The invention relates to recombinant human
lactoferrin (rhLF) silkworm chrysalis
powder as well as a preparation method and application thereof and belongs to the technical field of biological medicines. A
recombinant virus is constructed mainly by adopting a silkworm chrysalis
baculovirus expression system, the rhLF is expressed with a silkworm chrysalis serving as a
bioreactor, the silkworm chrysalis diseased after being inoculated with the
recombinant virus is prepared into freeze-dried
powder, the activity of the freeze-dried
powder is detected by performing antibacterial experiments
in vitro, and the freeze-dried powder is poured into the
stomach of a mouse to treat the
dextran sulphate sodium (DSS) induced
ulcerative colitis of the mouse. The analysis on the
disease activity index (DAI) of the mouse and the detection on the content of
myeloperoxidase (MPO) in the colonic tissue of the mouse show that the rhLF with
antibacterial activity is expressed successfully in the silkworm chrysalis, and the rhLF silkworm chrysalis powder has a certain effect of treating the DSS induced
ulcerative colitis of the mouse. The preparation method provided by the invention is simple, is low in cost and is used for preparing a novel oral
protein medicine.