124 results about "Isoprodian" patented technology

The invention discloses a method for simultaneously extracting DNA (deoxyribonucleic acid) and RNA from lily tissue. The method comprises the following steps of: obtaining total nucleic acid solution through crude extraction and purification of the same steps from a lily tissue sample; and then sequentially selectively precipitating RNA and DNA, thereby obtaining high-quality DNA and RNA. The method disclosed by the invention is short in time, economic and fast, and good in stability; and the extracted nucleic acid is high in quality. The method has the characteristics that high-concentration potassium acetate is utilized for precipitating polysaccharides twice, so that the polysaccharides in the lily sample can be effectively removed; and in the DNA and RNA separation process, the RNA is selectively precipitated by means of the synergistic effect of lithium chloride and absolute ethyl alcohol and then the DNA is precipitated by using sodium acetate and isopropanol; and therefore, the efficiency of the DNA-RNA separation is high, the loss of the nucleic acid is low and the precipitation time is greatly shortened. The method is suitable for simultaneously extracting the DNA and the RNA from the lily tissue containing rich polysaccharides and other secondary metabolites.

The invention relates to a medicine coated balloon and a preparation method thereof. The coating is prepared from the following raw materials in parts by weight: 5-9 parts of a medicine, 1-5 parts of excipient and 1-15 parts of a solvent, wherein the medicine is at least one of taxanes and macrolides; the excipient is at least one of iopromide, polyvinylpyrrolidone, polysorbate and polyethylene glycol; and the solvent is at least one of water, methanol, ethanol, acetone, isopropanol, acetonitrile, ethyl acetate and methyl formate. According to the method, by optimizing the composition of coating raw materials, the proportion of raw materials and the process parameter in the coating preparation method, the coating cannot be easily separated before reaching the target site and can quickly release the medicine when reaching the target site, the target tissue can be promoted to absorb the medicine in the coating, the medicine duration in the target tissue can be improved, and the vessel restenosis rate after interventional therapy can be reduced.

A blood cell analyzer diluent and a hemolytic agent, are characterized in that one litre diluent is provided with 12.0-4.0g of sodium chloride, 2.0-10.0g of sodium sulfate, 0.8-0.5g of 1,3,2-methylol urea, 0.2-0.5g of copper sulfite, 3.0-8.0g of EDTA-2Na, 0.2-0.7g of Piperacillin Sodium, a borate buffering liquid toning the ph value to 7.2-7.8, and the balance is water; one litre hemolytic agent is provided with 0.8-5.0g of potassium chloride, 0-60.0g of dodecyl trimethyl ammonium chloride, 14.0-0g of octadecyl trimethyl ammonium bromide, 6.0-10.0ml of isopropanol, carbonate or alcaine buffering liquid toning the ph value to 7.2-7.8, and the balance is water. The inventive reagent can form stable hemoglobin derivatives, and the absorption spectrum curves are similar when lambada is 540nm, lambada is 504nm, which can satisfy the clinical inspection requirement; the reagent does not contain cyanide, azide, and has non-toxicity, which can effective improve working atmosphere of operating staff, and can reduce harm of toxicant to personal health.

The invention discloses chitosan ester p-aminobenzoate, which has a structure shown in the specifications, wherein X is between 0.65 and 0.95, and the molecular weight is between 70 and 80 kilodaltons. The materialization indexes of the compound are that: the chitosan ester p-aminobenzoate is a pale yellow powder solid, can be dissolved in water and is dissolved in ethanol and isopropanol slightly. After a p-animo-benzoyl structure is introduced into a chitosan molecular chain, the minimum inhibitory concentration of the compound on staphylococcus aureus and aspergillus niger is 0.1 percent and 0.25 percent respectively, so the chitosan ester p-aminobenzoate can be used as food antiseptic additives.

