60 results about "KANAMYCIN SULFATE" patented technology

The invention discloses Pseudomonas nitroreducens and application thereof, belonging to the field of environmental microorganism. The Pseudomonas nitroreducens CQ1 of the invention is derived from thesewage of oxidation ponds in the sewage treatment system of Beijing Yanshan Petrochemical Co., Ltd., and obtained by enriching, separating and purifying. The Pseudomonas nitroreducens CQ1 is culturedand collected in the China General Microbiological Culture Collection Center (hereinafter referred to as CGMCC) with the collection number being CGMCC No.3159. The invention further discloses the application of Pseudomonas nitroreducens in the biodegradation of quinoline or derivatives thereof. Quinoline or derivatives thereof with the concentration reaching 700mg / L can be degraded by Pseudomonasnitroreducens of the invention, and the degradability can reach 100% after treating for 72h. Moreover, the Pseudomonas nitroreducens CQ1 of the invention is sensitive to kanamycin sulfate, tetracycline and gentamicin and does not carry plasmids.

The invention discloses an agrobacterium-mediated jatropha curcas gene transformation method. A material after 1 / 3 to 1 / 4 of base is cut from the cotyledon of a mature seed of jatropha curcas is used as an agrobacterium infection receptor. The method comprises the following steps of: after co-culturing, inoculating the transformed receptor and the agrobacterium to a culture medium without a screening agent to perform callus induced culture for 4 weeks; and succeeding the receptor and the agrobacterium to a selective culture medium and screening resistant buds by using kanamycin sulfate as a screening agent, sub-culturing the resistant buds obtained by screening to obtain resistant plants, and obtaining transgenic jatropha curcas plants by molecular detection. Compared with the conventional jatropha curcas transformation method, the method can obtain high regenerative bud induction rate, simplifies the transformation program, shortens the transformation period, greatly improves the transformation efficiency and has good application prospect.

The invention discloses a composition for treating bacterial diarrhea of pigs and a preparation method of the composition. The composition comprises, by weight, 5-15% of ampicillin sodium, 5-20% of kanamycin sulfate, 5-15% of taurine, 5% of potassium chloride, 8% of sodium bicarbonate, 10% of sodium chloride and the balance sodium chloride. The composition for bacterial diarrhea reduces drug resistance of pathogens, takes effect rapidly, and is high in cure rate. Through reasonable matching, the composition combining the medicines has the obvious therapeutic effect on bacterial diarrhea of the pigs, can lower pathogenic drug resistance probability, reduces side effects of the medicines, treats both symptoms and root causes, and speeds up treatment.

The invention discloses a compound sodium sulfadimidine injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating toxoplasmosis of the pig, addresses both the symptoms and root causes, reduces the time and dosage of used drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 sodium sulfadimidine, 1 to 10kg of lincomycin hydrochloride, 5 to 20kg of doxofylline, 1 to 4kg of trimethoprim, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 40kg of propylene glycol, 10kg of 95% alcohol and the balance of water for injection. The injection liquid has fast effect for toxoplasmosis of the pig, addresses both the symptoms and root causes, can effectively treat and prevent secondary infection of bacterium, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound sodium sulfadimidine injection liquid is an injection liquid and has convenient use.

The invention discloses a compound ofloxacin injection for animals for treating postnatal infection and a preparation method thereof. The invention aims to provide a compound ofloxacin injection for the animals which has quick response on treating the postnatal infection of livestock, is effective and efficient and can treat both principal and secondary aspects of diseases, and a preparation method thereof. Each 100L of injection comprises the following components: 0.5 to 10kg of ofloxacin, 3 to 15 of billion units of kanamycin sulfate, 0.06kg of dexamethasone sodium phosphate, 0.02 to 0.1kg of bethanechol chloride, 1,000ml of hydrochloric acid, and the balance of water for injection. The injection has double functions of bacteria inhibition and sterilization by using the ofloxacin in combination with the kanamycin sulfate, reduces the probability that bacteria produce the drug resistance, and increases the sensitivity of the bacteria; in addition, the dexamethasone sodium phosphate has anti-inflammatory, detoxicating and antiallergic functions; and the bethanechol chloride stimulates the intestines and stomach as well as uterine smooth muscle to creep. The product treats both principal and secondary aspects of diseases on the postnatal infection of dams and complicating diseases thereof, and can accelerate the postpartum recovery of the dams.

