79 results about "Dextran sodium sulfate" patented technology

The invention provides an application of codonopsis pilosula polysaccharide as medicine for regulating intestinal flora of human body, namely the application of codonopsis pilosula polysaccharide as medicine, food or health products for preventing colitis. An acute colitis mouse model is established by using fructo-oligosaccharide, codonopsis pilosula polysaccharide and codonopsis pilosula saponinto be respectively prevented in a healthy mouse model for 21 days and adopting 3% dextran sodium sulfate to be induced for 7 days. The remission of three drugs to macroscopical pathological symptomsof colitis is compared by evaluating changes of body weight, colon length, pathological index of disease, histological section and myeloperoxidase activity of experimental mice and the like. The regulation of drug on intestinal flora of colitis mice is investigated by using the Illumina MiSeq 250 sequencing platform based on the variable region of 16S V3-V4, and dominant bacteria in each drug intervention group are identified, which shows the absorption of the highest exposure amount of monomer saponin in codonopsis pilosula saponin in serum and the cecum, clarifies the mechanism that codonopsis pilosula polysaccharide plays a role in the treatment of diseases by changing the action of intestinal flora on saponin metabolism.

The invention discloses application of p-hydroxybenzoic acid in preparing a drug for treating an inflammatory bowel disease. At a dose significantly lower than mesalazine, the p-hydroxybenzoic acid can significantly inhibit the increase in disease activity index scores of enteritis model mice induced by dextran sodium sulfate, improve colon length shortening of the colitis model mice, reduce the colonic MPO activity of the mice, and reduce the inflammatory cell infiltration of colon tissues in the colitis model mice. According to the application, the p-hydroxybenzoic acid can alleviate the inflammatory reaction of the DSS-induced inflammatory bowel disease model mice and improve the severity of the inflammatory bowel disease, and can be used for preparing the drug or a health care productfor treating the inflammatory bowel disease.

The invention relates to recombinant bacillus subtilis capable of synthesizing carotenoid 4,4'-diaponeurosporene with 30 carbon atoms (C30), and belongs to the biotechnology genetic engineering field. A bacillus subtilis expression plasmid pMK3-crtmn (which is capable of expressing C30 carotenoid synthases Crtm and Crtn in bacillus subtilis) containing C30 carotenoid synthase genes crtm and crtn is successfully constructed, and is successfully electro-transformed into bacillus subtilis WB800. The WB800 strain after transformation turns from the original color of white to yellow. A pigment component extracted from the recombinant WB800 is identified as 4,4'-diaponeurosporene by high performance liquid chromatography (HPLC). 4,4'-diaponeurosporene can stimulate maturation of dendritic cells, and generated cytokines (IL-6, IL-10, IL-12p70 and TNF[alpha]) have the amount increased, and moreover and have stronger ability to stimulate proliferation of T lymphocytes. With oral administration of the bacillus subtilis capable of synthesizing 4,4'-diaponeurosporene, the dextran sulphate sodium (DSS)-induced mice colitis symptoms can be significantly reduced. The bacillus subtilis capable of producing 4,4'-diaponeurosporene is expected to be developed as probiotics for prevention of colitis.

The invention provides a preparation method of a hibiscus sabdariffa anthocyanin extract. The extraction process is simple, no pollution is caused, and the anthocyanin content is high. The hibiscus sabdariffa anthocyanin extract prepared by the preparation method disclosed by the invention is wide in application, and can be applied to multiple fields of foods, preparation of health-care products and / or medicines. The invention further provides an application of the hibiscus sabdariffa anthocyanin extract to preparation of a medicine for preventing or relieving colitis. The hibiscus sabdariffa anthocyanin extract has the specific effects on preventing or relieving colitis as follows: the hibiscus sabdariffa anthocyanin extract can relieve weight loss of dextran sodium sulfate induced colitis mice, reduce the inflammation level in serum and tissues, relieve shortening and edema of colon, protect intestinal mucosa barriers, increase the total content of short-chain fatty acids in the intestinal tract, maintain the stable state of intestinal flora, and reduce the abundance of harmful bacteria in the intestinal tract.