The invention provides an extraction method of total DNA of metagenome of fish enteric microorganisms and a kit. The extraction method comprises the following steps: (1) fish sample sampling and fixing treatment of microorganisms on the surface of the fish; (2) acquisition of enteric microorganisms and collection of thalli; (3) embedding and cracking of cells; (4) extraction of DNA; and (5) DNA preservation. The kit comprises the following main components: a solution A which is a mixed solution of Tris and EDTA; a solution B which is a mixed solution of Tris, EDTA, NaCL, PVP, guanidinium isothiocyanate, Triton-X100, PMSF, and beta-mercaptoethanol; a solution C which is a mixed solution of Tris, EDTA, NaCl and DTT; a solution D which is a mixed solution of proteinaseK, a lysozyme, Tris, NaCl and SDS; a solution E which is a mixed solution of Tris saturated phenol and chloroform; F which is isopropanol; and G which is an ethanol aqueous solution. The DNA fragment obtained by the invention is high in purity, and the content of DNA of a host and the microorganisms on the surface of the fish is small.

The invention provides a method for extracting microorganism total DNA from a coal seam water sample, belongs to coal seam water sample microorganism analysis and aims to solve the technical problem by providing the method for extracting microorganism total DNA from a coal seam water sample with complex composition. The method comprises the following steps: pretreating the coal seam water sample, centrifugally removing large particulate impurity, and collecting microorganisms in the water sample by means of membrane filtering; breaking microorganism cells, shredding a filter membrane on which microorganisms are collected, resuspending in a buffer solution, breaking cells by means of a ball mill, inoculating for 2-4 h at 55 DEG C with proteinase K and sodium dodecyl sulfate solution until a solution is clear; purifying and precipitating microorganism total DNA, removing miscellaneous proteins by phenol-chloroform process, and adding isopropanol precipitate to collect total DNA. The total DNA extracting method is suitable for coal seam water sample and related samples with complex composition and is good in cell breaking effect, and extracted genome is high in purity and good in completeness and is directly useful in subsequent metagenomics analysis, DGGE analysis and the like.

The invention provides a method for rapidly extracting DNA from whole blood. The method comprises the following steps: S1, centrifuging a blood sample at a high speed, removing a supernatant, keeping precipitate, adding a lysate, uniformly mixing till a clear state, keeping for 40-60min in a water bath with the temperature of 50-60 DEG C, cooling, performing suction extraction by using an organic solvent, centrifuging, and taking a supernatant; S2, adding isopropanol and NaAc into the supernatant, uniformly mixing, collecting the precipitate, cleaning the precipitate and airing; S3, adding a TEN buffer solution and ribonuclease into the precipitate, uniformly mixing, incubating for 30min, then adding a proteinase K containing TEN buffer solution, uniformly mixing, performing water bath for 30min, cooling, centrifuging, taking a supernatant, and purifying to obtain the pure DNA. By the method, the sample treating amount is large; most of red blood cells in the supernatant are removed through high-speed centrifugation first and then blood cells (white blood cells) in the precipitation are lysed by using the self-prepared whole cell lysate by one step so as to rapidly obtain a large number of DNAs applicable to three generations of sequencing from blood.

The invention discloses a total DNA (Deoxyribonucleic Acid) extraction method of bottom mud containing high content of humus at the river mouth. The method comprises the following steps: washing bottom mud by stroke-physiological saline solution to remove part of impurities such humus, and meanwhile, concentrating microorganisms; then, multigelating in liquid nitrogen and a water bath at 65 DEG C by means of CTAB (Cetyltrimethyl Ammonium Bromide), proteinase K and lysozyme for cell disruption, and then adding SDS (Sodium Dodecyl Sulfonate) and insulating for 2 hours in the water bath at 65 DEG C to combine protein; adding isovolumetric phenol / chloroform / isoamylol (25:24:1) to remove protein; adding isovolumetric isopropanol to set DNA, washing precipitate with 70% ethanol, and dissolving DNA by TE (pH8.0) after airing; adding 3 times of absolute ethyl alcohol in volume to re-set DNA, washing the precipitate with 70% ethanol, dissolving the precipitated DNA by TE after airing, and storing at minus 20 DEG C for later use. The method provided by the invention is simple to operate and cheap in price. The total DNA of bottom mud extracted by the method provided by the invention has the advantages of high purity and integral biodiversity, and can be used for molecular biology study and microbial diversity analysis.