The invention discloses an external medicinal composition for treating skin ulcers (bedsores, burn ulcers and crural region ulcers) and a preparation method thereof, and belongs to the technical field of medicaments. The compound refined white sugar formulation of the composition of the invention is a medicinal composition which is prepared by mixing the medicinal active ingredients such as refined white sugar, neomycin sulfate (or kanamycin sulfate or povidone iodine) and a medicinal diluent or a carrier. In a skin external medicine administration form, the formulation of the composition can be powder or ointment which is used for treating the skin ulcers such as the bedsores, the burn ulcers, the crural region ulcers and the like.

The invention relates to a lilium tenuifolium efficient genetic transformation system based on somatic embryogenesis. The system comprises the following process steps of embryogenic callus induction through a somatic embryogenesis technology system, screening antibiotic hygromycin and kanamycin sulfate screening at the proper concentration, agrobacterium infection solution preparation, infection and co-culture, resistant callus screening and subculture; resistant somatic embryo germination and seedling forming, transgene plant GUS tissue dyeing and PCR detection. The lilium tenuifolium embryogenic callus is used as a receptor material; the somatic embryo is generated through induction after the genetic transformation for the first time, and is further and directly developed into a completeplant; the conversion rate is obviously improved in comparison with other kinds of lilium tenuifolium in the existing report, and can reach 29.17 percent. The system has the advantages that the recipient cell cardinal number is great; the somatic embryo induced after the conversion is a single cell source; the generation of a chimera is well avoided; the transformation generation exogenous geneshereditary stability is high; the aberration rate is low.

The invention discloses a surface plasmon resonance sensor chip for detecting gram-negative bacteria and a preparation method and application thereof. The chip comprises a glass substrate layer, a gold film layer and a probe molecular layer. The gold film layer is disposed on the glass substrate layer. The probe molecular layer is disposed on the gold film layer. A probe molecule of the probe molecule layer contains at least one of drug molecules of streptomycin sulfate, kanamycin sulfate, gentamicin sulfate, gentamycin-micronomicin, vistamycin sulfate, neomycin, polymyxin B, rifampicin, chloramphenicol and fosfomycin. The invention also discloses a preparation method and application of the chip. The surface plasmon resonance sensor chip creatively realizes detection of gram-negative bacteria based on surface plasmon resonance, has high sensitivity and good selectivity, has a gram-negative bacterium concentration detection linear range of 10-10<6> CFU / mL and can be used for detecting gram-negative bacteria in solutions such as detection water, drinking water or human body fluids.

The invention relates to a method for establishing liver cell lines of semilabeo obscurus. The method includes steps of 1), acquiring the livers of the semilabeo obscurus: disinfecting the bodies of the semilabeo obscurus by methylene blue and alcohol jointly; 2), carrying out primary culture: carrying out culture on the livers by the aid of high-glucose DMEM (dulbecco modified eagle medium) culture fluid with kanamycin sulfate and streptomycin sulfate; 3), carrying out passage culture: replacing the cell culture fluid by basic culture fluid when the sixth generation of livers is subjected to passage culture, and successfully establishing the liver cell lines of the semilabeo obscurus. The method has the advantages that the obtained liver cell lines of the semilabeo obscurus are irregularly polygonal, can be subjected to continuous culture transfer and can be directly applied to research on biological characteristics, and requirements on conservation of resources of semilabeo obscurus species and theoretical research and application can be met.

The invention discloses an antibody reagent preserving fluid which comprises ingredients such as sodium chloride, dipotassium phosphate, sodium dihydrogen phosphate, kanamycin sulfate, bovine serum albumin and SOD(Superoxide Dismutase). All the ingredients in the preserving fluid are matched with each other rationally and advantageous for the preservation of antibodies, in particular monoclonal antibodies for blood grouping, and as a result, the preservation period of the monoclonal antibodies at the normal temperature is longer and can be up to 2 years. The antibody reagent preserving fluid is capable of an appropriate environment for preserving the antibody reagent, and also solves the problem that the antibody reagent cannot be preserved for a long time at the room temperature. The antibody reagent preserved with the antibody reagent preserving fluid is applied to the production of a microcolumn gel immunodetection reagent card so that long-term preservation of the microcolumn gel immunodetection reagent card at the normal temperature can be realized, and therefore, the gel immunodetection reagent card can be widely applied.

