Preparation method of cell matrix-free material for porcine adipose tissue

Abstract

The invention discloses a method for preparing a cell matrix-free material for a porcine adipose tissue. The method comprises the following steps: (1) taking fresh porcine subcutaneous fats, cleaning, cutting into pieces, adding water or normal saline, homogenating, and centrifuging to obtain a precipitate, and rinsing by PBS solution or water; (2) crushing the precipitate, rinsing by the SDS solution, then rinsing by the PBS solution or water, and centrifuging to obtain a precipitate; and (3) rinsing the precipitate by isopropanol solution, and then rinsing by the PBS solution or water to obtain the product. The acellular matrix material prepared by the method disclosed by the invention is thorough in decellularization, low in immunogenicity, good in biocompatibility, simple in preparation method, low in cost and good in application prospect.

Description

technical field [0001] The invention relates to a preparation method and application of a porcine adipose tissue acellular matrix material, belonging to the field of biological materials. Background technique [0002] In recent decades, the incidence of female breast cancer in my country has continued to soar, and it has become the largest malignant tumor among women in my country. At the same time, soft tissue defects of different degrees can also be caused by diseases such as skin and soft tissue tumors, trauma, congenital deformities, deep burns, and chronic ulcers. , directly affect the patient's appearance and function, and have a direct impact on the patient's quality of life; in addition, there is a strong demand for aesthetic remodeling of the human body: according to the statistics of the American Society of Plastic Surgeons, in 2012, a total of 286,000 breast augmentation operations were performed in the United States. It ranks first in plastic surgery in the United ...

AI Technical Summary

Problems solved by technology

Among them, the non-ionic detergents in the physical and chemical methods are blown off, hypotonic and hypertonic solutions are relatively mild, and have less impact on the extracellular matrix, but the decellularization is not complete; in the chemical methods, the ionic detergents can Effective decellularization, but has an impact on the extracellular matrix; and enzymatic treatment can effectively decellularize, and has little impact on the extracellular matrix, but it is expensive, high cost, and difficult to industrial application.

[0006] Due to the particularity of adipose tissue, the currently reported adipose decellularization methods all use trypsin and DNase to treat them, which can effectively decellularize and maintain the structure of the extracellular matrix, but the enzymes are expensive, resulting in high production costs and difficult For large industrial applications, at the same time, the entire processing time is too long, usually 5 days or more

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