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719 results about "Potassium acetate" patented technology
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Potassium acetate (CH₃COOK) is the potassium salt of acetic acid.
High performance water-based mud system
InactiveUS20050187113A1Premature consumptionEffectively stopFluid removalFlushingWater basedPolystyrene
A drilling fluid or mud additive having several components, including leonardite (humic acid); potassium acetate; partially hydrolyzed polyacrylamide (PHPA); polyanionic cellulose polymer (PAC); sulfonated asphalt; sulfoalkylated tannin; polystyrene maleic anhydride copolymer; micronized cellulose fiber; and calcium carbonate. These components are preferably premixed in a dry formulation as a powder or as pellets, and shipped to the site in bags or bulk tanks. This offers substantial advantages over the prior art in that the drilling mud of the present invention may be formed simply by adding the dry mix to a predetermined amount of water, and is thus much easier to make than the prior art wet mix drilling fluids, which typically require precise ratios of several different powders and liquids to be mixed together. Hydration buffers help keep the powder or pellets dry and flowable.
Owner:HAYES JAMES R
Water soluble fertilizer containing high-concentration humic acid and preparation method thereof
ActiveCN103408373AImprove buffering effectReduce heavy metal contentFertilizer mixturesDipotassium hydrogen phosphateHigh concentration
The invention provides a water soluble fertilizer containing high-concentration humic acid. The water soluble fertilizer comprises, by weight, 15-25 parts of a water soluble humic acid salt, 18-25 parts of urea, 9-15 parts of dipotassium hydrogen phosphate, 8-13 parts of potassium acetate, 0.5-2 parts of borax, 0.1-10 parts of polyglutamic acid, 1-5 parts of rhamnolipid, 5-10 parts of a hydroxymethyl cellulose aqueous solution and 30-40 parts of water. The water soluble fertilizer has humic acid content greater than or equal to 150g / L. The invention also provides a preparation method of the water soluble fertilizer containing high-concentration humic acid. The preparation method comprises the following steps of 1, carrying out heating stirring of the water soluble humic acid salt and water to obtain a humic acid salt solution, 2, adding dipotassium hydrogen phosphate, potassium acetate, borax, polyglutamic acid, rhamnolipid and urea into the humic acid salt solution, and carrying out heating stirring to obtain a mixed solution, and 3, carrying out emulsification of the hydroxymethyl cellulose aqueous solution and the mixed solution to obtain the water soluble fertilizer containing high-concentration humic acid. The water soluble fertilizer has obvious fertilizer efficiency, is environmentally friendly, stable and uniform and has a wide application range.
Owner:陕西鼎天济农腐殖酸制品有限公司
Kit and method for extracting microbial DNA
ActiveCN101935647AImprove bindingReduce adsorptionDNA preparationSodium acetateAluminum ammonium sulfate
The invention relates to the field of molecular biology and discloses a kit and a method for extracting microbial DNA. The kit comprises lysis solution, inhibitor removal solution and binding solution, wherein the lysis solution comprises 50 to 200mM of Tris-HCl, 50 to 150mM of EDTA, 0.5 to 3M of NaCl, 0.5 to 2 percent of CTAB, 0.5 to 2 percent of PVP and 0.5 to 2 percent of SDS; the inhibitor removal solution comprises 100 to 300mM potassium acetate, sodium acetate or ammonium acetate and 50 to 200mM aluminum sulfate, ammonium sulfate or aluminum ammonium sulfate; and the binding solution comprises 3 to 6M guanidine hydrochloride, 10 to 50mM Tris-HCl and 5 to 50 percent isopropanol. The kit and the method for extracting the microbial DNA have the advantages of high purity, high universality, high extraction speed, direct use for a downstream experiment, and application to extracting DNA from a microbe-containing sample.
