The invention discloses an energy-containing fluorescent material rare earth-glutamic acid-imidazole single crystal complex and a preparation method thereof. The complex is prepared from rare earth metal, glutamic acid, imidazole and perchloric acid, the molecular formula of the complex is Ln(Gly)4IM.(ClO4)4 (Ln: Nd, Sm, La, Ce, Pr, Ho, Eu, Er, Lu). The preparation process includes the steps that rare earth oxide and perchloric acid react so that a perchloric acid and rare earth solution can be prepared, glutamic acid is added for reaction, reaction is performed after the pH value is adjusted through an alkaline solution, and crystals are obtained after imidazole is added for reaction and reactants are placed for a period of time. The technology is easy and convenient to perform, ligand selection is scientific, the synthesized rare earth amino acid single crystal complex is clear and novel in structure, and meanwhile the single crystal structure provides a new basic scientific basis for a crystal database. The complex can release a large number of energy in the decomposing process and is a potential energy-containing material. The complex has high fluorescent performance.