A method in which DNA is extracted from a precious sample of organism simply and efficiently without being contaminated by impurities is provided. The method for extracting DNA comprises of: 1) a process for pretreatment having; a procedure for obtaining fragments by using a whole sample of organism of hard tissue, by cutting the sample, or by crushing the sample, and a subsequent procedure of decalcification by adding an EDTA aqueous solution thereto, 2) a process for dissolution having; a procedure for adding a buffer solution of a solubilizer, which dissolves a complex of DNA-protein in the sample of organism, and a proteolytic enzyme to a solution of (1) to prepare a solution of DNA, 3) a process for extraction having; a procedure for adding a saturated phenol buffer including tris(hydroxymethyl)aminomethane hydrochloride or an aqueous solution including sodium iodide and isopropanol to the solution of the complex of (2) for deproteinization, and then separating an extract of DNA, 4) a process for purification having; a subsequent process for purification of DNA by passing the extract of DNA of (3) through a column.

The invention discloses a method for extracting and separating total proteins of mice sperms by using two-dimensional electrophoresis. The method comprises the following steps: preparing sperms, precipitating DNAs of the sperms after lysis of the sperms, preparing sperm proteins, preparing a protein extraction sample, carrying out first-dimensional isoelectric focusing electrophoresis, carrying out second-dimensional SDS-polyacrylamide gel electrophoresis, dyeing by using coomassie brilliant blue, preparing gel and scanning by using an image scanner. According to the method, lysis of the sperms is completely performed by Trizol, the proteins are extracted by chloroform, impurities are removed by isopropanol, guanidine hydrochloride and ethyl alcohol, and the proteins are purified by acetone, so that the problems of low content of the mice sperms, collection difficulty, small cell volume, a small amount of cytoplasm and a large amount of acrosomal protease are well solved; the sample prepared by the method is complete to prepare, has a large quantity of protein isolating points, and is high in repeatability and clear in chromatogram; by virtue of detection of PDQuestsoftware, the number of the protein points is greater than 1,000; therefore, the method lays the foundation for further mass spectrometry.

The invention provides a total DNA (deoxyribonucleic acid) extraction method and a kit for synchronously removing humic acid and mycoprotein and relates to the field of research of metagenomics in soil microbiology and general microbiology. The method comprises the following steps: (1) suspending samples by utilizing a humic acid combination solution, so as to obtain sediments; (2) adding a buffer solution and proteinase K, and carrying out centrifuging to obtain supernate; (3) adding a coagulant to carry out coagulating sedimentation on the residual humic acid and mycoprotein, and carrying out centrifuging to obtain the supernate; (4) adding isopropanol to precipitate DNA; (5) adding 70% ethanol to wash the sediments, and blowing off the residual ethanol; (6) adding a DNA dissolving solution (TE) to dissolve the sediments, so as to obtain a sample, namely the total DNA. The kit comprises the following components: the humic acid combination solution, a PBS butter liquid, an extraction butter liquid, the proteinase K, a 20% SDS (sodium dodecyl sulfonate) water solution, the coagulant, a DNA washing agent, the 70% ethanol and the DNA dissolving solution. Due to the adoption of the method and the kit, the one-time extraction quantity of the total DNA is enough to carry out PCR (polymerase chain reaction) amplification for dozens of times.

The invention discloses a method for preparing a cell matrix-free material for a porcine adipose tissue. The method comprises the following steps: (1) taking fresh porcine subcutaneous fats, cleaning, cutting into pieces, adding water or normal saline, homogenating, and centrifuging to obtain a precipitate, and rinsing by PBS solution or water; (2) crushing the precipitate, rinsing by the SDS solution, then rinsing by the PBS solution or water, and centrifuging to obtain a precipitate; and (3) rinsing the precipitate by isopropanol solution, and then rinsing by the PBS solution or water to obtain the product. The acellular matrix material prepared by the method disclosed by the invention is thorough in decellularization, low in immunogenicity, good in biocompatibility, simple in preparation method, low in cost and good in application prospect.