Owner:中生方政生物技术股份有限公司
Puncture sealing agent for a tire, and process for producing the same
Owner:ITW GLOBAL TIRE REPAIR INC
Chain-prolonged type fluorenyl bimaleimide and its preparation method
The invention discloses a making method of new chain-extending typed fluorenyl dimaleimide, which comprises the following steps: dissolving 9, 9-di(4-amino phenyl) fluorene into N, N-dimethyl acetamide; stirring; dripping maleic anhydride of N, N-dimethyl acetamide with the same molar weight to react for 2h; adding dianhydride directly with the same molar weight; stirring 3h; adding the compound of acetic anhydride and potassium acetate; stirring under indoor temperature to react for 4h; sedimenting in the water; obtaining the product with receiving rate over 95%.
Owner:FUDAN UNIV
Fire extinguishing composition
Owner:KIDDE FENWAL INC
Self-humidifying proton exchange membrane, membrane-electrode assembly, and fuel cell
ActiveUS20070092777A1Stay hydratedFuel cells groupingElectrolytic capacitorsIridiumChemical reaction
A self-humidifying proton exchange membrane (PEM) composition, a membrane-electrode assembly, and a fuel cell. The PEM composition comprises (a) a proton-conducting polymer; (b) a catalyst that promotes the chemical reaction between hydrogen and oxygen molecules to generate water in the membrane, and (c) a deliquescent material dispersed in this polymer. The amount of catalyst is preferably 0.01%-50% by weight on the basis of the polymer weight. The catalyst is preferably a metal catalyst selected from the group consisting of platinum, gold, palladium, rhodium, iridium, ruthenium, and mixtures and alloys thereof. Suitable deliquescent materials include, but are not limited to, calcium chloride, calcium bromide, potassium biphosphate, potassium acetate and combinations thereof. A deliquescent material absorbs and retains an essentially constant amount of moisture to keep the proton mobile in the PEM structure. The fuel cell containing such a PEM composition can be used in a higher temperature and / or lower-humidity environment.
Owner:NANOTEK INSTR GRP LLC
B1-level spray coating type polyurethane modified polyisocyanurate foams
The invention relates to B1-level spray coating polyurethane modified poly (isocyanuric acid ester) foam, which relates to a polyurethane material. The B1-level spray coating polyurethane modified poly (isocyanuric acid ester) foam is characterized in that: the compositions of the polyurethane rigid foam by weight proportion are 50 to 70 kilograms of polyester polyol PS-2412, 35 to 20 kilograms of polyether glycol 4110, 15 to 10 kilograms of polyether glycol 403, 6 to 7 kilograms of crosslinking agents, 1.5 to 2 kilograms of surfactants, 0.5 to 1 kilogram of water, 2 to 3 kilograms of composite amine catalysts, 1 to 2.5 kilograms of alkali metal salt catalysts (potassium acetate), 0.5 to 1 kilogram of PZA (six-hydrogen triazine), 23 to 24 kilograms of composite fire retardant (DMMP / TCPP) and 35 to 38 kilograms of foaming agents 141b; the compositions are uniformly mixed, and 210 to 212 kilograms of PAPI isocyanate solvent are taken by weight ratio; and the mixture and the isocyanate solvent are thrown into a spray gun through two pumps and mixed, and then spray coating is performed. The B1-level spray coating polyurethane modified poly (isocyanuric acid ester) foam has the advantages that: the polyester polyol PS-2412 is selected and a proper mixture ratio of DMMP and TCPP of the composite fire retardant is selected, thereby the fire retardant property of the polyurethane rigid foam reaches the B1 level.