The invention provides a fermentation medium and a method for fermenting nicotinamide monoucleotide (NMN) transferase by the same.The method includes the following steps that 1, a solid medium containing Escherichia coli is prepared; 2, activation liquor is prepared; 3, Escherichia coli is inoculated into the activation liquor, and kanamycin sulfate is added to obtain liquor containing activated Escherichia coli thalli; 4, the liquor containing thalli is inoculated into a sterilized fermentation medium for fermentation cultivation, an inductive agent IPTG is added for inductive cultivation, and fermentation liquor containing NMN transferase is collected; 5, the fermentation liquor is centrifuged, sediment is taken, and then Escherichia coli wet thalli containing NMN transferase are obtained.The fermentation medium has simple components, is low in cost and has broad application prospects, and the fermentation method is simple, practical and easy to implement.

The soybean shoot tip transformation vacuum infiltration supplementary exogenous gene introudction method includes the following steps: in the course of soybean shoot tip transformation by using agrobacterium mediated BT gene adopting soybean seed germination after disinfection, removing terminal bud and resulting in epidermal meristematic cell damage as explant, under the condition of vacuum pressure making agroinfection treatment, making co-cultivation to germination culture meidum, using a certain quantity of kanamycin sulfate and adding it into budding culture medium and rooting culture medium to make screening, so can obtain more regenerative seedlings and transformed soybean plants. Said invention can obvious raise agroinection effect and effectively raise budding rate and number oftransformed seedlings.

The invention aims at providing a preparation method of a synergistic tylosin tartrate soluble powder compound medicine. The synergistic tylosin tartrate soluble powder compound medicine has a rapid and definite curative effect on respiratory system infections and complications of livestock and poultry. The preparation method comprises the following steps of: mixing 0.2 part of trimethoprim and 0.2 part of cosolvent, then crushing, and sieving by using a 120 meshes sieve or a finer sieve; and then uniformly mixing with 1 part of tylosin tartrate, 1 part of kanamycin sulfate and 0.05-0.1 part of pain alleviant by an equivalently successive increase method, thus obtaining the synergistic tylosin tartrate soluble powder compound medicine. The cosolvent is citric acid or succinic acid or the mixture of citric acid and succinic acid and the pain alleviant is procaine hydrochloride or lidocaine hydrochloride or the mixture of procaine hydrochloride and lidocaine hydrochloride. The animal synergistic tylosin tartrate soluble powder compound medicine for injection contains three medical components which are tylosin tartrate, kanamycin sulfate and trimethoprim, can be used for solving the problems that the secondary infection cannot be controlled very well during the treatment of mycoplasma infection due to the narrow antibacterial spectrum of the tylosin tartrate, and the trimethoprim is insoluble in water, has a synergistic effect on tylosin tartrate and kanamycin sulfate and has an obvious medicine curative effect.

The invention discloses a compound kanamycin sulfate injection liquid for a pig and a preparation method thereof, aiming at providing a compound kanamycin sulfate injection liquid which has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces the time and dosage of taking drug and has convenient drug usage, and a preparation method with simple technique and easy implementation. Each 100L injection liquid comprises: 5 to 20 billion units of kanamycin monosulfate, 1 to 10kg of lincomycin hydrochloride, 0.1 to 2kg of doxofylline, 0.05kg of dexamethasone sodium phosphate, 0.2kg of sodium bisulfite, 0.01kg of EDTA-2Na, 10kg of dimethyl acetamide and the balance of water for injection. The injection liquid reasonably mixes usage of all the components by a plurality of types of drugs, has fast effect for treating pant disease of the pig, addresses both the symptoms and root causes, reduces drug resistance of pathogenic microorganism and leads the pig to accelerate restoring. Simultaneously, the compound kanamycin sulfate injection liquid has convenient use, and can achieve the treatment effect of a plurality of types of drugs.

The invention relates to a drug for curing intestinal infection of livestock. The drug for curing the intestinal infection of the livestock comprises 10-15 parts of kanamycin sulfate, 12-18 parts of water-soluble bacillus subtilis, 3-7 parts of berberine hydrochloride, and 65-72 parts of auxiliary materials. The drug for curing the intestinal infection of the livestock can be used for curing the livestock by being mixed in drinking water, or being mixed in feed, or being infused in the livestock, and is free of stimulation to an organism, capable of fast relieving symptoms and curing diseases, free of harmful effect in long-time usage, low in drug tolerance, and good in curing effect.

