35 results about "Bacillus g" patented technology

The invention relates to a phosphorus and potassium decomposing biological organic fertilizer prepared from bacillus megatherium and Bacillus mucilaginosus. The fertilizer comprises the following components in percentage by weight: 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of humic acid, 15-20% of crop straw, 1-2% of microelements and 4-6% of culture. A preparation method of the fertilizer comprises the following steps of: 1, inoculating bacillus megatherium to a nutritional gravy culture medium and the bacillus mucilaginosus to a silicate bacteria culture medium, and spraying a seed liquid after primary and secondary cultures onto a carrier to prepare conidial powder; 2, uniformly mixing 30-40% of animal dung, 20-30% of kitchen waste, 15-25% of turf, 15-20% of straw powder, 1% of potassium dihydrogen phosphate, 1% of zeolite powder, 1% of lime, 3-5% of boric sludge and 1% of gypsum; adjusting water content to 50-60% and stacking the materials into a fermentation tank; adding enzyme microorganisms and turning over mechanically; and fermenting for 3-4 weeks and drying, and crushing to obtain a finished product and packaging. According to the biological organic fertilizer, not only is the soil capacity enhanced, but also nutritional elements which can be absorbed and utilized are provided for crops.

The invention discloses a microbial deodorant as well as a preparation method and application thereof. The microbial deodorant comprises bacillus and filamentous fungus. The preparation method comprises the steps of: uniformly mixing bacillus fermentation liquor and filamentous fungus fermentation liquor, and preparing the mixed liquor into lyophilized powder according to the conventional method in the field; or only uniformly mixing microbial fermentation liquor and excipient liquor; or only uniformly mixing the bacillus fermentation liquor and the filamentous fungus fermentation liquor. The microbial deodorant is simple to prepare and can be used for quickly removing peculiar smells and simultaneously removing smelly-source substances completely.

The invention relates to the field of soil remediation and discloses an in-situ bioremediation preparation for heavy metal contaminated soil. The preparation contains fungi and / or bacteria with heavy metal degrading abilities, the fungi refer to at least one of aspergillus niger, rhizomucor variabilis and grey cunninghamella, and the bacteria refer to at least one of bacillus amyloliquefaciens, bacillus licheniformis and bacillus simplex. By using the preparation for in-situ bioremediation of the heavy metal contaminated soil, the problems that the soil needs to move by using a moving method and secondary chemical agent pollution of the cultivated land is easily caused by using a decomposition method can be overcome, at the same time, residual heavy metals in the soil obtained after catabolism through the fungi and / or bacteria can also be fully used, the problem that high residual heavy metals in the soil seriously endanger food safety is solved, the soil micro-ecology is improved, the cultivated ability of soil is more quickly recovered, and the method is relatively low in cost and has broad application prospects.

The invention discloses a composite soil repair agent comprising microorganisms as well as a preparation method and application thereof. The composite soil repair agent comprises a porous carrier anda composite microbial agent loaded in the porous carrier, wherein the porous carrier comprises charcoal, calcium carbonate and diatomite, and the composite microbial agent comprises bacillus subtilis,iron-reducing bacteria, lactobacillus, aspergillus oryzae and vulcanized bacteria. The preparation method comprises the following steps: S1, mixing fermentation solutions of various microorganisms contained in the composite microbial agent to obtain a composite microorganism bacterial solution; S2, mixing the diatomite, calcium carbonate and charcoal in water, adding the composite microorganism bacterial solution, and uniformly mixing to obtain a dispersion solution; and S3, dropwise adding the dispersion solution into a magnesium sulfate solution, generating spherical particle precipitates,centrifugally separating the spherical particle precipitates, dropwise adding an acid solution into the separated spherical particle precipitates until no bubble is generated, washing with water to obtain a solid substance, and centrifugally drying to obtain the composite soil repair agent.

The present invention utilizes the crop waste material straw and stalk as raw material and adopts the bacillus, photosynthetic bacterium, microzyme, Aspergillus oryzae and seed liquor of basidiomycetes as beneficial microbial pool, and makes inoculation at room temp. to make the straw and stalk undergo the process of solid fermentation so as to obtain microbial fermented feed.