Owner:福建省新达保温材料有限公司
Baicalin metal complex and preparation method and application thereof
InactiveCN102516341AReduce pollutionAvoid it happening againAntibacterial agentsGroup 1/11 organic compounds without C-metal linkagesDipotassium hydrogen phosphateSodium acetate
The invention discloses a baicalin metal complex of which the molecular formula is (C21H17O11) xM (H2O) y, wherein M in the formula is Cu (II), x is 1 and y is 2; or M is Fe (III), x is 2 and y is 0; or M is La (III) or Y (III), x is 3 and y is 0. The preparation method comprises the following steps: adding aqueous alkali into baicalin until the baicalin and alkali (sodium bicarbonate, potassium bicarbonate, sodium formate, potassium formate, sodium acetate, potassium acetate, disodium hydrogen phosphate, dipotassium hydrogen phosphate, sodium phosphate or potassium phosphate) are just completely reacted, then adding metal salt (copper salt, ferric salt, lanthanum salt or yttrium salt) for reaction to obtain the baicalin metal complex. The baicalin metal complex has obviously stronger antibacterial and antitumor activities than the baicalin, can be used for preparing antibacterial drugs and antitumor drugs, and has good development and application prospects. In the preparation method, an organic solvent is not used, a strong basic condition is not adopted, and the method is simple and feasible, is green and environment-friendly and has the advantages of low cost, high product purity and high yield.
Owner:SOUTHWEST UNIVERSITY
Nano stripping method for coal series hard kaolinite
InactiveCN101121828AReduce energy consumptionReduce pollutionInorganic pigment treatmentSilicon compoundsCavitationKaolinite
The present invention discloses a coal hard kaolinite nano-stripping method. The hard kaolinite powder (the particle size of 10 to 20 microns) is used as the raw material; the water and dispersant are added to make the pulp; then the oxidant is added to remove the carbon for purification; then the mixture is put into the saturated solution of potassium acetate; the potassium acetate is used as the intercalation agent; the intercalation layer is made between the kaolinite layers to form the kaolinite-potassium acetate intercalation complex; the power ultrasonic acoustic cavitation is used to strip the intercalated kaolinite; then the surfactant is added to decorate the surface of the stripped kaolinite; finally, the materials are dried and broken to make the nano-kaolinite. The average thickness of the nano-kaolinite chip is between 20 and 60 nanometers; the average particle size of the chip is between 300 and 500 nanometers.
Owner:HEBEI UNIV OF ENG
Aqueous foaming fire extinguishing composition
The present invention relates to a novel fire extinguishing composition suitable for use and storage at a temperature below -1° C., said composition comprising an aqueous solution of:v) 50-60% by weight of 60% aqueous solution of potassium acetate,vi) 7-10% by weight of a 3% solution of AFFF,vii) 15-20% by weight alkylene glycol selected from the group consisting of ethylene glycol and propylene glycol, andviii) balance being water.
Owner:TYCO FIRE PRODS LP
Acetylene method vinyl acetate catalyst and preparation method thereof
InactiveCN104437627AUniform load carrierStable load carrierOrganic compound preparationOrganic-compounds/hydrides/coordination-complexes catalystsActivated carbonPtru catalyst
The invention relates to a vinyl acetate catalyst, a preparation method and a vinyl acetate synthesis method which are mainly used for solving the problem of low catalyst activity in the prior art. In order to better solve the problem, the technical scheme adopted by the invention is as follows: the vinyl acetate catalyst provided by the invention comprises a carrier, 50-200 g / L zinc acetate and 0-10 g / L potassium acetate; the preparation method comprises the following steps: (a) performing microwave treating on activated carbon under a non-oxidizing atmosphere relative to the activated carbon to obtain the carrier; (b) loading the necessary dosage of zinc acetate and a cocatalyst by an impregnation method; (c) drying to obtain the catalyst. The vinyl acetate catalyst can be used in the industrial production of acetylene method vinyl acetate synthesis.
