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Extraction method and application of morchella extract

An extraction method, the technology of Morchella, is applied in the field of inflammatory bowel disease research, which can solve the problems of ulcerative colitis and other problems that have not been seen, and achieve the effects of relieving blood in the stool and weight loss, improving mental state, and relieving liver damage

Active Publication Date: 2021-09-03
XIAN UNVERSITY OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is still a big gap in the research on the physiological activity of Morchella sterols. Up to now, only Morchella sterols can inhibit the proliferation of tumor cells cultured in vitro to varying degrees, but there is no research on Morchella treating ulcers. Related reports on chronic colitis

Method used

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  • Extraction method and application of morchella extract
  • Extraction method and application of morchella extract
  • Extraction method and application of morchella extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A kind of extracting method of hickory chick extract, specifically comprises the following steps:

[0035] Step 1, drying and pulverizing the morel fruiting bodies, and passing through a 40-mesh sieve to obtain the morel powder;

[0036] Step 2, mix the product obtained in step 1 with physiological saline according to the ratio of 1g:15mL to obtain a mixture; place the mixture in a constant temperature magnetic stirrer at 65°C and stir for 60 minutes to obtain a morel mixture;

[0037]Step 3, adding 1mol / L hydrochloric acid to the morel mixture obtained in step 2 to adjust the pH to 2.0, then adding 3mL of pepsin solution with a concentration of 50mg / mL to the solution, avoiding light, charging with nitrogen, and placing it at 37 ℃ constant temperature water bath shaker, the speed is 100rpm / min to digest for 2h, and obtain the in vitro simulated gastric digest of Morchella;

[0038] Step 4, adding 1 mol / L sodium bicarbonate solution to the in vitro simulated gastric di...

Embodiment 2

[0043] A kind of extracting method of hickory chick extract, specifically comprises the following steps:

[0044] Step 1, drying and pulverizing the morel fruiting bodies, and passing through a 40-mesh sieve to obtain the morel powder;

[0045] Step 2, mix the product obtained in step 1 with physiological saline according to the ratio of 1g:10mL to obtain a mixture; place the mixture in a constant temperature magnetic stirrer at 60°C and stir for 30 minutes to obtain a morel mixture;

[0046] Step 3, adding 1mol / L hydrochloric acid to the hickory chick mixture obtained in step 2 to adjust the pH to 2.0, then adding 2mL of pepsin solution with a concentration of 50mg / mL to the solution, avoiding light, charging with nitrogen, and placing it at 37 ℃ constant temperature water bath shaker, the speed is 150rpm / min to digest for 1h, and obtain the in vitro simulated gastric digest of Morchella;

[0047] Step 4, adding 1 mol / L sodium bicarbonate solution to the in vitro simulated g...

Embodiment 3

[0051] A kind of extracting method of hickory chick extract, specifically comprises the following steps:

[0052] Step 1, drying and pulverizing the morel fruiting bodies, and passing through a 40-mesh sieve to obtain the morel powder;

[0053] Step 2, mix the product obtained in step 1 with physiological saline according to the ratio of 1g:25mL to obtain a mixture; place the mixture in a constant temperature magnetic stirrer at 80°C for 45 minutes to obtain a morel mixture;

[0054] Step 3, adding 1mol / L hydrochloric acid to the hickory chick mixture obtained in step 2 to adjust the pH to 2.0, then adding 5mL of pepsin solution with a concentration of 50mg / mL to the solution, avoiding light, charging with nitrogen, and placing it at 37 ℃ constant temperature water bath shaker, the speed is 50rpm / min to digest for 4h, and obtain the in vitro simulated gastric digest of Morchella;

[0055] Step 4, adding 1 mol / L sodium bicarbonate solution to the in vitro simulated gastric dig...

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Abstract

The invention discloses an extraction method of a morchella extract. The extraction method specifically comprises the following steps of 1, preparing morchella powder; 2, uniformly mixing a product obtained in the step 1 with normal saline to prepare a morchella mixture; 3, adding an acid solution into the morchella mixture obtained in the step 2, and then adding a pepsase solution into the solution to obtain a morchella in-vitro simulated stomach digest; 4, adding a sodium bicarbonate solution into the morchella in-vitro simulated stomach digest obtained in the step 3, and then adding a trypsin solution into the solution to obtain a morchella in-vitro simulated intestine digest; 5, performing suction filtration and vacuum concentration on the product obtained in the step 4 to obtain the morchella in-vitro simulated digestive juice; and 6, uniformly mixing the morchella in-vitro simulated digestive juice with petroleum ether, and combining petroleum ether extracts to obtain morchella sterol. The morchella extract extracted by the method disclosed by the invention can relieve symptoms of ulcerative colitis induced by dextran sodium sulfate.

Description

technical field [0001] The invention belongs to the technical field of inflammatory bowel disease research and relates to an extraction method of morel extract. Background technique [0002] Morchella spp. belongs to Ascomycota (Ascomycota), Pezizomycetes (Pezizomycetes), Pezizales (Pezizales), Morchellaceae (Morchella), the earliest record of Morchella in my country Seen in "Compendium of Materia Medica", it records in the Compendium of Mushrooms and Mushrooms in the Ministry of Vegetables: a kind of sheep tripe, with honeycomb eyes, named sheep tripe dish, also known as morel mushroom, wolf tripe dish, honeycomb fungus, grass lily bamboo, It is the collective name for fungi of the genus Morchella. Morchella is delicious and unique in flavor. It not only contains a variety of vitamins, proteins, amino acids, unsaturated fatty acids, mineral elements, selenium and organic germanium necessary for the human body, but also has extremely high nutritional value; it is also rich in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/062A61P1/04
CPCA61K36/062A61P1/04A61K2236/333A61K2236/19A61K2236/35
Inventor 成晓霞姬一凡李欣怡高磊李王丹
Owner XIAN UNVERSITY OF ARTS & SCI
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