The invention belongs to the technical field of soil improvement and particularly relates to a preparation method and application of a greening plant waste organic medium capable of realizing soil remediation. The preparation method comprises the following steps: performing crude crushing and fine crushing on greening plant wastes to form powder with the length of 0.5-1.5cm; then putting the powdery greening plant wastes in a closed greenhouse for fermentation, controlling the carbon-nitrogen ratio of the wastes to (25:1)-(35:1), and then blending fermentation water containing bacillus, trichoderma and EM (effective microorganism) bacteria for mixing; controlling a fermentation mixture in a closed environment at the temperature of 50-65 DEG C for fermentation; and fermenting the greening plant wastes with the water content of 40-55% in fermentation process for 14-20 days to form the fermentation organic medium. The organic medium and raw soil are mixed according to a certain proportion. The mixed soil has the advantages of light weight, good bulkiness, porosity and water permeability, high content of organic matters and rich nutrients and is suitable for application in urban greening soil improvement and exhibition greenhouse and roof greening.

The invention discloses a preparation method of a composite biological deodorant. The method is characterized in culturing a composite microbe flora. The composite microbe flora mainly comprises beneficial floras of bacillus, photosynthetic bacteria, lactic acid bacteria, bifidobacteria, yeasts, actinomycetes, and the like. Various metabolites produced during the growth of the flora assist in effectively removing foul odor in environment, and assist in improving the environment. Therefore, manure can be turned harmless and can be turned into a resource. The preparation method of the composite biological deodorant is that a facultative anaerobic culture method is combined with a special process, and a composite biological product is produced. The method is advantaged in simple fermentation process, no requirement on complicated equipment, high production efficiency, and low production cost. The method has good economic and social benefits. The method is suitable to be popularized in manure treatment plants, garbage stations, farms, and industrial and agricultural production and sewage discharging sites.

A new class of imidazolines as 4-position acids or esters with very potent anti-inflammatory as well as antimicrobial activity is described. The synthesis of these imidazolines includes a multicomponent reaction applicable to a combinatorial synthetic approach. The combination of these two key characteristics provides an effective therapeutic drug in the treatment of septic shock as well as many other inflammatory (arthritis and asthma) and infectious disorders. The use of this novel class of non-steroidal agents as anti-inflammatory agents (for the treatment of asthma etc.), antibacterial agents and antiseptic agents is described. The compounds are also useful in the treatment of tumors (such as cancers). The imidazolines are potent inhibitors of the transcription factor NF-κB as well as potent activity against the Gram (+) bacterium B. subtillus and B. cereus with MIC values in the range of 50 μm / mL.

The invention provides a skin whitening and brightening facial mask and a preparation method of the skin whitening and brightening facial mask, and belongs to the field of cosmetics for daily use. Theskin whitening and brightening facial mask comprises arbutin, mannan, glycosylglycerol, jojoba ester, a citrus peel extract, glycerinum, hyaluronic acid, bacillus / glutamic acid fermentation product filtrate, water, and oligopeptide-1. The preparation method comprises the following steps: dissolving the arbutin, themannan, the glycosylglycerol, the jojoba ester and the citrus peel extract in waterto form a first mixture; mixing the glycerinum, the hyaluronic acid and the bacillus / glutamic acid fermentation product filtrate with water to form a second mixture; mixing the first mixture and thesecond mixture, and then mixing with an aqueous solution of the oligopeptide-1. The skin whitening and brightening facial mask adopts the plant extracts and the microbial fermentation products as theactive ingredients, the stability of the product is kept without adding preservatives, and the skin whitening and brightening facial mask is green, environmentally friendly and non-irritant.

The invention relates to a method for degrading congealable and highly-viscous crude oil by using microbes and feeding the crude oil through a pipeline, which comprises following steps: (1) mixing G3 solution of Pseudomonas, B5 solution of Bacillus and Y2 solution of Corynebacterium with a weight ratio of (1-6):(2-5):(2-4), and stirring completely to obtain a compound bacterial solution with a pH value within 4.0 to 8.0 and a concentration of 10<6> to 10<9> counts / ml; (2) preparing a nutrient solution from honey 0.1 to 5%, (NH4)2SO4 0.1 to 1%, MgSO4*7H2O 0.001 to 0.2%, K2HPO4 0.001 to 5%, and water allowance by mixing uniformly; (3) mixing the compound bacterial solution with the nutrient solution at a ratio of 1:(2-40) to obtain a mixture solution; (4) mixing the mixture solution with the crude oil at a ratio of (1-5):1, reacting with the microbes at 35 to 70 DEG C for 70 to 200 h while stirring with a stirring speed of 1-600 rpm, dehydrating to a water content of 1 to 10%, and feeding the crude oil in the pipeline at a temperature higher than the congealing point of the crude oil by 1 to 10 DEG C; and (5) pumping a portion of the crude oil back to a tank through the pipeline, the volume ratio of the pumped crude oil to the crude oil in the tank being 1:(4-20). The method is simple and remarkable.