Owner:CHINA PETROLEUM & CHEM CORP +1
Environment-friendly high-density solid-free weighting agent and preparation method
ActiveCN104610937AWide range of densitiesReduce pollutionDrilling compositionCarboxylic acidPotassium formate
An environment-friendly high-density solid-free weighting agent comprises components in parts by weight as follows: 0.02-0.05 parts of a dispersant, 0.3-1 part of a tackifier, 0.5-1.2 parts of chelating agent, 0.6-2 parts of a corrosion inhibitor and the balance of soluble weighting materials, wherein the dispersant is one or two of sodium polyacrylate and copolymer of phosphono and carboxylic acid; the tackifier is one or more of chitosan, agar, polyacrylamide, xanthan gum and hydroxypropyl methyl cellulose; the chelating agent is one or two of aminophosphonic acid chelate resin and ethylenediamine tetraacetic acid disodium; the corrosion inhibitor is one or two of multiple polycarboxylate and sodium gluconate; the soluble weighting materials are one or more of potassium formate, potassium citrate, potassium acetate, potassium silicate and potassium sulfate. The process steps are as follows: the raw materials are taken in weight proportion, mixed and uniformly stirred; the raw materials are added to water and dissolved at the high temperature of 70-80 DEG C; a product is evaporated to dryness, and solid is obtained; the obtained solid is ground into powder, and the solid weighting agent is obtained.
Owner:成都西油华巍科技有限公司
Preparation methods of 2,5-furandicarboxylic acid and 2,5-furanyl polyester
InactiveCN108997278ALow costLow priceOrganic chemistryBulk chemical productionSodium bicarbonatePolyester
The invention provides a preparation method of 2,5-furandicarboxylic acid. The preparation method comprises the following steps: A), with non-grain biomass as a raw material, preparing furfural; B), oxidizing the furfural to obtain furoic acid; C), performing an addition reaction on the furoic acid and carbon dioxide under the action of a catalyst to obtain the 2,5-furandicarboxylic acid, whereinthe catalyst is one or more of potassium carbonate, sodium oxalate, sodium nitrate, potassium nitrate, sodium nitrite, potassium nitrite, potassium acetate, sodium acetate, potassium formate, sodium formate, sodium hydrogen carbonate, potassium hydrogen carbonate, sodium hydroxide and potassium hydroxide. The furfural is prepared from the non-grain biomass as the raw material, so that the raw material cost is low; the furoic acid reacts with the carbon dioxide under the action of the specific catalyst to obtain the 2,5-furandicarboxylic acid; the catalyst is low in price; the used carbon dioxide is environment-friendly; by the preparation method, the yield is high.
Owner:芜湖万隆新材料有限公司
Water system electrolyte and application thereof
ActiveCN108630458AInhibit electrolysisImprove electrochemical performanceHybrid capacitor electrolytesSecondary cellsHigh concentrationDecomposition
The invention discloses a water system electrolyte and application thereof. The water system electrolyte comprises 40%-85% of potassium acetate by weight. The electrolyte is added with high concentration of potassium acetate, so that almost all water molecules can be hydrated with potassium ions, the water molecules are tightly connected with the potassium ions via a Van der Waals' force, hydrolysis of the water molecules in the surface of an electrode is inhibited, and thus, the decomposition voltage is improved, the service life of an electrochemical energy storage device is prolonged, the capacity maintaining rate and the electrochemical performance of the electrochemical energy storage device are improved, and the water system electrolyte and application thereof have great significancein research and application in the energy storage field of the water system.
