed tank to fermenter, filter, sterilize, a...
Embodiment 2
[0060] The activity of alkaline pectinase in the compound enzyme liquid described in this embodiment is 1300u / ml, the activity of xylanase is 1500u / ml, the activity of mannanase is 800u / ml, and the activity of ligninase is 180u / ml.
[0061] The papermaking raw material described in this embodiment is poplar wood chips.
[0062] The chemical mechanical pulping described in this embodiment is APMP alkaline hydrogen peroxide chemical mechanical pulping.
[0063] In this example, the addition amount of the compound enzyme liquid is 1.5% of the mass percentage of the absolute dry papermaking raw material. The enzyme treatment conditions are: temperature 50°C, pH 8.0, and enzymolysis time 230 minutes. After the enzymolysis is completed, the follow-up process is carried out as usual . That is, poplar wood chips are enzymatically hydrolyzed, steamed, and DTPA and Na 2 SO 3 Extrusion prepreg, adding NaOH and H in two stages 2 o 2 Extrusion prepreg, adding NaOH and H in three sta...
Embodiment 3
[0067] The activity of alkaline pectinase in the compound enzyme liquid described in this embodiment is 1200u / ml, the activity of xylanase is 1300u / ml, the activity of mannanase is 700u / ml, and the activity of ligninase is 130u / ml.
[0068] The papermaking raw material described in this embodiment is reed.
[0069] The chemical mechanical pulping described in this embodiment is BCTMP bleached chemical thermomechanical pulping.
[0070] In this example, the amount of compound enzyme liquid added and the mass percentage of absolute dry papermaking raw materials are 1.5%, the enzyme treatment conditions are: temperature 55°C, pH 8.0, enzymolysis time 180 minutes, after the enzymolysis is completed, the subsequent process is carried out as usual . That is, the reed is pulped after enzymatic hydrolysis, steaming, chemical impregnation, preheating, pressure refining, screening, and bleaching.
[0071] After the raw materials are pretreated by the compound enzyme solution, the am...
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