The invention discloses a method for removing free gossypol from cotton dregs by a microbial fermentation method, which comprises the following steps of: a, preparing cotton dreg raw materials; b, adding feed water into the raw materials, wherein the weight ratio of the cotton dreg raw materials to water is (1:0.3)-0.8; c, inoculating microbial strains according to a certain ratio, wherein the microbial strains comprise 0.1 to 10 percent of bacillus licheniformis, 0.1 to 10 percent of lactobacillus casei and 0.1 to 10 percent of saccharomyces cerevisiae according to the percentage ratio of the weight or volume of a strain-containing culture medium to the weight of the cotton dreg raw materials; d, controlling temperature and fermenting at the temperature of between 30 and 50 DEG C; and e,after the step d is finished, drying, crushing, inspecting and packaging the fermented cotton dregs. In products obtained by the method directly, the free gossypol in the cotton dregs can be degradedeffectively, and the protein quality of the cotton dregs can be improved, so that the utilization rate of the cotton dregs in various livestock and poultry feeds is improved effectively.

The invention discloses a method for producing livestock feed ingredients using cellulase treatment of tobacco wastes. At first, tobacco stems are smashed; then cellulase solutions are sprayed, and the mass of the cellulase solutions is 5%-25% of the mass of tobacco stem powder; then bacillus bacteria liquid with a nornicotine function is sprayed, and the mass of the bacillus bacteria liquid is 5%-10% of the mass of the tobacoo stem powder; or the cellulase solutions and the bacillus bacteria liquid are sprayed simultaneously; after the cellulase solutions and the bacillus bacteria liquid are mixed uniformly and stacked in a shade and well drained corner for 3-5 days, the feed ingredients are obtained; then the feed ingredients are added to livestock feed at a ratio range of 10%-30% of the livestock feed mass to feed livestock. The method for producing the livestock feed ingredients using the cellulase treatment of the tobacco wastes makes full use of the tobacco wastes, uses the cellulase to degrade the cellulose part in the tobacco stems to cellobiose and glucose and uses nornicotined microorganisms to degrade the nicotine in tobacco leaves, the obtained feed ingredients are added to the normal feed at the ratio range of 10%-30% of the livestock feed mass to feed the livestock, the livestock weight of a control group increases by an average of 5%-10% of the livestock weight of a blank group, and the economic cost is saved by 8%-25%.

The invention discloses a method for industrially and ecologically breeding litopenaeus vannamei shrimps in indoor space. The method includes an industrially and ecologically indoor breeding process and a management method of the litopenaeus vannamei shrimps. According to the method, a traditional shrimp breeding technique is innovated, healthiest young litopenaeus vannamei is selected, the shrimps are stocked by the aid of the most reasonable density and fed by the aid of diatoms such as micro-thalassiosira rotula and chaetoceros mulleri and high-quality natural organism baits such as fairy shrimp nauplii, part of artificial feed and baits are replaced, nutrition can be enhanced, immunity can be improved, water quality change of a shrimp pond can be effectively controlled, self-cleaning capacity of water is enhanced, growth of harmful bacteria is restrained by the aid of beneficial bacteria such as lactic acid bacteria, bacillus and pseudomonades, residual feed, shrimp remains and thelike are decomposed, and a stable ecological system of the shrimp breeding pond is built. According to the method, healthy, rapidly-grown, high-stress-resistant and high-quality shrimps can be bred,shrimp breeding survive rate can be increased, shrimp breeding cost is saved, and the shrimp quantity requirements of clients are met, so that economic benefits are improved.