Owner:PEKING UNIV SHENZHEN GRADUATE SCHOOL
Method for simultaneously extracting DNA (deoxyribonucleic acid) and RNA (ribonucleic acid) from lily tissue
InactiveCN102776174AResolve interferenceQuality improvementDNA preparationSodium acetateHigh concentration
The invention discloses a method for simultaneously extracting DNA (deoxyribonucleic acid) and RNA from lily tissue. The method comprises the following steps of: obtaining total nucleic acid solution through crude extraction and purification of the same steps from a lily tissue sample; and then sequentially selectively precipitating RNA and DNA, thereby obtaining high-quality DNA and RNA. The method disclosed by the invention is short in time, economic and fast, and good in stability; and the extracted nucleic acid is high in quality. The method has the characteristics that high-concentration potassium acetate is utilized for precipitating polysaccharides twice, so that the polysaccharides in the lily sample can be effectively removed; and in the DNA and RNA separation process, the RNA is selectively precipitated by means of the synergistic effect of lithium chloride and absolute ethyl alcohol and then the DNA is precipitated by using sodium acetate and isopropanol; and therefore, the efficiency of the DNA-RNA separation is high, the loss of the nucleic acid is low and the precipitation time is greatly shortened. The method is suitable for simultaneously extracting the DNA and the RNA from the lily tissue containing rich polysaccharides and other secondary metabolites.
Owner:KUNMING UNIV OF SCI & TECH
Preparation method of catalyst for synthesizing vinyl acetate by virtue of acetylene method and synthetic method of vinyl acetate
InactiveCN104549498AUniform load carrierStable load carrierOrganic compound preparationOrganic-compounds/hydrides/coordination-complexes catalystsActivated carbonPolymer science
The invention relates to a preparation method of a catalyst for synthesizing vinyl acetate by virtue of an acetylene gas phase method, the catalyst and a synthetic method of vinyl acetate. According to the preparation method of the catalyst, the catalyst and the synthetic method of vinyl acetate, the problem of low catalyst activity in the prior art is mainly solved. The catalyst comprises a carrier, 50g / L-200g / L zinc acetate and 0-10g / L of potassium acetate. The preparation method comprises the following steps: (a) radiating the activated carbon by virtue of 60 Co-gamma ray to obtain the carrier; (b) loading required amounts of zinc acetate and potassium acetate by virtue of a dipping method; and (c) drying to obtain the catalyst. According to the technical scheme, the problem is well solved. The catalyst can be applied to the industrial production for synthesizing the vinyl acetate by virtue of the acetylene method.
Owner:CHINA PETROLEUM & CHEM CORP +1
Environment-friendly snow melting agent and preparation method thereof
ActiveCN103342988AReduce manufacturing costSimple production processOther chemical processesSnow meltingEthylic acid
The invention relates to an environment-friendly snow melting agent and a preparation method of the snow melting agent, and belongs to the field of the snow melting agents. The snow melting agent is prepared from acetates and chlorine salts with low corrosivity as the main raw materials by mixing acetates including potassium acetate, magnesium acetate and calcium acetate with calcium chloride and magnesium chloride in a certain proportion, as well as a small quantity of corrosion inhibitor. The common fault that a common inorganic snow melting agent is high in corrosivity is effectively overcome, the cost is reduced, and therefore the snow melting agent is suitable for promotion and application.
Owner:SHENYANG POLYTECHNIC UNIV
Ruthenium system ammonia synthesis catalyst using graphitization activated carbon as carrier and preparation method of catalyst
ActiveCN104084197AImprove thermal stabilityImprove resistance to methanationMetal/metal-oxides/metal-hydroxide catalystsBulk chemical productionActivated carbonPotassium nitrate
The invention discloses a ruthenium system ammonia synthesis catalyst using a graphitization activated carbon as a carrier and a preparation method of the catalyst. The ruthenium system ammonia synthesis catalyst is as shown in Ru-X-K / AC, wherein Ru represents that a precursor is a water-soluble chloride-free ruthenium complex or ruthenium chloride; X represents a metal adjuvant and is one or more of nitrate, acetate, carbonate, metallic oxide and hydroxide of rare-earth metal or alkaline-earth metal; AC represents a graphitization activated carbon carrier, and K represents a kalium adjuvant, namely potassium hydroxide, potassium nitrate, potassium acetate and potassium carbonate; the graphitization activated carbon which has the advantages of high specific surface area, high graphitization and low oxygen content is utilized as the carrier, and the ammonia synthesis catalyst is prepared through steps such as secondary graphitization of graphitization activated carbon, preparation of precursor and preparation of catalyst. The ruthenium system ammonia synthesis catalyst has the advantages that the graphitization activated carbon has large specific surface area and pore volume, enough place is provided for metal segregation, the graphitization degree is high, the oxygen content is low, the methanation resisting capacity of the catalyst is strong, and the stability is good.
