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Modified sodium humate and preparation method and application

A technology of sodium humate and humic acid, which is applied in the field of biomedicine, can solve the problems such as the inconspicuous enteritis phenomenon, and achieve the effect of avoiding interference with the action of Escherichia coli, low-concentration intake, and promoting pathological manifestations

Active Publication Date: 2020-11-27
山东亚太海华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, enteritis models are often established by intraperitoneal injection, nasal administration, or oral administration of high-concentration bacterial solutions. Some studies have shown that E. coli K88 alone is used to model enteritis, and the phenomenon of enteritis is not obvious

Method used

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  • Modified sodium humate and preparation method and application
  • Modified sodium humate and preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Embodiment 1: modified sodium humate is prepared through the following steps:

[0023] 1. Weigh 100g of humic acid mineral powder with a mass content of 45% humic acid, 6g of sodium hydroxide, 6g of sodium thiosulfate, add 1000g of distilled water, stir at 60 rpm, react at 80°C for 60 minutes, and centrifuge at 4200 rpm 10 minutes, take the supernatant.

[0024] 2. The supernatant was placed in a stainless steel tray and dried in an oven at 180°C to constant weight to obtain a sodium humate product.

[0025] 3. The content of phenolic hydroxyl group and carboxyl group is determined by non-aqueous conductivity titration method. The optimal titration conditions are that the pH of the sample is 4, the concentration of KOH-isopropanol titration solution is 0.05mol / L, and the rate of nitrogen gas introduction is 80mL / min. The total content of phenolic hydroxyl and carboxyl in the sodium humate product was 129.79 cmol / kg, and the molar ratio of phenolic hydroxyl and carboxyl...

Embodiment 3

[0030] Embodiment 3: The similarity between this embodiment and Embodiment 1 will not be repeated, and the difference is that modified sodium humate is prepared by the following steps:

[0031] 1. Weigh 100g of humic acid mineral powder with a mass content of 45% humic acid, 12g of sodium hydroxide, and 3g of sodium thiosulfate, add 1000g of distilled water, stir at 130 rpm, react at 120°C for 120 minutes, and centrifuge at 4200 rpm for 10 minutes, take the supernatant.

[0032] 2. The supernatant was placed in a stainless steel tray and dried in an oven at 100°C to constant weight to obtain a sodium humate product.

[0033] 3. The total content of phenolic hydroxyl group and carboxyl group in sodium humate products is 128.49 cmol / kg, and the molar ratio of phenolic hydroxyl group to carboxyl group is 2.09.

Embodiment 4

[0034] Embodiment 4: The similarities between this embodiment and Embodiment 1 will not be repeated, and the difference is that modified sodium humate is prepared through the following steps:

[0035] 1. Weigh 100g of humic acid mineral powder with a mass content of 45% humic acid, 11g of sodium hydroxide, and 2 g of sodium thiosulfate, add 1000g of distilled water, stir at 120 rpm, react at 100°C for 80 minutes, and centrifuge at 4200 rpm 10 minutes, take the supernatant.

[0036] 2. The supernatant was placed in a stainless steel tray and dried in an oven at 100°C to constant weight to obtain a sodium humate product.

[0037] 3. The total content of phenolic hydroxyl group and carboxyl group in sodium humate products is 100.17 cmol / kg, and the molar ratio of phenolic hydroxyl group to carboxyl group is 1.59.

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Abstract

The invention discloses modified sodium humate and a preparation method and application thereof. An enteritis model mouse with obvious small intestine inflammation is obtained; Kunming mice drink distilled water containing 3-4% of dextran sodium sulfate for 3-5 days, then 13-15 days later, Kunming mice drink 0.3-0.5% of modified sodium humate aqueous solution and 108 cfu / mL of escherichia coli K88bacterial liquid, alternating is conducted every other day, and an enteritis model is established and treated for 18 days in total; secondly, a sodium humate modification process is controlled; modified sodium humate with a specific molar ratio of phenolic hydroxyl to carboxyl is obtained; the preparation method mainly comprises the following steps of: weighing 100g of humic acid mineral powder with humic acid mass content of 45%, 6-12g of sodium hydroxide and 3-6g of sodium thiosulfate, adding 1000g of distilled water, stirring at 60-130 r / min, reacting at 80-120 DEG C for 60-120 minutes, and centrifuging at 4,200 r / min for 10 minutes; and putting a supernatant into a stainless steel tray, and drying in a drying oven at 80-180 DEG C until the weight is constant, thereby obtaining the modified sodium humate product.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to a modified sodium humate and its preparation method and application. Background technique [0002] Escherichia coli diarrhea is one of the common infectious diseases with high morbidity and mortality in animal husbandry. Although antibiotics are commonly used to treat the disease, long-term use can lead to resistance in bacteria. Therefore, the establishment of enteritis model is helpful for the study of its pathogenesis and the development of new drugs. Since Escherichia coli K88 is an opportunistic pathogenic bacterium, in the process of establishing a model with Escherichia coli K88, problems such as indistinct symptoms of enteritis often occur. Sodium dextran sulfate is a common chemical substance used to establish colitis models and is mainly used for the establishment of colitis models. In the prior art, enteritis models are often established by intraperitoneal injection, nasa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08H7/00C07G99/00A61P1/12A61P1/00A61K49/00A01K67/02
CPCC08H6/00A61K49/0008A61P1/00A61P1/12A01K67/02A01K2267/03A01K2227/105C07G99/00
Inventor 张杰陈清双王瑞明朱小玲丛晓燕位宾
Owner 山东亚太海华生物科技有限公司
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