The invention discloses a method for producing biological feed through aerobic anaerobic coupling fermentation. According to the method, mixed bacteria of bacilli, lactobacilli and saccharomycetes serve as a fermentation strain of the biological feed; the biological feed is produced by using the aerobic anaerobic coupling solid state fermentation technology, and sterile constant-temperature air isintroduced into a deep material intermittently in the fermentation process, so that growth and propagation of aerobic microorganisms in a lower layer material are facilitated, and the product qualitystability is also improved; the stacking height of material layers can be increased under the ventilation and heat dissipation conditions, and the production capacity of equipment is improved. In addition, the fermented biological feed contains a lot of probiotic bacteria capable of improving intestinal health of animals, the micro-ecological environment of the gastrointestinal tracts can be improved after the animals are fed, and the immunity of organisms can be improved. Besides, the biological feed is rich in lactic acid and has strong mellow fragrance so that the palatability of the feedcan be remarkably improved, the animal feed intake can be improved, the rapid growth of the animals can be promoted, and the production capacity of the breeding industry can be improved.

The invention relates to a preparation method of a composite biological deodorant. The composite biological deodorant comprises a deodorant component and an extract product component according to a mass ratio of 1:1, wherein the deodorant component comprises, by mass, 20-40% of Lactobacillus plantarum, 20-40% of beer yeast, 1-5% of Rhodospirillum sp. and 1-5% of Bacillus subtilis; the extract product component comprises, by mass, 1-3% of lactic acid, 0.5-1% of citric acid, 0.02-0.05% of sodium benzoate, 1.5-2% of a natural plant extract product and 90-95% of water; and the natural plant extract product comprises, by mass, 10-20% of a Radix Sanguisorbae extract product, 20-30% of a mint extract product, 15-25% of a Lagerstroemia indica extract product and 15-20% of a sweet-scented osmanthus extract product. The composite biological deodorant has the advantages of low cost, high efficiency, wide adaptation and high practicality in concrete production.

The invention relates to a drug for curing intestinal infection of livestock. The drug for curing the intestinal infection of the livestock comprises 10-15 parts of kanamycin sulfate, 12-18 parts of water-soluble bacillus subtilis, 3-7 parts of berberine hydrochloride, and 65-72 parts of auxiliary materials. The drug for curing the intestinal infection of the livestock can be used for curing the livestock by being mixed in drinking water, or being mixed in feed, or being infused in the livestock, and is free of stimulation to an organism, capable of fast relieving symptoms and curing diseases, free of harmful effect in long-time usage, low in drug tolerance, and good in curing effect.

This invention provides a process of sterilizing a medical device, and preferably the contents of a sealed container which comprises said medical device, comprising the step of exposing said medical device to monochromatic ultraviolet radiation whereby the Dvalue of Bacillus stearothermophilus (ATCC 7953) is at least 23.7 mJ / cm2 monochromatic ultraviolet radiation at 257 nm to the spore. Further, this invention provides a process of sterilizing a medical device comprising the step of subjecting said medical device to monochromatic ultraviolet radiation wherein the minimum total energy density of said monochromatic ultraviolet radiation at 257 nm which reaches the microorganisms present on said medical device is at least 284 mJ / cm2.This invention further provides an apparatus for delivering UV radiation to a medical device for sterilization comprising a laser and a scanner for the laser such that at least 284 mJ / cm2 at 257 nm is applied to a treatment area for said medical device. This invention provides a process and apparatus in which sterilization can be achieved in less than 20 seconds, preferably less than 15 seconds, more preferably in less than 5 seconds. The process and apparatus are efficient and continuous.

The invention provides a spent bleaching earth and desulfurized gypsum based modifying agent and a modification method of saline and alkaline land. A raw material formula of the modifying agent is prepared from 65 to 75 percent of spent bleaching earth, 0.5 to 2 percent of compound microbial inoculant, 3 to 6 percent of phytic acid, 10 to 15 percent of desulfurized gypsum and 3 to 21.5 percent of fatty alcohol-polyoxyethylene ether, wherein the compound microbial inoculant is a mixture prepared from bacillus subtilis, bacillus licheniformis, bacillus laterosporus, streptomyces jingyangensis and bacillus mucilaginosus according to the volume ratio of a culture solution, which is 1 to (1.2 to 1.6) to (0.5 to 0.7) to (1.2 to 1.5) to (0.6 to 0.9). By adopting the modifying agent provided by the invention and combining the modification method provided by the invention, the modification of the saline and alkaline land, particularly heavy metal polluted saline and alkaline land, can be effectively realized; the control requirements of the saline and alkaline land are met fundamentally; meanwhile, the spent bleaching earth and desulfurized gypsum based modifying agent is low in cost; the resource reutilization can be realized; the environmental pollution is decreased.

