Straw degradation actinomycete and application thereof

A technology of liberating threads and straws, applied in the direction of bacteria, microorganisms, biochemical equipment and methods, etc., can solve the problems of less research and application of actinomycetes, and achieve the effects of easy industrial implementation, low cultivation cost, and convenient operation

Active Publication Date: 2012-10-03
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on straw degrading microorganisms mainly focuses on bacteria and fungi, such as Bacillus, Penicillium, Trichoderma, Aspergillus niger, Fusarium, etc., but there are few studies and applications on actinomycetes.

Method used

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  • Straw degradation actinomycete and application thereof
  • Straw degradation actinomycete and application thereof
  • Straw degradation actinomycete and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Isolation, purification and preservation of embodiment 1 Streptomyces griseorubens (Streptomyces griseorubens)

[0025] Take rotten straw and soil from the paddy field of Jiaoda Pujiang Base, Pujiang Town, Minhang District, Shanghai as samples, and adopt the method of shake flask enrichment culture. After the different samples are ground and mixed, take 10g, put them in 100mL enrichment medium, and keep at 30℃ , 150rpm constant temperature culture. Afterwards, every 5 days, 10% of the inoculum was transferred to another enriched medium, and the acclimatization was repeated 4 times. The enrichment medium adopts the medium with CMC-Na as the sole carbon source.

[0026] Take 100 μL of the enrichment solution and spread it on the CMC-Congo red medium plate, such as figure 2 As shown, place it in a constant temperature and humidity incubator at 30°C and culture it upside down, and a transparent hydrolysis circle will appear after 5 days. Pick a small amount of bacterial...

Embodiment 2

[0028] Identification of Example 2 Streptomyces griseorubens (Streptomyces griseorubens)

[0029] On November 28, 2011, the China Industrial Microbiology Collection Center used the internationally recognized identification method for actinomycetes - multiphase identification to identify the JSD-1 bacteria at the genus level. Identification included observation of microbial morphological characteristics, experiments on physiological and biochemical characteristics, and phylogenetic analysis of 16S rDNA sequences. The bacteria were identified as Streptomyces griseorubens.

[0030] The Streptomyces grisea was named JSD-1, and it was preserved in the General Microorganism Center (CGMCC) of China Committee for Microbial Culture Collection (CGMCC) on January 9, 2012. Address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing , Chinese Institute of Microbiology, deposit number is CGMCC No.5706.

Embodiment 3

[0031] Example 3 Test of Cellulase, Ligninase, Pectinase and Hemicellulase Production Ability of Streptomyces grisea of ​​the present invention

[0032] First, use the corresponding enzyme detection plate to measure the enzyme production of the bacteria. Congo red plate can be used for the detection of cellulose, hemicellulose and pectin, while the three lignin degrading enzymes need guaiacol, aniline blue, etc. to test.

[0033] After the test is completed, the DNS method is used to measure the activities of the two cellulase CMCase and FPase in the fermentation broth, which are 55.69U / ml and 21.23U / ml respectively; the DNS method is also used to measure the activities of pectinase and xylanase 3.66U / ml and 4.27U / ml. Wherein, one unit of enzyme activity is the amount of enzyme that generates 1 μg of monosaccharide product per milliliter of enzyme solution catalyzing reactants per minute.

[0034] ABTS, veratrol, Mn 2+ As the reaction substrate, the activities of laccase (L...

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Abstract

The invention relates to the technical field of straw degradation, in particular to a straw degradation actinomycete and application thereof. The straw degradation actinomycete belongs to streptomyces griseorubens (Streptomyces griseorubens), and the preservation serial number is CGMCC N0.5706. Compared with the prior art, the straw degradation actinomycete and the application have the advantagesthat the streptomyces griseorubens has strong capacity for producing cellulose, hemicellulase, pectinase and ligninase, is free of negative effects on carbon cycling of the nature, and is free of damage to the environment; the streptomyces griseorubens has strong straw degradation capability, enables straw weightlessness rate to achieve 76% after staying in a straw degradation culture medium for 30 days, and is low in culture cost, convenient to operate and easy to industrially apply; and the streptomyces griseorubens has certain antagonism on plant disease microorganism in the soil.

Description

technical field [0001] The invention relates to the technical field of straw degradation, in particular to a straw-degrading actinomycete and its application. Background technique [0002] my country is a large agricultural country, and the annual output of various crop straws is about 700 million tons. Since the late 1990s, with the increase in crop yields, the total amount of straw has increased rapidly. In order to catch up with the farming season, rush to sow seeds, and save trouble, the majority of farmers have begun to burn straw in most areas. Large-scale straw burning not only causes great environmental pollution, but also wastes precious biological resources. [0003] The open-air burning of straw has been widely concerned by the society, and its hazards are mainly reflected in the following three aspects: (1) After burning, a large amount of pollutants such as smoke, dust, carbon monoxide, carbon dioxide, and sulfur dioxide are produced, which deteriorates the qua...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/00C12R1/465
Inventor 支月娥周培冯海玮毛亮
Owner SHANGHAI JIAO TONG UNIV
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