Owner:FUZHOU UNIV ASSET MANAGEMENT CO LTD
Preparation method of extra-long manganese dioxide nanowires
ActiveCN103193273AGood modification effectEvenly distributedNanotechnologyManganese oxides/hydroxidesNanowireEthylic acid
The invention discloses a preparation method of extra-long manganese dioxide nanowires. The method comprises using manganese sulfate as a reducing agent, potassium chlorate as an oxidizing agent, and potassium acetate and acetic acid as an additive, performing a hydrothermal reaction for 6-24 hours under 140-200 DEG C in a polytetrafluoroethylene-lined autoclave, naturally cooling, washing and filtering products, and drying to obtain the extra-long manganese dioxide nanowires with a diameter of 50-150 nanometers and a length of more than 100 [mu]m. The method can synthesize the extra-long manganese dioxide nanowires for one step, is relatively low in synthesis temperature and production cost, accords with conditions of large-scale industrial application, and has a wide application prospect.
Owner:GUANGDONG UNIV OF TECH
Method for leaching weathering crust eluvial type rare earth ore with aluminum inhibition
InactiveCN103526014AReduce aluminum ion contentThe leaching rate has no effect onProcess efficiency improvementSodium acetateAluminum Ion
The invention provides an impurity-inhibiting leaching method for inhibiting leaching of aluminum during weathering crust eluvial type rare earth ore leaching process. The method comprises that acetates are added in an ore leaching solution, the ore leaching solution is an ammonium sulfate solution or an ammonium chloride solution or a composite ammonium salt solution composed of the above solutions, the acetates are any one of ammonium acetate, sodium acetate and potassium acetate or any mixture thereof, and the addition amount of the acetates in the ore leaching solution is 0.005wt%-2wt%. In the method, the addition of the aluminum inhibitors can lower the aluminum ion content in the rare earth leaching solution by above 91% effectively, and has no influence on the leaching rate of rare earth, which are beneficial to subsequent processing of the rare earth mother solution. The addition of the aluminum inhibitors can inhibit expansion of clays well. The acetates are non-poisonous and the cost is low.
Owner:WUHAN INSTITUTE OF TECHNOLOGY
Isothermal nucleic acid amplification reaction reagent and isothermal nucleic acid amplification method
ActiveCN102816756AStrong specificityEfficient amplificationDNA preparationRibonucleosidePolyethylene glycol
The invention discloses an isothermal nucleic acid amplification reaction reagent which comprises Tris buffer solutions, potassium acetate, magnesium acetate, dithiothreitol, polyethylene glycol, adenosine triphosphate (ATP), deoxy-ribonucleoside triphosphate (dNTPs), phosphocreatine, creatine kinase, a primer group, single-strand binding (SSD) protein, colon bacillus helicase RecQ protein, UvsY protein and DNA polymerase. The invention further discloses an isothermal nucleic acid amplification method which includes steps of extracting DNA or performing inverse transcription after DNA extracting, and adding the isothermal nucleic acid amplification reaction reagent and reacting the mixture at a temperature in a range between 25 DEG C and 45 DEG C for 10 minutes to 60 minutes to complete the nucleic acid amplification. Compared with traditional polymerase chain reaction (PCR) technologies and according to the technology, nearly no professional instrument is needed, the operation is simple, technical requirements for operators are low, and the reaction time only needs about half an hour.