The invention relates to compound lincomycin hydrochloride injection and a preparation method thereof. The preparation method comprises the following steps: (1) adding aminopyrine and prednisolone acetate into 95% ethanol and stirring for 10-25 min until the solution is clear; (2) fetching 300 L of injection water, adding kanamycin sulfate and lincomycin hydrochloride in turn and stirring for dissolution; and (3) mixing the solutions obtained in steps (1) and (2), adding injection water until the solution volume is 1,000 L, uniformly stirring and maintaining for 15-20 min until the liquid medicine is clear to obtain the compound lincomycin hydrochloride injection. The invention has the advantages that the compound preparation of kanamycin sulfate and lincomycin hydrochloride, the aminopyrine with adjuvant therapy effect and prednisolone acetate with adjuvant therapy effect are adopted and combined for use, thus achieving antibacterial action on gram-negative bacteria and gram-positive bacteria due to the joint application of the four medicines; and the antimicrobial spectrum is wide, the antimicrobial range is expanded, pig paratyphus and certain complications can be comprehensively treated, the curative effect is good and quick, the drug resistance rate of bacteria is reduced and the curative effect is enhanced.

The invention relates to a preparation process of kanamycin sulfate for injection. The preparation process of kanamycin sulfate for injection comprises the following steps of extracting kanamycin sulfate, drying the kanamycin sulfate and preparing kanamycin sulfate powder, cleaning a reagent bottle, soaking the reagent bottle in a hydrochloric acid solution with the concentration of 0.01 mol / L-0.1mol / L, conducting spraying type flushing, drying and sterilization, quantitatively filling the kanamycin sulfate powder into the cleaned reagent bottle, filling nitrogen into the bottle, plugging a pretreated a rubber plug and using an aluminum cap to perform cover stabbing treatment. The problem that the production speed of kanamycin sulfate for injection is lower due to an existing kanamycin sulfate preparation process is solved.

The invention discloses pig feed containing fruit peels and a preparation method of the pig feed. The feed is prepared from the following components in parts by weight: 50 to 60 parts of corns, 10 to 20 parts of fruit peels, 10 to 15 parts of grass, 1 to 5 parts of drugs, 1 to 4 parts of soybean powder, 0.1 to 0.5 part of table salt, and 0.1 to 0.5 part of probiotics powder, wherein the fruit peels are a mixture of watermelon peels and seed melon peels, a mixing ratio is 2:1, the drug is a mixture of kanamycin sulfate, streptomycin sulfate and Jingfang antipyretic powder, and a mixing ratio is 1: 1: 1. The pig feed containing the fruit peels and the preparation method of the pig feed are reasonable and balance in formula, rich in nutrition, simple in production method, and convenient in mass production; and moreover, by adding the watermelon peels and the seed melon peels which contain a great number of trace elements and amino acids, the production cost is decreased, and the nutritional value of the feed is increased.

The invention provides pesticide for thoroughly treating the silver leaf disease and a use method thereof. The pesticide is prepared from, 3-4% of 72% streptomycin sulfate, 4%-5% of kanamycin sulfate, 10%-12% of 4% kasugamycin, 36%-40% of 20% Jinggangmycin, 20%-25% of ethylic acid and 20%-30% of nutrient solutions. In the use process, the modes of root irrigation, trunk injection and spraying are adopted. After the pesticide is applied to trees suffering from the disease, the disease can be rapidly cured, the aim of eradicating the disease can be achieved, the trees suffering from the disease can not suffer from the disease any more, and the fruit trees can vigorously grow, flower and fruit.

The invention discloses a plant of pseudomonad and the application thereof in the biological removal of nitrogen heterocyclic compound. The pseudomonad adopts Pseudomonas sp. BC001 CGMCC NO.2223. The strain not only can utilize quinoline as a sole carbon source and a nitrogen source to perform metabolism, but also can well grow under the condition of highly concentrated pyridine and quinoline double stroma (pyridine is about 400 mg / L, quinoline is about 500 mg / L). The removal of the invention to the pyridine mainly passes through the biological absorption, the removal to the pyridine comprises two phases of biological absorption and biological degradation, and simultaneously, the additional carbon source with proper amount has the acceleration function to the pyridine degradation. Through the detection, the intermediate product of the pyridine degradation mainly adopts 2-hydroxyquinoline and 8-hydroxyl coumarin, the main end product adopts NH 4 <+>. BC001 bacteria have no resistance to penbritin, kanamycin sulfate, chloromycetin, cyclomycin and spectinomysin, and the used ecological security is ensured.