The invention aims to provide a chlorhexidine gluconate composite disinfectant. The chlorhexidine gluconate composite disinfectant is prepared from chlorhexidine gluconate, coco fatty acid diethanol amide, lemon essence, delta-gluconolactone, hydroxyethyl cellulose, isopropanol, lauryl amine oxide, wool grease, tridecanol, and water for injection. The chlorhexidine gluconate composite disinfectant can perform comprehensive disinfection treatment on infection of skin, wound, trauma and burn before and after a surgery, can comprehensively disinfect the surgical instruments, has wide bacteriostatic and bactericidal effects and high effects, has a killing effect on all gram-positive bacterium, gram-negative bacterium, fungus, acid-fast bacteria, germs and viruses, is still effective in presence of serum and blood, causes no irritation to skin tissues, and is convenient and safe to use.

The invention discloses a kit for extracting blood free DNA. The kit of the invention includes a lysis solution, a binding solution, a cleaning solution 1 and a cleaning solution 2. The binding solution contains 2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HC1, 10-50mM EDTA, 5-15% Triton X-100, and 20-50% absolute ethanol or isopropanol. The cleaning solution 1 contains2-5 M guanidine isothiocyanate or guanidine hydrochloride, 10-100mM Tris-HCl, 10-50mM EDTA, 5-10% Triton X-100, SDS or Tween 20, and 20-50% absolute ethanol or isopropanol. The kit of the invention improves the binding solution and increases the extraction efficiency, improves the cleaning solution 1, increases the binding efficiency of the magnetic beads cfDNA and removes impurities, improves the recovery efficiency and purity of the cfDNA and reduces the material cost.

The invention belongs to the technical field of biological medicines, and discloses a betulinic acid derivative, a preparation method and application thereof. The preparation method comprises the following steps: preparing biotin esterification coupled oligomerization ethylene glycol carboxylic acid or biotin amide coupled oligomerization ethylene glycol carboxylic acid; dissolving betulinic acidin a solvent, and carrying out a stirring reaction at 0 DEG C for 1-6 hours under the action of a dehydrating agent and a catalyst; adding biotin or the biotin esterification coupled oligomerization ethylene glycol carboxylic acid or biotin amide coupled oligomerization ethylene glycol carboxylic acid according to a betulinic acid molar ratio of 1:1-3, heating to room temperature from an ice bath,and stirring in a dark place overnight; and concentrating the filtrate, re-crystallizing with ice diethyl ether or isopropanol, carrying out chromatography or preparative liquid phase purification, and freeze-drying to obtain the betulinic acid derivative. The betulinic acid derivative, the pharmaceutically acceptable salt and the isotope marker thereof can be applied to preparation of anti-cancer drugs and drugs for treating obesity or non-alcoholic fatty liver disease.

The invention relates to a method for determining peramivir intermediate isomers by using high performance liquid chromatography. The method is characterized in that an adopted chromatographic columnis a polysaccharide derivative coated chiral chromatographic column; the adopted mobile phase is a mixed solution of isopropanol and normal hexane, and isocratic elution is adopted in a high performance liquid chromatography system; in the mobile phase, the volume ratio of isopropanol to normal hexane is 10: 90 to 20: 80; the flow velocity of the mobile phase is 0.8 to 1.0 ml / min; an adopted detector is an ultraviolet detector, and the monitoring wavelength is 215 nm. The method overcomes the defects in the prior art, solves the problem of analytical determination of the peramivir intermediateisomer, can effectively control the contents of the target product and isomer impurities thereof, avoids the interference of the isomer impurities on the subsequent synthesis reaction, enhances the quality of the subsequent prepared peramivir, and ensures the medication safety. The invention provides an accurate and efficient detection method for determining the isomer of the peramivir intermediate.

The invention provides a method for extracting genomic DNA from swallow nests, which comprises the following steps: firstly, lysing cells in the swallow nest by use of SDS and DTT, ,and simultaneously removing most of glycosidoprotein through high-concentration NaCl, so as to reduce the interference of glycosidoprotein to DNA in the purification process; secondly, further removing the glycosidoprotein by use of combining chloroform-isoamyl aleohl with CTAB; and finally, depositing DNA by isopropanol at a low temperature in a short time, which can reduce coprecipitation of glycosidoprotein and DNA to the utmost extent. The method has the advantages of stable result, good reproduction quality, simple operation, low cost, and no need of special reagent and apparatus; moreover, the method has high quality of the obtained genomic DNA which can meet the requirement of the PCR reaction, and can realize identification of the variety of the swallow nests.

