Production method of straw and stalk microbial fermented feed
A technology of microbial fermentation and production method, which is applied in the field of straw microbial fermentation feed production, can solve the problems of high cost, large number of bacterial species, complicated operation, etc., and achieve the effects of low cost, good product quality and simple operation.
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[0019] 1. Bacillus (producing protease, amylase, cellulase, peptide antibiotics, etc.) flora liquid seed fermentation steps are as follows:
[0020] 1) Activation of seed mothers: move Bacillus pumilus (for example: Bacillus pumilus, Bacillus subtilis, Bacillus sp., Paenibacillus amyloliguefaciens) seed mothers to the slopes of the newly prepared PDA medium respectively, and activate and cultivate for 24--48 hours , the temperature is 28°C, and the thalline in the activated and mature Kelvin flask is called a seed;
[0021] Seed parent activation culture adopts potato-sucrose agar medium (abbreviated as PDA medium) to form by weight percentage: 1000mL of 20% potato (potato) extract; 20g of sucrose, 18g of agar, pH7.0~7.2;
[0022] 2) Seed tank inoculation and fermentation culture: wash 1 bottle of Bacillus seeds in the activated Kjeldahl flask with 200mL sterile water into a 500mL sterile Erlenmeyer flask, and inoculate to 50mL by flame inoculation method or differential press...
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