The invention discloses a plant extracted eye essence liquid. The plant extracted eye essence liquid comprises, by weight, 40 to 60 parts of water, 1 to 10 parts of butylene glycol, 1 to 10 parts of glycerin, 1 to 15 parts of honey extracted product, 1 to 8 parts of herba portulacae extract product, 1 to 8 parts of beta-glucan, 1 to 8 parts of a bacillus fermentation product, 1 to 10 parts of 1, 2-hexalene glycol, 0.1 to 2 parts of hydroxyacetophenone, 0.1 to 3 parts of gelatin, 0.1 to 0.5 part of allantoin, 0.1 to 0.8 part of 1, 2-pentanediol, and 0.1 to 1 part of sodium hyaluronate. The plant extracted eye essence liquid is capable of supplying skin with water, relieving eye fatigue, increasing skin elasticity, preventing generation of dark eye circles and under-eye puffiness, and the toxic and side effects of chemical synthetic additives are avoided.

The present compositions and methods relate to a beta-mannanase from Paenibacillus kribbensis, polynucleotides encoding the beta-mannanase, and methods of make and / or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and / or glucomannan (GM).

The invention discloses a method special for restoring hardened soil and belongs to the technical field of soil environment restoration. The method includes: using a ploughing machine and a rotary tiller for ploughing and rotary tilling of hardened soil, and applying bio-fertilizer capable of improving soil hardening and increasing looseness and porosity into the soil after rotary tilling, whereinthe bio-fertilizer is composed of basic fertilizer and target microbial flora including garden dry branch and fallen leaf decomposed fertilizer and microorganisms like Jingyang streptomycetes, photosynthetic bacteria, aspergillus oryzae, bacillus subtilis, bacillus licheniformis and bacillus megaterium, and each gram of the bio-fertilizer contains about 0.52-1.8 billion of the above bacteria. Themethod is simple and easy to implement, convenient for operation, low in restoring cost, capable of obviously improving soil hardening symptoms, substantially enhancing buffering ability of the soil,improving breathability and water permeability of the soil, promoting water and moisture retaining of the soil and enhancing drought resistance, cold resistance and flooding resistance of plants; living microorganisms in the bio-fertilizer can strengthen leaf protection films, resist pathogenic bacteria infection and improve disease resistance and insect resistance of the plants, so that the method is obvious in restoring effect and lasting in time effect.

The invention provides a saline-alkali soil improver based on spent bleaching earth and waste residues and a saline-alkali soil improvement method. The raw material formula of the improver comprises the spent bleaching earth, a compound microorganism bacterium agent, humic acid, waste residues and a binder, wherein the compound microorganism bacterium agent is a mixture composed of bacillus subtilis, bacillus licheniformis, bacillus laterosporus, streptomyces jingyangensis and bacillus mucilaginosus, the nutrient solution volume ratio of which is 1 to (1.2-1.6) to (0.5-0.7) to (1.2-1.5) to (0.6-0.9); the mass percentages of the compound microorganism bacterium agent and the spent bleaching earth in the raw material formula are respectively 0.5%-2% and 45%-80%; the waste residues are acetic acid residues and / or starch pulp. The improver and improvement method have the beneficial effects that the improvement of saline-alkali soil can be effectively realized by adopting the improver in combination with the improvement method; the improvement requirement of the saline-alkali soil is fundamentally satisfied; meanwhile, the cost of the improver is low; the resource reutilization can be realized; the environmental pollution is reduced.

