The invention provides a production method of a single-
cell protein. The production method comprises the steps of (1) evenly mixing the oil extraction residues of microorganisms with
lysozyme in a
mass ratio of (20-200): 1, reacting for 2 to 5 hours under conditions of 40 to 60 DEG C, an initial pH 5.0 to 7.0 and a stirring rotating speed 50 to 200 r / minute, and treating at 105 DEG C for 10 to 30 minutes; (2) mixing water with the obtained enzymatic
hydrolysate in a
mass ratio of (5-2): 1, and adding 5-150mg / L of
magnesium sulfate, 100-200mg / L of
ferric chloride and 5-50mg / L of odium dihydrogen
phosphate, and sterilizing at 115 to 121 DEG C for 15 to 30 minutes; (3) inoculating the culture solution obtained in the step (2) with spiral seaweed and a
yeast under the conditions of a pH
ranging 6.0 to 8.0 and a volume of
aeration in the range of 0.1vvm to 5.0vvm, and employing a two-stage culture method based on a
temperature control strategy, and setting the culture time of the front stage of culture in the range of 48 to 108 hours and the culture time of the rear stage of culture in the range of 24 to 36 hours, and (4) collecting the microorganic
biomass obtained in the step (3), flushing with water, and
drying by using a freezer dryer to obtain the single-
cell protein product. The production method has the advantages that the production efficiency is improved, the production cost is reduced and the quality of the single-
cell protein is improved.