The invention relates to an RNA extraction kit suitable for normal-temperature transportation. The kit comprises a lysis binding solution, and the lysis binding solution contains guanidine isothiocyanate with the concentration of 1 to 5 M, sodium thiocyanate with the concentration of 0.2 to 3 M, trihydroxymethyl aminomethane with the concentration of 0.01 to 0.1 M, tetrasodium iminodisuccinate with the concentration of 0.001 to 0.05 M, sodium ascorbate with the mass fraction of 0.05-1%, betaine with the mass fraction of 0.1-2%, Tween 20 with the mass fraction of 1-6%, sodium acetate with the concentration of 0.05 to 0.5 M, and isopropanol withthe mass fraction of 25-55%. The lysis binding solution does not contain toxic organic solvents such as phenol and does not contain reagents such as protease K needing cold-chain transportation, so that the RNA extraction kit can be transported under the condition of normal temperature. The lysis binding solution has the effects of lysis of cells / viruses and promotion of combination of nucleic acid and magnetic beads, can complete lysis and combination in one step, and is more suitable for an automatic nucleic acid extractor. The two protein denaturants are combined for use, and compared with single use of one protein denaturant, the capacity of removing impurities such as protein with complex chemical properties can be enhanced.

A novel process for producing an optically active (E)-7-[2-cyclopropyl-4-(4-fluorophenyl quinolin-3-yl]-5-hydroxy-3-oxohept-6-enoic acid ester of formula (III) which is important as an intermediate for synthesizing medicines, comprises reacting 1,3-bis(trimethylsilyloxy)-1-alkoxybuta-1,3-diene of formula (I) with (E)-3-[2-cyclopropyl-4-(4-fluorophenyl)-quinolin-3-yl]-prop-2-en-1-al of formula (II) in the presence of an optically active binaphthol-titanium complex obtained from 1,1′-bi-2-naphthol with titanium tetraisopropoxide, a metal salt and an amine, and then subjecting the reaction product to desilylation

The invention provides a valve material with synergistic anticoagulation and anti-calcification functions and a preparation method of the valve material. The preparation method comprises the followingsteps: carrying out glutaraldehyde crosslinking treatment on an animal-derived biological valve material; immersing the treated valve material in confining liquid containing an amino compound to perform treatment for 0.5-6 h, and then confining aldehyde groups remaining after glutaraldehyde crosslinking; then putting the valve material into a reaction solution containing an anticoagulant and a cross-linking agent, and carrying out cross-linking treatment for 6-24 hours at 4-37 DEG C; and finally, cleaning the valve material to obtain the valve material, and storing the valve material in a glutaraldehyde or isopropanol / glycerol mixed solvent. The method can effectively solve the problems of easy calcification and thrombus formation caused by aldehyde group residue in the valve material prepared by an existing method, and the valve material prepared by the method disclosed by the invention can be used as a valve material required by aortic valve, pulmonary valve, venous valve, mitral valve and tricuspid valve replacement.

The invention relates to a novel coronavirus COVID-19 marker detector and a detection method. The detector comprises a laser emitting device, a light angle adjusting device, a beam expander, a gas receiving device, a signal processing device and a PC processing terminal, wherein the gas receiving device is internally provided with a triangular annular ring-down cavity, a temperature controller anda pressure gauge; laser emitted by the laser emitting device vertically enters the beam expander to be processed after passing through the light angle adjusting device, then enters the triangular annular ring-down cavity of the gas receiving device to be measured and is transmitted to the PC processing terminal through the signal processing device. According to the detection method, whether a patient with novel coronal pneumonia is diagnosed by detecting whether the concentrations of ethyl butyrate, butyraldehyde, isopropanol and acetone in respiratory gas are abnormal or not. The optical cavity ring-down spectroscopy technology has the advantages of being high in precision, short in time, low in detection cost, capable of greatly improving the medical diagnosis efficiency, good in economic space, large in research and development value and considerable in development space.