Owner:JIANGSU QITIAN GENE BIOTECHNOLOGY CO LTD
Manufacturing method of nano-kaolin
InactiveCN1513800ADestroy or weaken the Coulomb forceWeaken the Coulomb forceAluminium silicatesClaywaresChemical reactionThiourea
A process for preparing nano Kaolinite includes adding the chemical assistant (potassium acetate, dimethyl sulfoxide, thiourea, urea, hydrazine, or their derivative) which can decrease the Coulomb force between layers of kaolinite, heating or plasma, ultrasonic or microwave acting for physiochemical reaction, grinding, water washing and drying.
Owner:广东佛陶集团物资工贸有限公司 +1
Polyurethane heat insulation foaming material and preparation method thereof
InactiveCN101787109AHigh compressive strengthHigh viscosityPigment treatment with macromolecular organic compoundsPigment treatment with non-polymer organic compoundsPolyesterPolyol
The invention discloses a polyurethane heat insulation foaming material and a preparation method thereof in the technical field of nano composite materials. The polyurethane heat insulation foaming material comprises the following components in part by weight: 20 to 40 parts of polyether polyalcohol, 60 to 80 parts of aromatic polyester polyalcohol, 300 to 400 parts of polymethylene polyphenyl isocyanate, 0.1 to 0.7 part of carbon nano tube base body, 5 parts of water, 10 parts of flame retardant, 4 to 5 parts of potassium acetate, 3 to 8 parts of triethanolamine, 1 to 3 parts of organic tin and 5 to 10 parts of silicon oil. The compressive strength of the polyurethane foaming material is remarkably improved, the viscosity of a foaming body cannot be remarkably improved, the cell structure of the foaming body is not changed, and the heat insulation performance of the foaming body cannot be reduced; and because the nitrogen-containing isocyanurate ring is introduced into the foaming material, the material has good flame-retardant performance under the condition of using a small amount of flame retardant.
Owner:SHANGHAI JIAO TONG UNIV
Method for synthesizing cholesterol by using pregnenolone as raw material
The invention provides a method for synthesizing cholesterol by using pregnenolone as a raw material. The method comprises the following steps: 1) adding potassium acetate into methyl alcohol, and performing a reaction on sulfonate to obtain 6-methoxyl-3,5-cyclo-5alpha-pregn-20-one; 2) performing a reaction on triphenylphosphine and 1-chloro-4-methylpentane in an aprotic solvent to obtain a 4-methylbutyltriphenyl phosphonium chloride solution; 3) adding potassium tert-butoxide into the 4-methylbutyltriphenyl phosphonium chloride solution, and performing a wittig reaction; 4) under the catalysis of a rhodium catalyst, performing an asymmetric hydrogenation reaction to obtain 6-methoxyl-3,5-cyclo-5alpha-cholestane; 5) performing a catalytic hydrolysis deprotection reaction by using sulfuric acid to obtain the cholesterol. The method provided by the invention has the advantages that six-step reactions in the conventional method are simplified into four-step reactions, and a ring-opening reaction in which a great number of hydrochloric acid and a large number of zinc powder are consumed in a route of using saponin as an initial raw material. The synthesizing method is simple in process, the consumption of the raw material and auxiliary materials is low, and the mole yield is high; the method is economical and environmentally friendly, and facilitates industrial implementation.
