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Method for in-situ crystallized synthesis of small-grain ZSM-5 molecular sieve by using direct method
ActiveCN103253684AThe synthesis process is simpleLarge specific surface areaPentasil aluminosilicate zeoliteMicrosphereCrystallinity
The invention provides a method of crystallized preparation of a ZSM-5 molecular sieve in-situ by using a direct method without a template. The method comprises pre-treating high-temperature-roasted kaolin microspheres containing a characteristic structural unit of a five-membered ring by using sodium silicate, adding acid to prepare a reaction mixture, and performing hydrothermal crystallization to obtain in-situ products containing the ZSM-5 molecular sieve and ex-situ ZSM-5 molecular sieve. The kaolin microsphere in-situ crystallized ZSM-5 molecular sieve has a relative crystallinity being capable of being adjusted within 65 % according to requirements, has a crystal size being 0.1-3 [mu]m, is good in wear resistance performance and can be used as a catalytic addictive for increasing propylene yield in a FCC technology. The co-produced ex-situ ZSM-5 molecular sieve has a crystallinity being more than 90 %, and a crystal size of 0.1-3 [mu]m, and can be used for preparing a semi-synthetic catalyst.
Owner:PETROCHINA CO LTD
Method for catalyzing and synthesizing salidroside or analogues by utilizing glucose glycosyl transferase
InactiveCN102174619AHigh selectivityAvoid tedious reaction stepsFermentationLeuconostoc mesenteroidesSalidroside
The invention discloses a method for catalyzing and synthesizing salidroside or analogues by utilizing glucose glycosyl transferase, comprising the following steps: carrying out a reaction on the leuconostoc mesenteroides glucose glycosyl transferase, cane sugar and alcohol in the water containing a buffer solution; and separating and collecting to obtain the salidroside or an analogue product. The enzyme catalytic reaction process disclosed by the invention is finished in a water phase and higher yield, is convenient and simple in operation; the product is easy to separate and purify, and has low environmental pollution and lower production cost; and the glucoside purity is higher after purification; and the process has large industrial application potential, and can satisfy the industrial requirements, such as medicaments and spices and the like.
Owner:ZHENGZHOU UNIVERSITY OF LIGHT INDUSTRY
Ocean protist and method for fermenting and producing high-value-added lipid product
ActiveCN108004149ANotable featuresSignificant positive effectFungiMicroorganism based processesLipid formationBiotechnology
The invention discloses schizochytrium limacinum named as Aurantiochytrium sp, and preserved in China General Microbiological Culture Collection Center (CGMCC) located at No. 3, courtyard 1, the western Beichen road, Chaoyang District of Beijing on Nov. 2nd, 2017, and the preservation number is CGMCC No:14849. The schizochytrium limacinum can be used for preparing DHA, docosapentaenoic acid greaseand squalene. The strain can resist high fermentation temperature, and the fermentation cost can be effectively lowered; the strain can rapidly grow under the condition that the temperature is 33-37DEG C, a large amount of polyunsaturated fatty acids (DHA and docosapentaenoic acid) and squalene grease can be accumulated, the growth speed is high, and the yield of polyunsaturated fatty acid grease is high.
Owner:青岛洪邦生物技术有限公司
Low-temperature activation efficient ammonia decomposition catalyst
PendingCN113181957AShorten crystallization timeReduce stackingFerrierite aluminosilicate zeoliteMolecular sieve catalystsMolecular sieveMetallic Nickel
The invention provides a low-temperature activation efficient ammonia decomposition catalyst and a preparation method thereof, and belongs to the field of ammonia decomposition catalysis. The catalyst is a nickel-containing FER molecular sieve; according to the molecular sieve, a nickel-containing carbon material is taken as a nickel source and a hard template agent, and metal nickel is introduced into the FER molecular sieve in situ; and the content of nickel in the nickel-containing molecular sieve is 0.1-5wt.% in terms of nickel element. the invention also provides a preparation method of the catalyst. The method specifically comprises the following steps of: adding a nickel-containing carbon material, a silicon source, an aluminum source, an organic template agent, inorganic base and a solvent into a reactor according to proportions; continuously stirring the materials to obtain uniform sol, aging the uniform sol; transferring the sol to a hydrothermal reaction kettle for crystallization; performing filtering, washing, drying and roasting on a product which is obtained after the reaction is finished; carrying out hydrogen transformation on an obtained molecular sieve; and finally roasting to obtain the nickel-containing FER molecular sieve. The catalyst disclosed by the invention has the advantages of rich microporous structure, short crystallization time and high catalyst activity and stability; the temperature required by ammonia decomposition is greatly reduced; and the catalyst has huge industrial application potential.
Owner:XIAMEN UNIV
Controllable-optical-property shape-memory polymer film and preparation method thereof
ActiveCN104086791AGood light transmission in visible light bandFlat surfacePolymer scienceOptical property
The invention discloses a controllable-optical-property shape-memory polymer film and a preparation method thereof. Styryl shape-memory polymer solution prepared proportionally is used as the raw material and subjected to a micro photoetching silyl template and spin coating technique to prepare the multiple shape-memory polymer micropattern structure film. The preparation method is simple to operate, and has the advantages of low cost and controllable film thickness; and film surface micro / nano structure can be predesigned according to the application demands, and is applicable to multiple application demands in the field of micro-optics. The styryl shape-memory polymer film has excellent shape memory performance, and can implement the process of quick recovery of the imprinting disappearance on the structural film surface under thermal excitation. By combining the shape-memory characteristic of the material, the method can implement controllable optical properties of the structural film, and has wide application prospects in the fields of tunable micro optical devices, color-control intelligent buildings, display devices, sensors and the like.