The invention discloses a preparation method of extracellular matrix-imitated printable composite biological ink, which comprises the following steps of: (A) dissolving GelMA powder and a photoinitiator in PBS, and uniformly stirring at 40-50 DEG C to prepare a GelMA solution with the final concentration of 5-15%; (B) pretreating fresh adipose tissues, mashing the pretreated adipose tissues, respectively treating the mashed adipose tissues by 0.25% trypsin-0.1% EDTA (Ethylene Diamine Tetraacetic Acid) and 3% Triton-X100 for 1 hour, and respectively treating the treated adipose tissues by 4% sodium deoxycholate and 100% isopropanol; (C) dissolving the acellular extracellular matrix obtained in the step (B) into 0.01 M HCL and 1mg / mL pepsin solution, stirring at 4-10 DEG C, and centrifugingat a low speed for 10min to obtain a deadipose extracellular matrix solution with the final concentration of 5-15mg / mL; and (D) preheating the GelMA prepared in the step (A) at a proper temperature, slowly adding the solution in the step (C) at a vortex oscillation speed of 1500-2500rpm according to a volume ratio of the GelMA to the acellular extracellular matrix of (1-4): 1, adjusting the pH value, and performing sterilizing. The composite biological ink prepared by the method has excellent printability, biocompatibility and biological activity.

The present invention relates to an one-step method ribonucleic acid extraction reagent EZRNA. Its formula includes (by 1 L total amount) guanidinium isothiocyanate 3-5 M, sodium citrate 18-25 M, sodium laurate (by wt%) 0.3-0.6%, 2-mercaptoethanol 0.6-1.2 M and balanced acidic phenol (by volume%) 40-55%. Besides, said invention also provides the concrete steps of the invented one-step method ribonucleic acid extraction process by using said reagent.

The invention belongs to the technical field of high polymer materials and relates to a polyaspartic acid type ice point regulator preparation method by taking polyaspartic acid and polyaspartic acid derivatives as the raw materials. A macromolecular polyaspartic acid type grafted copolymer is provided, and is synthesized by a midbody polysuccinimide of polyaspartic acid amino acid and a graft, wherein the graft comprises alcohol amine type substances, such as ethanol amine, isopropanolamine, diglycolamine, diethanol amine, 2-amino-1-butanol, N-(3-aminopropyl)diethanol amine, 3-amino propanol, 2-(methylamino)ethanol, D-phenylglycinol, diisopropanol amine and N-(2-ethoxy)ethylenediamine. The copolymer can be taken as an environment-friendly macromolecular ice point regulator, and has high application value in the energy saving field of a dynamic ice-slurry cold-storage air-conditioning system and the field of food additives and medical fields.

The invention relates to a phosphodiesterase 4 inhibitor apremilast composition and a quality detection method. The apremilast composition is a tablet, comprises lactose, magnesium stearate and otherauxiliary materials, and is used for treating adult patients suffering from active psoriatic arthritis, adult patients suffering from moderate-to-severe plaque psoriasis and suitable for phototherapyor systemic therapy, and adult oral ulcer patients related to Bessel's disease. The quality detection method comprises the step of determining the content of the enantiomer in the tablet, and comprises the following operations: by using a chiral chromatographic column and using n-hexane, isopropanol and the like as mobile phases, in a high performance liquid chromatography system, injecting 5 [mu]l of a system applicable solution into a liquid chromatograph, wherein the peak appearance sequence is enantiomer peaks and apremilast peaks successively, and the separation degree between two peaks is not less than 1.5, and precisely measuring 5 [mu]l of the test solution, injecting the test solution into a liquid chromatograph, recording a chromatogram, and calculating the content of the enantiomer according to an area normalization method if enantiomer peaks exist in the chromatogram of the test solution. The compositions and methods of the present invention exhibit excellent technical effects as described in the specification.

The present invention discloses high-moisture mask based on cellulose fibers. The high-moisture mask comprises modified mask base cloth and mask base liquid, the mask base liquid comprises the following raw materials by weight: 8-10 parts of sodium hyaluronate, 8-12 parts of carbomer, 6-8 parts of antimicrobial peptides, 3-4 parts of N, N-dicyclohexylcarbodiimide, 10-15 parts of triethanolamine, 8-10 parts of glycerol, 3-5 parts of collagen, 4-7 parts of sodium methylparaben and 6-8 parts of a traditional Chinese medicine additive. The modified mask base cloth is prepared by treating mask basecloth, isopropanol, sodium hydroxide, tetramethylammonium hydroxide, epoxypropane and glucose oxidase. The process design is reasonable, the component ratio is suitable, and the prepared moisturizingmask has excellent effects of supplementing water and moisturizing skin, further has excellent anti-microorganism effect, can inhibit growth of microorganisms on surfaces of the skin, can avoid occurrence of acnes, and has good practicality.

