Production method and applications of compound enzyme liquid used in papermaking and pulping process of wheat straws and rice straws
A composite enzyme solution and production method technology, applied in the field of paper pulping, can solve the problems of unfavorable industrial production, difficult to control component damage, and single enzyme species, so as to improve accessibility and utilization rate, reduce COD content, increase The effect of product quality
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Embodiment 1
[0017] A production method of a compound enzyme solution for wheat straw and rice straw papermaking pulping. The enzymes of each component are weighed, mixed and prepared in a compound tank, preserved, inspected, packaged, and stored in a warehouse at 5°C. The enzymes of each component It is alkaline pectinase and xylanase; the alkaline pectinase is CCTCC NO: M 2010004 strain after strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, antiseptic treatment Preparation; the xylanase is prepared by CGMCC NO: 5466 strains through strain culture, shake flask fermentation, seed tank to fermenter, filtration, sterilization, and antiseptic treatment; alkaline pectin in the compound enzyme liquid The enzyme activity is 1500u / ml-2000u / ml, and the xylanase activity is 1000u / ml-1500u / ml.
[0018] The CCTCC NO: M 2010004 bacterial strain described in the present invention is obtained from nearly a hundred bacterial strains by naturally collecting bact...
Embodiment 2
[0044] The various component enzymes described in the present invention are alkaline pectinase, xylanase, mannanase and ligninase; the mannanase is CGMCC NO: 6226 strains cultured by strains and fermented in shake flasks , prepared from seed tank to fermenter, filtered, sterilized, and preservative treated; the ligninase is CGMCC NO: 6225 strain, cultured by strain, fermented by shake flask, transferred from seed tank to fermenter, filtered, sterilized, preserved Prepared after treatment; the activity of alkaline pectinase in the compound enzyme liquid is 1000u / ml~1500u / ml, the activity of xylanase is 800u / ml~1200u / ml, and the activity of mannanase is 800u / ml ml~1200u / ml, the activity of ligninase is 20u / ml~80u / ml.
[0045]The CGMCC NO: 6226 bacterial strain described in the present invention is collected in Ningxia, China. During the strain selection and screening process, a high-yield bacterial strain with a laboratory number of PARK9639 was screened out. According to the ma...
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