Microbial deodorant as well as preparation method and application thereof
A deodorant and microorganism technology, applied in the field of microbial deodorant and its preparation, can solve the problems of insufficient and incomplete chemical deodorization effect, odor problem, unsatisfactory deodorization efficiency, secondary pollution, etc. Maintains deodorizing effect, removes odor quickly, and eliminates odor
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Embodiment 1
[0033] Preparation of microbial deodorant:
[0034] Mix Bacillus licheniformis CGMCC 1.265, Bacillus coagulans CGMCC 1.2009 and Monascus ruber CGMCC 3.549 according to the percentage of the following bacteria in the total number of bacteria: Bacillus licheniformis 50%, Bacillus coagulans 30%, and Monascus 20%; then uniformly mix the bacterial liquid and the excipient liquid according to the volume ratio of 1:10.
[0035] Wherein, the formula of excipient liquid is glycerol 10%, water 80%, carbohydrate glucose 5%, trace element 4%, spice 1%.
[0036] Among them, the slant medium, primary seed medium and fermentation culture used by Bacillus are nutrient broth medium, the specific formula: 0.3% peptone, 2% beef extract, 1% sodium chloride, pH 7.0; , the slant medium also contains 2% agar powder, wherein, the cultivation temperature of the primary seed cultivation is 37°C, the cultivation time of the primary seed is 2 days, the cultivation temperature of the fermentation cultiva...
Embodiment 2
[0039] Preparation of microbial deodorant:
[0040] Mix Bacillus licheniformis CGMCC 1.1858, Bacillus coagulans CGMCC 1.949 and Monascus ruber CGMCC 3.4636 according to the percentage of the following bacteria in the total number of bacteria: Bacillus licheniformis 30%, Bacillus coagulans 30%, Monascus 40%; then the bacterial liquid and the excipient liquid are uniformly mixed according to the volume ratio of 1:50.
[0041] Wherein, the formula of the vehicle is 10% of glycerol, 80% of water, 5% of carbohydrate sodium acetate, 4% of trace elements, and 1% of fragrance.
[0042] Among them, the slant medium, primary seed medium and fermentation culture used by Bacillus are nutrient broth medium, and the specific formula is: 2% peptone, 0.3% beef extract, 0.3% sodium chloride, and a pH of 7.0; , the slant medium also contains 1.4% agar powder, wherein, the cultivation temperature of the primary seed cultivation is 30°C, the cultivation time of the primary seed is 1 day, the cul...
Embodiment 3
[0045] Preparation of microbial deodorant:
[0046] Mix Bacillus licheniformis CGMCC 1.518, Bacillus coagulans ATCC 10545 and Monascus ruber CGMCC 3.4701 according to the percentage of the following bacteria in the total number of bacteria: Bacillus licheniformis 30%, Bacillus coagulans 30%, Monascus 40%; When in use, the mixed bacterial solution can be directly diluted 100 times with water.
[0047] Among them, the slant medium, primary seed medium and fermentation culture used by Bacillus are all nutrient broth medium, and the specific formula is: 1% peptone, 1% beef extract, 1% sodium chloride, and a pH of 7.0; , the slant medium also contains 1.8% agar powder, wherein, the cultivation temperature of the primary seed cultivation is 34°C, the cultivation time of the primary seed is 2 days, the cultivation temperature of the fermentation cultivation is 34°C, the fermentation cultivation is 3 days, and the fermentation cultivation The inoculum size was 10%. Afterwards, the ...
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