Owner:HUNAN KEREY BIOTECH
Extraction method of apostichopus japonicus body-wall total RNA
InactiveCN101864414AMeet Gene Expression AnalysisFulfil requirementsDNA preparationWater bathsTotal rna
The invention discloses a high-extraction-purity, good-integrity and high-yield extraction method of apostichopus japonicus body-wall total RNA (Ribonucleic Acid). The method comprises the following steps of: quickly freezing apostichopus japonicus body-wall tissue in liquid nitrogen; grinding and putting the frozen tissue in lysate to homogenate, centrifugate and take supernatant fluid; adding chloroform to the supernatant fluid and centrifugating to take the supernatant fluid to another centrifuge tube; then, adding a high-salt solution and isopropyl alcohol and filtering precipitation with ethanol; dissolving the precipitation with DEPC (diethypyrocarbonate) treated water to have constant volume; sequentially adding a DNA enzyme buffer solution without RNA enzymes, DNA enzymes without RNA enzymes and an RNA enzyme inhibitor to the dissolved solution to mix; carrying out a water bath at 37 DEG C to obtain DNA lysate; adding phenol and chloroform in a ratio of 5:1 to the DNA lysate to mix, centrifugate and take supernatant fluid; adding a glycogen solution, a potassium acetate solution and pre-cooling ethanol to the supernatant fluid; mixing and staying over night; centrifugating to discard the supernatant fluid; washing and dying the precipitation with ethanol; dissolving the solution with the DEPC treated water to have constant volume of 20 mu l; and storing at 80 DEG C below zero.
Owner:DALIAN OCEAN UNIV
Method for purifying dermatan sulfate from heparin byproduct
The invention discloses a method for purifying dermatan sulfate from a heparin byproduct. The method comprises the following steps of: using the heparin byproduct as a raw material; dissolving the heparin byproduct, and then adding potassium acetate into the dissolved heparin byproduct; adjusting the pH value of solution by using acetic acid to generate a deposit, wherein clear solution is sulfate polysaccharide solution; adding a Ban's agent and saturated sodium hydroxide solution into the sulfate polysaccharide solution; centrifugally collecting the deposit; performing re-precipitation; dissolving the deposit again; washing away copper ions by using a chromatography column with anion exchange properties and sodium chloride solution; performing linear gradient elution by using the sodium chloride solution; collecting eluant; and evaporating and concentrating the eluant, and depositing the eluant by using ethanol to obtain high-purity dermatan sulfate. The method has the advantages of simple purification process, high purification yield, low cost, easy amplification and suitability for industrial production.
Owner:SHENZHEN HEPALINK PHARMA GRP CO LTD
Superfine kaoline lamellar material preparation
InactiveCN1439677AHigh intercalation rateGood peeling effectBenzathrone dyesMaterials preparationPotassium acetate
A superfine kaolin flake material is prepared through mixing potassium acetate as sandwich agent with kaolin grinding, intercalating, laying aside, water washing, centrifugal separating of patassium acetate, stripping kaolin flakes, baking and grinding. Its advantages are high intercalating rate and slake-stripping effect, and low cost.
Owner:EAST CHINA NORMAL UNIVERSITY
Method for extracting RNA in gymnosperm tissue
InactiveCN101492485AHigh purityQuality improvementSugar derivativesSugar derivatives preparationRNA extractionPolyphenol
The invention discloses a method for extracting RNA from gymnosperm tissues. The method includes the following steps: 1) extracting buffer solution by RNA to treat plant materials; 2) protein removal: extracting by equal-volume chloroform / isoamylol for twice; 3) using LiCl to deposit RNA and SSTE to dissolve back RNA; 4) glycan removal: adding absolute ethyl alcohol and potassium acetate to the dissolved solution acquired in step 3) and depositing glycan in ice bath; 5) extracting chloroform / isoamylol again; 6) depositing RNA and DEPC H2O again to dissolve back RNA. In order to remove DNA, the following steps can be also conducted: a) using DNase without RNase pollution to remove DNA; b) using chloroform / isoamylol to extract and remove DNase; (c) repeating step 6). Wherein, RNA extract and buffer solution contains 100mmol / L Tris HCl(pH 8.0), 2g / 100mlCTAB, 2g / 100mlPVP, 2mol / LNaCl and 25mmol / L EDTA and is added with dithiothreitol with final concentration of 10mmol / L and 2 percent (volume percentage) of beta-mercaptoethanol. Therefore, the invention solves the interference of glycan and polyphenol and the like and acquires high-purity RNA.
Owner:BEIJING FORESTRY UNIVERSITY +1
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