The invention discloses a water-based nanometer titanium dioxide photocatalytic sterilization purification coating and a preparation method thereof. The coating is prepared from 38-55 wt% of nanometertitanium dioxide, 10-30 wt% of a waterborne organosilicone modified acrylic emulsion, 2-5 wt% of silicate, 1.5-3.5 wt% of an inorganic antibacterial agent, 1-3 wt% of negative ion micro-powder, 1.4-5.0 wt% of a functional assistant, 2-4 wt% of a silane coupling agent, 0.4-0.8 wt% of a wetting dispersant, and the balance of water. The coating has air purifying and bacterium killing effects, is green and environmentally friendly, has excellent anti-aging property, scrub resistance, cohesiveness, corrosion resistance and the like, can directly replace putty powder and a latex paint, and can be directly firmly bonded with a cement mortar wall without peeling, shedding or cracking.