The invention aims at providing a preparation method of a medicine for treating respiratory system infection of livestock and poultry. The medicine has rapid and accurate treatment effects on respiratory system infection and complications of livestock and poultry. The medicine is prepared by the following steps: mixing and crushing 0.2 part of trimethoprim and 0.2 part of a cosolvent, and then sieving the mixture through a 120-mesh sieve or a finer sieve; and uniformly mixing the sieved product with 1 part of tylosin tartrate, 1 part of kanamycin sulfate and 0.05-0.1 part of a pain reliever by a method of progressively increasing by equal quantity, wherein the cosolvent is citric acid, succinic acid or a compound of the two, and the pain reliever is procaine hydrochloride, lidocaine hydrochloride or a compound of the two. The synergism compound tylosin tartrate for livestock injection provided by the invention contains three components, namely the tylosin tartrate, kanamycin sulfate and trimethoprim; the problem that secondary infection cannot be well controlled due to the narrow antibacterial spectrum of the tylosin tartrate when mycoplasma infection is treated can be solved; the problem that the trimethoprim is poorly soluble in water can be solved, a synergism effect of the tylosin tartrate and kanamycin sulfate can be achieved, and the medicine has an obvious treatment effect.

The invention discloses a method for detecting kanamycin sulfate by double-signal enhanced surface enhanced Raman spectroscopy, and belongs to the technical field of food safety detection. In the invention, gold nanorod with a controllable surface plasma resonance peak is used as a Raman substrate, and a cascade oxidation-reduction reaction catalyzed by metal-doped carbon quantum dots and a switching structure controlled by a nucleic acid aptamer are combined to establish a kanamycin sulfate detection system with double enhanced Raman signals, so that the sensitivity of detecting kanamycin sulfate is further improved. The method is low in detection limit, good in specificity, simple, convenient and rapid, and suitable for ultra-sensitive detection of kanamycin sulfate in food.

The invention discloses compound kanamycin sulfate injection and belongs to the technical field of veterinary drug preparation. Each 1000L of the compound kanamycin sulfate injection is prepared from the following raw materials of 30 to 110kg of flunixin meglumine, 30 to 110kg of kanamycin sulfate, 50 to 80kg of trimethyoprim, 1 to 20kg of edentate disodium, 100 to 400L of 95% ethanol, 1 to 10L of glacial acetic acid and the balance of water for injection. The compound kanamycin sulfate injection disclosed by the invention has wide antibacterial spectrum and wide antibacterial range, can comprehensively treat swine paratyphoid and certain complication, has high and quick curative effect, reduces drug fast rate of bacteria and improves the curative effect.

The invention discloses a preparing method of a novel gel immune adjuvant, relates to the technical field of freeze-drying preparation and a preparing method thereof, and aims to provide the preparingmethod of the novel gel immune adjuvant. The technical proposal comprises that provided is the preparing method of the novel gel immune adjuvant; the novel gel immune adjuvant comprises a polymyocytes raw fluid and a freeze-drying additive, and the freeze-drying additive is basically composed of dexamethasone, sodium vitamin C, amino acid and kanamycin sulfate. The preparing method of the novel gel immune adjuvant comprises the following steps: (1) preparing the polymyocytes raw fluid; (2) filtering and concentrating the polymyocytes raw fluid; (3) adding the freeze-drying additive, mixing evenly, carrying out aseptic filtration, and sub-packing into penicillin bottles; (4) putting the penicillin bottles into a freeze dryer, and balancing for 1 h at the temperature of 5 DEG C, 0 DEG C, and -6 DEG C respectively; and freezing rapidly, reducing the product temperature to -50 DEG C, and maintaining for 3 h; (5) carrying out vacuum drying; (6) heating and drying, and lasting for 3 h whenthe product temperature reaches 30 DEG C; and (7) plugging and sealing mouths.

The invention discloses a monolithic column modified by nano cuprous sulfide and a preparation method thereof. Firstly, the hybrid monolithic column skeleton was synthesized, and cuprous oxide nanocubes with a certain thickness were physically adsorbed on its surface, and more stable cuprous sulfide was obtained by treating with sodium sulfide solution, that is, a monolithic column decorated with nano-cuprous sulfide was obtained. The monolithic column obtained by this method has the characteristics of easy fabrication and high permeability of conventional monolithic columns. At the same time, the introduction of nano-cuprous sulfide provides specific adsorption sites, which have strong adsorption for carboxyl, amino and nitrogen heterocyclic structures. Moreover, the specific surface area of ​​the material is significantly increased. The material was prepared into a capillary solid-phase extraction column to realize high-capacity enrichment of kanamycin sulfate in milk under alkaline conditions.