The invention relates to the technical field of pharmacy and provides a wormwood catalytic aging technology. The wormwood catalytic aging technology comprises carrying out air drying on fresh wormwoodat 28-33 DEG C at humidity of less than 20%, carrying out surface sterilization on the air-dried wormwood, spraying a microbial agent containing 6*10<9> to 12*10<9> per milliliter of bacilli to the wormwood subjected to surface sterilization, carrying out airing, putting the wormwood into a closed container, and feeding ethylene oxide and oxygen into the closed container so that pressure in the closed container is greater than or equal to the external pressure, an ethylene oxide concentration in the closed container is 250-500 g / m<3> and an oxygen concentration is 730-1260 g / m<3>. The wormwood catalytic aging technology effectively shortens wormwood aging time. The invention provides wormwood prepared through the wormwood catalytic aging technology. The wormwood has a short aging period and has properties similar to or better than those of the natural aged wormwood.

The invention relates to an active constituent extracted from a plant of altingia yunnanensis and application thereof, belonging to the technical field of biological medicine. The active constituent extracted from the plant of the altingia yunnanensis is obtained through the following steps of: a. washing and cleaning branches or leaves of the altingia yunnanensis with water and baking the cleaned branches or leaves in a baking oven for 12 hours at 60 DEG C; b. cutting the baked branches or leaves of the altingia yunnanensis into pieces, putting in a triangular flask, soaking the cut branches or leaves with 100 percent methanol and extracting the cut branches or leaves on a water bath kettle at 40 DEG C, wherein the soaking extraction of the first time lasts for 2 hours, and the soaking extraction of the second time lasts for 4 hours and the soaking extraction of the third time lasts for 8 hours; c. merging extracting liquid obtained by extracting three times and concentrating the extracting liquid at 60 DEG C to obtain extractum; and d. dissolving the extractum with methanol, metering the volume to be 10mg / ml and placing in a refrigerator at 4 DEG C, and storing for later use. The extracted active constituent has stronger inhibiting effect on human pathogenic bacteria of bacillus cereus, can be used for preparing preparations for inhibiting the human pathogenic bacteria of the bacillus cereus and can be developed into novel medical antibiotics.

Disclosed is a high-yield maize culture method. The method comprises the steps of seed treatment, field planting, field management and water and fertilizer management. The high-yield maize culture method is characterized in that according to seed treatment, maize seeds are treated through an organic seed coating for maize, and the seed coating is prepared from actinomycin, bacillus mucilaginosus, azospirillum brasilense, kieselguhr, carry powder, heteroauxin, potassium sulphate, magnesium sulfate, zinc sulfate, alpha-pimacol growth regulator, EDTA-2Na chelating agent, humic acid and grass peat.

The invention discloses a vegetable planting method, which includes ploughing and fertilizing, seed selection and seedling cultivation, seedling transplanting and insect control. A seedling cultivation medium is prepared from bacilli, nitrogen-fixing bacteria, pseudomonas and bacillus licheniformis in a mass ratio of 1:1:1:1. A process includes steps: well mixing the bacteria to obtain mixed bacteria powder, adding into a container, and adding maltose under the condition that a mass ratio of maltose to the mixed bacteria powder is 10:1; adding water into the container, stirring into paste, andquickly cooling and drying at a low temperature to obtain a bio-fertilizer. By the nitrogen-fixing bacteria, the content of nitrogen in soil can be increased; by application of bacilli and pseudomonas, indissolvable phosphorus and potassium in soil can be separated out and converted into phosphorus and potassium compounds which can be absorbed and used by plants, and accordingly nutrient elementsupply in a plant growth environment is increased, organics in soil are increased, and soil fertility is improved.

The invention discloses a disinfectant used for a radiation room and a preparation method thereof and belongs to the technical field of medical disinfection. The disinfectant disclosed by the invention is prepared from the following raw materials: povidone iodine, fucoidan sulfate, peroxyacetic acid, cichorium endivia, aristolochia, lavender, cactus, aloe, cassia tora, astragalus, oriental wormwood, fritillaria cirrhosa, chrysanthemum, selenium-enriched tea, liquorice and silver nitrate. The disinfection method disclosed by the invention has the advantages that detection rate on bacteria such as bacillus licheniformis is reduced year by year, CT room infection rate is 10.9-12.1%, blood vessel-related infection rate is 3.1-3.9%, respiratory tract infection rate is 9.4-10.9%, viral infection rate is 0.2-0.4%, skin and mucous injury severity is 1.07-1.19%, infection can not be caused, and cost is low.