The invention discloses an extraction method of a morchella extract. The extraction method specifically comprises the following steps of 1, preparing morchella powder; 2, uniformly mixing a product obtained in the step 1 with normal saline to prepare a morchella mixture; 3, adding an acid solution into the morchella mixture obtained in the step 2, and then adding a pepsase solution into the solution to obtain a morchella in-vitro simulated stomach digest; 4, adding a sodium bicarbonate solution into the morchella in-vitro simulated stomach digest obtained in the step 3, and then adding a trypsin solution into the solution to obtain a morchella in-vitro simulated intestine digest; 5, performing suction filtration and vacuum concentration on the product obtained in the step 4 to obtain the morchella in-vitro simulated digestive juice; and 6, uniformly mixing the morchella in-vitro simulated digestive juice with petroleum ether, and combining petroleum ether extracts to obtain morchella sterol. The morchella extract extracted by the method disclosed by the invention can relieve symptoms of ulcerative colitis induced by dextran sodium sulfate.

The invention discloses a chloroquine-coated denatured albumin nanoparticle for selectively resisting inflammatory cells as well as a preparation method and application thereof, and the denatured albumin nanoparticle is used as a carrier and is coated with a drug chloroquine. The nanoparticles have good stability and biocompatibility, can be efficiently internalized by macrophages, and can effectively aim at inflammatory cells at colon parts, so that inflammation is relieved, and the nanoparticles are used for treating colitis diseases caused by dextran sodium sulfate.

The invention belongs to the technical field of pharmacy, and relates to an application of magnesium isoglycyrrhizinate to preparation of a medicine for treating colitis. In a disease model of mouse acute and chronic enteritis induced by dextran sodium sulfate DSS, the magnesium isoglycyrrhizinate can effectively improve the conditions of weight loss, diarrhea, hemafecia and colonic shortening ofmice, reduce inflammatory cell infiltration and the expression level of inflammatory factors in the colons of the mice, maintain the intestinal epithelial barrier integrity, increase connexins Zo-1, occludin and E-caderin, reduce the Claudin-2 expression level, and reduce fibrosis caused by colitis. It is found for the first time that the magnesium isoglycyrrhizinate can treat colitis, clinical application of the magnesium isoglycyrrhizinate is increased, and remarkable popularization value is achieved.

The invention discloses application of momordica polysaccharide in preparation of a medicine for treating ulcerative colitis and a medicinal preparation thereof, and belongs to the field of biological medicine. The ulcerative colitis is induced by dextran sodium sulfate. Experiments show that the momordica polysaccharide reduces disease activity indexes and improves clinical symptoms by restoring body weight and excrement viscosity and reducing bleeding; an intestinal tract damaged by inflammation is protected by repairing the mucosal barrier function of a colon tissue; and immune system disorders of the ulcerative colitis are ameliorated by attenuating expression of inflammatory cytokines in the colon and reducing recruitment of CD4+T cells. The application field of the momordica polysaccharide is widened, an experimental basis is provided for clinical application of the momordica polysaccharide in preparation of the medicine for treating the ulcerative colitis, and the momordica polysaccharide has a good application prospect and huge potential value and social significance.

The invention discloses an ulcerative colitis preventing baicalin-baicalein composition and a preparation thereof, and belongs to the technical field of traditional Chinese medicines. The compositioncomprises baicalin and baicalein at a mass ratio of 1-10 to 1. According to the invention, a dextran sulphate sodium salt induced ulcerative colitis mice model is adopted, the effect of baicalin-baicalein composition on model mouse is investigated on the aspects of states, pathomorphology and biochemical indicators; experimental results show that compared to baicalin or baicalein used alone, the composition has the good synergistic effect, has the better preventive and therapeutic effect on ulcerative colitis, and can significantly improve symptoms, relieve inflammatory reactions, and promotecolonic mucosal repairing.

The invention relates to a method for preparing a composite heterostructure of zinc oxide nano rods and a silver micron plate. The method includes: utilizing dextran sulphate sodium as regulating agent to prepare a perforated silver micron plate in a reaction system of silver nitrate and an ascorbic acid solution of water and methanamide; depositing zinc oxide seed crystals on the silver micron plate, coating the silver micron plate with the deposition of the zinc oxide seed crystals on an electric-conducting glass sheet in a rotating manner, cleaning, drying, placing the electric-conducting glass sheet in an ethanol solution of hexamethylene tetramine and zinc nitrate to grow a zinc oxide nano rod array on the silver micron plate, fully cleaning with water, and naturally drying to obtain the finished product. The product prepared by the method is a high-level composite heterostructure with the zinc oxide nano rod array growing on the perforated silver micron plate, wherein the diameter of the silver micron plate is about 4 microns, and the size and the structure of the zinc oxide nano rod array are adjustable and controller. The method for preparing the composite heterostructure of the zinc oxide nano rods and the silver micron plate is structurally controllable, good in repeatability and stable in product property, and has excellent photocatalytic performance.