The invention relates to a method for separating
cow milk beta-
casein and
whey protein at the low temperature through
microfiltration to simulate composition of human lactoprotein. Skim milk,
whole milk, dried skim milk, concentrated
milk protein powder and the like are taken as raw materials, decalcification pretreatment is performed with the adoption of citrate, glucono-
delta-
lactone and other chelating agents or an
acidifier,
microfiltration and filter wash are performed with the adoption of a
ceramic membrane or a
polyethersulfone membrane and the like under the low-temperature (0-15 DEG C) condition, beta-
casein and
whey protein are selectively enriched into a permeating liquid, the permeating liquid is subjected to
spray drying and
dewatering after
lactose and mineral substances are removed through
ultrafiltration and concentration, and high-
protein powder containing rich beta-
casein and
whey protein is prepared. An adopted technical process has the characteristics of being environment-friendly, efficient, high in
operability, good in safety and the like and is suitable for large-scale industrial production. The obtained compound
protein powder can serve a novel functional lactoprotein ingredient to replace whey
protein and is used for research and development of infant food such as formula milk powder and the like, so that the protein molecule composition of the compound protein powder is closer to that of human lactoprotein.