Method for building dextran sodium sulphate (DSS) induced enteritis mouse model

A mouse model, a technology for establishing a method, applied in the biological field, can solve the problems of model application limitation, weakened pathological response, etc., and achieve a stable and reliable effect of the model

Active Publication Date: 2012-12-12
辉源生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Reducing the concentration of dextran sodium sulfate can avoid animal death, but the pathological response is weakened at the same time, which limits the application of the model

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Establish the mouse model with the method of the prior art mentioned in the background technology, every one or two days, change the freshly prepared dextran sodium sulfate solution and allow the mice to drink for six days, and the dextran sodium sulfate solution does not have Protect from light, and then drink with normal drinking water for four days.

[0023] With the method of the present invention, let the mouse drink the dextran sodium sulfate solution that the weight percentage concentration of one-time preparation is 2% for six days, the drinking water of dextran sodium sulfate has protection from light, then change normal drinking water to drink for four days. sky.

[0024] Use three groups of mice (C57BL / 6, derived from SLACK), each group of 10, the first group is the control group, do not drink dextran sodium sulfate solution; the second group uses the prior art mentioned in the background technology method; the third group uses the method of the present inve...

Embodiment 2

[0026] Using the method of the present invention, mice are allowed to drink dextran sodium sulfate solutions with different weight percentage concentrations, which are respectively: 1.5%, 2%, 3% and 4%. The experimental results showed that the 3% and 4% dextran sodium sulfate solutions by weight percentage caused the mice to lose more than 20% of their body weight, indicating that the toxicity was too high. However, the pathological manifestation of enteritis caused by the dextran sodium sulfate solution with a concentration of 1.5% by weight is too slight. The dextran sodium sulfate solution with a concentration of 2% by weight can reduce body weight by less than 10%, the pathological score of intestinal slices is higher than that of other groups, and the degree of intestinal edema is also higher than that of other groups. This shows that the pathological manifestation of enteritis caused by the dextran sodium sulfate solution with a concentration of 2% by weight is the most ...

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Abstract

The invention provides a method for building a dextran sodium sulphate induced enteritis mouse model. The method includes the following steps: A, choosing female C57BL/6 mice with the weight of 19-21g as the objects to build the mouse model; B, dissolving dextran sulphate sodium salt solution in disinfected drinking water to prepare dextran sulphate sodium salt solution with the weight percentage concentration of 2%, and placing the solution in a water bottle for protecting away from the sun, wherein the solution is prepared when required; C, feeding the chosen C57BL/6 mice in a storageprotect feature (SPF) level animal house, enabling the mice to drink the dextran sulphate sodium salt solution prepared in the step B for six days and then drink the normal drinking water for four days. Accordingly, the chosen C57BL/6 mice are dextran sodium sulphate induced enteritis mouse models. By means of the method for building the dextran sodium sulphate induced enteritis mouse model, death rate of animals is reduced to be zero, and pathology expressions are the same, so that the model is stable, reliable and repeatable.

Description

technical field [0001] The invention relates to biotechnology, in particular to a method for establishing a mouse model of DSS-induced enteritis. Background technique [0002] Ulcerative colitis (UC) has the characteristics of recurrent attacks. During the attack, the patient has ulcerative lesions in the intestinal tract, and abdominal pain, diarrhea or blood in the stool will occur. Existing treatments cannot be effectively applied to all patients, and about 20% of patients eventually require bowel resection. [0003] In the process of new drug development, animal models of ulcerative colitis are essential tools. The mouse model induced by dextran sulfate sodium (Dextran Sulfate Sodium, DSS) is the most widely used animal model, which is an acute ulcerative colitis model. Days, and then change to normal drinking water for four days. Mice developed ulcerative lesions in the gut, including edema, diarrhea, and blood in the stool. In this model, because of the toxicity of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/727A61K49/00A01K67/027
Inventor 王立新
Owner 辉源生物科技(上海)有限公司
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