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Method for promoting microbes to synthesize docosahexaenoic acid (DHA) by exogenous additive factor

A technology for docosahexaenoic acid and microbial synthesis, applied in the biological field, can solve the problem of not improving the potential of microbial synthesis of DHA from the source, and achieve the effects of improving the level of biosynthesis, increasing the percentage content, and improving the metabolic process

Active Publication Date: 2012-07-04
NANJING TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, although the above-mentioned methods have improved the synthesis ability of DHA to some extent, they have not improved the potential of microorganisms to synthesize DHA from the source, especially by improving their metabolic processes to promote the synthesis of DHA by microorganisms. There are reports of promoting microbial synthesis of DHA by simply adding exogenous substances

Method used

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  • Method for promoting microbes to synthesize docosahexaenoic acid (DHA) by exogenous additive factor
  • Method for promoting microbes to synthesize docosahexaenoic acid (DHA) by exogenous additive factor
  • Method for promoting microbes to synthesize docosahexaenoic acid (DHA) by exogenous additive factor

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Experimental program
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Effect test

Embodiment 1

[0032] Before fermentation, a certain amount of acetic acid was added to the fermentation medium of Schizochytrium and Thraustochytrium respectively. Utilize the biosensor to measure the glucose concentration in the culture medium, stop fermentation when the residual glucose concentration in the culture medium is 0g / L.

[0033] The experimental results are shown in Tables 1 and 2:

[0034] Table 1 Effect of exogenous acetic acid on the fermentation of Schizochytrium

[0035]

[0036] Table 2 Effect of exogenous acetic acid on the fermentation of Thraustochytrium

[0037]

[0038]Acetic acid is the direct source of acetyl-CoA, and the amount of acetyl-CoA is directly related to the ability of fatty acid synthesis. It can be seen from Tables 1 and 2 that with the increase of acetic acid concentration, each parameter showed a trend of first increasing and then decreasing. When the concentration of acetic acid is 3mM~6mM, it is beneficial to the biosynthesis of DHA. Espe...

Embodiment 2

[0040] Considering that with the consumption of glucose, the precursors of fatty acid synthesis are also reduced. In order to further increase the production of DHA, when no acetic acid is added at the beginning of the fermentation or the acetic acid is added at the optimal concentration, in the later stage of fermentation, mainly Refers to adding acetic acid to the fermentation broth when the glucose concentration is 20-25g / L, using a biosensor to measure the glucose concentration in the culture medium, and stopping the fermentation when the residual glucose concentration in the culture medium is 0g / L. The results are shown in Tables 3 and 4 below:

[0041] Table 3 Effect of precursor acetic acid on the fermentation of Schizochytrium at the later stage of fermentation

[0042]

[0043] Table 4 The effect of precursor material acetic acid on the fermentation of Schizochytrium

[0044]

[0045] Note: a means adding before fermentation; b means adding in late fermentation...

Embodiment 3

[0048] Before fermentation, a certain amount of simvastatin was added to the fermentation medium of Schizochytrium and Cryptodinoflagellate respectively. Utilize the biosensor to measure the glucose concentration in the culture medium, stop fermentation when the residual glucose concentration in the culture medium is 0g / L. The results are shown in Tables 5 and 6.

[0049] Table 5 Effect of exogenous simvastatin on the fermentation of Schizochytrium

[0050]

[0051] Table 6 The effect of exogenous simvastatin on the fermentation of Cryptidinosa

[0052]

[0053] Acetyl-CoA is a common precursor of mevalonate pathway and fatty acid synthesis pathway, and simvastatin is an inhibitor of the key enzyme HMG-COA reductase in the mevalonate pathway. When an appropriate amount of simvastatin, mevalonate The pathway is inhibited so that more acetyl-CoA flows to the synthesis of fatty acids, which is more conducive to the synthesis of DHA. As shown in Tables 5 and 6, 0.5-4 μM o...

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Abstract

The invention discloses a method for promoting microbes to synthesize docosahexaenoic acid (DHA) by an exogenous additive factor. The method comprises the following steps of: inoculating the microbes into a fermentation culture medium for fermentation to synthesize the DHA, adding the exogenous additive factor into the fermentation culture medium before the fermentation synthesis, and / or adding aprecursor matter for synthesizing fatty acid during the fermentation synthesis, wherein the microbes may be any one of thraustochytrium, schizochytrium and crypthecodium cohnii; the exogenous additive factor is any one or the combination of several of acetic acid, citric acid and simvastatin; and the precursor matter for synthesizing the fatty acid is the acetic acid. In the invention, under simple and effective fermentation control, substrate conversion efficiency is improved, operation is simple, no extra labor and equipment are added, and the synthesizing concentration and production strength of a target product can be improved by just relatively low additive investment, so that production cost is reduced. And the method is pollution-free, increases no manpower and material resources, reduces the cost, is simple and convenient and creates economical benefit.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for promoting the synthesis of docosahexaenoic acid by microorganisms by adding exogenous factors. Background technique [0002] Docosahexaenoic acid (DHA) is an important omega-3 long-chain polyunsaturated fatty acid, commonly known as "brain gold", which has the functions of promoting the growth and development of brain cells, lowering blood fat, lowering blood sugar, protecting eyesight, anti-cancer and Improving immunity and other important physiological functions, known as a new generation of functional health care factors, has attracted great attention from the world. The DHA extracted from traditional deep-sea fish oil is unstable due to the influence of fish species, seasons and geographical location, and the content of cholesterol and other unsaturated fatty acids is high, resulting in limited DHA production, difficult separation and purification, and high cost. With...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645C12R1/89
CPCC12P7/6427
Inventor 黄和魏萍纪晓俊任路静肖爱华仝倩倩
Owner NANJING TECH UNIV
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