The invention discloses a high-sensitivity virus nucleic acid extraction kit, and belongs to the technical field of molecular biology. The virus nucleic acid extraction kit comprises a virus lysis solution, a magnetic bead buffer solution, a rinsing solution 1, a rinsing solution 2 and an eluent, the formula of the virus lysis solution is as follows: guanidine isothiocyanate of 1-5 M, sodium iodide of 1-5 M, SLS of 10-100 g / L, triton-100 of 10-100 mL / L, EDTA-2Na of 10-200 mM and isopropanol of 200-500 mL / L, and the pH value of the virus lysis solution is 7.0-8.0. The virus nucleic acid extraction kit disclosed by the invention has the advantages of small dosage, capability of extracting at room temperature, high sensitivity, high purity of extracted nucleic acid, wide applicability and the like.

The invention discloses a rehmannia extract, a preparation method and application in promoting CIK cell in-vitro proliferation. The total content of rehmannin B, rehmannin D and jioglutin A in the extract is not lower than 90%. The preparation method includes the steps that S1, fresh rehmannia slices are subjected to heat reflux extraction by using an ethanol aqueous solution and subjected to vacuum concentration to obtain an extract; S2, the extract is dissolved with water, extracted with petroleum ether to remove impurities and then extracted with dichloromethane, and dichloromethane extraction solutions are combined, concentrated and dried to obtain a dichloromethane extract; S3, the dichloromethane extract is loaded on an XDA-1B macroporous adsorption resin column, eluted with 30% ethanol to remove impurities and then eluted with 75% ethanol, and 8-10 BV of eluent is collected, concentrated and dried to obtain a crude product of therehmannia extract; S4, the crude product of therehmannia extract is loaded to a normal-phase silica column and eluted with a dichloromethane / methanol / isopropanol mixed solvent in the volume ratio of 10:1:0.2, and 6-7 BV eluent is collected, concentrated and dried. The extract can promote CIK cell in-vitro proliferation.

The invention belongs to the field of pesticides, and relates to a microemulsion containing ethylicin and hymexazol. The microemulsion contains an organic solvent and a surfactant besides ethylicin and hymexazol, wherein the mass part ratio of ethylicin to hymexazol is 1:5 to 5:1, the total mass of ethylicin and hymexazol accounts for 1-70% of the total mass of the microemulsion, the amount of theorganic solvent is 1-80%, the amount of the nonionic surfactant is 1-30%, the organic solvent is selected from one or more of ethanol and isopropanol, the surfactant is selected from a mixed solutionof No.1602, and No.601 or No.602 or No.1601 in pesticide emulsifiers, or a mixed solution of No.700, and No.601 or No.602 or No.1601 in the pesticide emulsifiers. The microemulsion is used for seed dressing, soil mixing, spraying and root irrigation, and is used for preventing and treating bakanae disease, damping off, rotten seedling disease, blast, ring spot and rice blast of plants.

The invention belongs to the technical field of traditional Chinese medicine detection, and particularly provides a method for simultaneously identifying components of rhizoma coptidis and cortex phellodendri, which comprises the following steps: (1) preparing a test solution, a reference solution and a reference medicinal material solution; (2) thin layer chromatography detection: carrying out two-time expansion and respective inspection, wherein butyl acetate-methanol-isopropanol-water is adopted as a developing solvent for first-time developing, n-butanol-water-acetic acid is adopted as a developing solvent for second-time developing, a'secondary thin-layer developing method ' is utilized for the first time, the developing solvent with specific composition is adopted, the same thin-layer plate is adopted, and second-time sample application is not needed; the method can be used for simultaneously and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, avoids the increase of resources and cost caused by independently and qualitatively identifying the components of rhizoma coptidis and cortex phellodendri, ensures the uniformity, stability and reliability of quality, and has the advantages of high accuracy, time and cost saving and easiness in popularization and application.