The invention relates to a compound synergistic tylosin tartrate. The invention aims to provide an compound synergistic tylosin tartrate for injection for livestock, which has quick and definite curative effect on livestock and fowl respiratory system infections and complications. The medicine is composed of the following components in parts by weight: 1 part of tylosin tartrate, 1 part of kanamycin sulfate, 0.2 part of trimethoprim, 0.2 part of cosolvent and 0.05-0.1 part of pain relieving agent. The cosolvent is citric acid or succinic acid or compound of both, and the pain relieving agent is procaine hydrochloride or lidocaine hydrochloride or compound of both. The compound synergistic tylosin tartrate for injection for livestock contains the three pharmaceutical components, including tylosin tartrate, kanamycin sulfate and trimethoprim. The invention solves the problem of incapability of well controlling the secondary infection due to the narrow antimicrobial spectrum of the tylosin tartrate in treating mycoplasma infection, solves the problem of insolubility of trimethoprim in water, has a synergistic effect on tylosin tartrate and kanamycin sulfate, and is accurate in dosage and convenient to use.

The invention relates to a compound synergistic tartaric acid tylosin for injection of livestock and aims at providing a compound synergistic tartaric acid tylosin, which can be used for achieving a rapid and definite curative effect on the breathing system infection and complications of livestock, for injection of livestock. The medicine comprises the following components in parts by weight: 1 part of tartaric acid tylosin, 1 part of kanamycin sulfate, 0.2 parts of trimethoprim, 0.2 parts of cosolvent and 0.05-0.1 parts of pain alleviator, wherein the cosolvent is citric acid or succinic acid or a combination of the citric acid and the succinic acid; and the pain alleviator is procaine hydrochloride or lidocaine hydrochloride or a combination of procaine hydrochloride and the lidocaine hydrochloride. The compound synergistic tartaric acid tylosin for injection of livestock contains medicine components of three types, namely tartaric acid tylosin, kanamycin sulfate and trimethoprim, can be used for solving the problems that tartaric acid tylosin is narrow in antimicrobial spectrum and cannot well control secondary infection during the treatment of mycoplasma infection and the problem that trimethoprim is insoluble in water, and achieving a synergy effect on tartaric acid tylosin and kanamycin sulfate, and is accurate in dosage and convenient in use.

The invention relates to a culture medium for isolating and counting Clostridium butyricum, belonging to the field of microorganism separation and counting. Including tryptone 15.0g, beef extract powder 3.0g, yeast extract powder 1.0g, sodium chloride 5.0g, L-cysteine ​​hydrochloride 0.5g, sodium thioglycolate 0.5g, xylose 10.0g, Bromothymol blue 0.04g, agar powder 15.0g, purified water 900ml, D-cycloserine 0.25g, kanamycin sulfate 0.012g, egg yolk 25ml. Compared with the prior art, the beneficial effects of the present invention are: first, the culture medium of the present invention can make Clostridium butyricum It can be clearly distinguished from other bacteria by visual observation, thereby achieving the purpose of isolating and counting Clostridium butyricum. Second, the effect is remarkable, direct and effective, and low in cost.

The invention discloses a method for simultaneously detecting the emergence rate and insect resistance purity of transgenic Bt insect-resistant cottonseeds. The method is characterized by comprising the following steps of: (1) preparing a kanamycin sulfate aqueous solution with concentration between 0.4 and 0.6 percent; (2) preparing wet sand; (3) sowing; and (4) culturing. Under a selective pressure applicable to antibiotics, the method adopts a mechanism that a product which is encoded with a neomycin phosphotransferase (npt II) gene carried by a transgenic Bt plant as a label can inactivate kanamycin sulfate and other aminoglycoside antibiotics to grow normally, but a non-transgenic Bt plant does not contain an npt II gene serving as a label and cannot grow normally, so that the insect resistance purity can be detected while the emergence rate of the transgenic Bt insect-resistant cottonseeds is detected. The method is easy and practical and is low in cost, and the detection accuracy of the method can be 100 percent.