Owner:HARBIN INST OF TECH
Method for producing oyster hydrolyzate through microbial fermentation
InactiveCN102326796ATake advantage ofProcess parameter design is reasonableFood preparationAmylaseMicroorganism
The invention discloses a method for preparing an oyster hydrolyzate through microbial fermentation, which is characterized in that a pacific oyster is fermented under the action of Aspergillus oryzae to prepare an oyster hydrolyzate. The preparation process comprises the following steps: taking fresh oyster meat, cleaning, homogenizing, and inoculating with the Aspergillus oryzae for fermentation; and after the fermentation is finished, carrying out filtration sterilization, and concentrating the filtrate to obtain the oyster hydrolyzate. By adopting a liquid fermentation mode, the method has the advantages of reasonable design of process parameters, high operability, low cost and high total nitrogen extraction rate and glycogen extraction rate, avoids the use of finished proteases and amylases, can make full use of oyster resources, and has better industrial application potential.
Owner:HUAIHAI INST OF TECH
Preparation method of NH3-SCR flue gas desulfurization catalyst and catalyst thereof
InactiveCN108816274AImprove water and sulfur resistanceLarge specific surface areaGas treatmentMolecular sieve catalystsNitrateCopper nitrate
The invention relates to the technical field of the flue gas desulfurization catalyst, and specifically relates to a preparation method of an NH3-SCR flue gas desulfurization catalyst and the catalystthereof. The preparation method of the catalyst comprises the following steps: dispersing ZSM-5 zeolite carrier into a composite solution of ferric nitrate and copper nitrate, wherein the mole ratioof Fe atoms to Cu atoms in the composite solution is 1:4, the mass of the Fe element is 10% of the mass of the ZSM-5 carrier, and the dispersing method comprises stirring for 4-6h at 50-60 DEG C by adopting a magnetic stirrer, and ultrasonically oscillating for 40min; discarding supernatant after stratification of the dispersion system, drying a precipitate in an electric constant-temperature blast air oven, grinding the precipitate into powder after drying, then roasting in a muffle furnace, and repeatedly grinding into powder after roasting, thereby obtaining the flue gas desulfurization catalyst. The catalyst prepared with the method disclosed by the invention has a relatively wide active temperature window, and has the advantages of being low in price, environmentally-friendly and thelike.
Owner:HENAN UNIV OF SCI & TECH
Method of in-situ crystallized synthesis of ZSM-5/mordenite compound
ActiveCN103253683AAvoid it happening againLarge specific surface areaMordenite aluminosilicate zeolitePentasil aluminosilicate zeoliteMicrosphereMordenite
The invention provides a method of in-situ crystallized synthesis of ZSM-5 / mordenite compound by using a direct method without a template. The method comprises pre-treating high-temperature-roasted kaolin microspheres by using sodium silicate, adding acid to prepare a reaction mixture, and performing hydrothermal crystallization to obtain in-situ products containing the ZSM-5 / mordenite compound and ex-situ ZSM-5 / mordenite composite molecular sieve. The kaolin microsphere in-situ crystallized ZSM-5 / mordenite composite molecular sieve prepared by the invention is relatively high in relative crystallinity of the ZSM-5 / mordenite, wherein the crystallinity can be adjusted according to requirements, is good in wear resistance performance, and can be used to improve propylene yield in a FCC technology. The co-produced ex-situ ZSM-5 / mordenite composite molecular sieve has a crystallinity of the ZSM-5 being more than 80%, and a crystallinity of the mordenite being more than 70 %.
Owner:PETROCHINA CO LTD
Oxygen-containing polydentate ligand modified copper-based catalyst for acetylene hydrochlorination reaction as well as preparation method and application thereof
ActiveCN112916049AReduce generationHigh activityPreparation by halogen halide additionOrganic-compounds/hydrides/coordination-complexes catalystsActivated carbonPtru catalyst
The invention relates to an oxygen-containing polydentate ligand modified copper-based catalyst for acetylene hydrochlorination reaction as well as a preparation method and application thereof. According to the catalyst, activated carbon serves as a carrier, copper salt serves as a main active component, and the catalytic activity and stability of the catalyst are improved by adding an oxygen-containing polydentate ligand. The oxygen-containing polydentate ligand does not contain other hetero-atoms except oxygen, and can form a stable complex with copper; the copper salt is selected from one or more of copper chloride, copper bromide, copper nitrate and copper sulfate; and the mass ratio of the copper element to the catalyst is 2.5-16%. The copper ions can be firmly combined with the oxygen-containing polydentate ligand, so that the copper ions are prevented from being reduced into inactive zero-valent copper in the catalytic process. In addition, if the two can form a five-membered ring complex after coordination, the electrons of the copper ions can be regulated and controlled to tend to space distribution which is more favorable for catalysis.
Owner:NANKAI UNIV +1
A kind of deep eutectic solvent for extraction and denitrification of fuel oil and its application method
ActiveCN109181747BIncrease polarityHigh yieldOrganic compound preparationHydrocarbon oils refiningNitrogen removalPolythylene glycol
The invention relates to a eutectic solvent for fuel extraction and denitrification and a use method thereof. The eutectic solvent is prepared by mixing poly(ethylene glycol)bis(carboxymethyl) ether having a molecular weight of 250 and quaternary ammonium salt, the eutectic solvent and fuel are directly mixed after extraction and denitrification and subjected to standing, and liquid separation iscarried out to obtain a denitrified oil product and a nitrogen-rich extract. When the eutectic solvent is used for extracting and denitrifying the fuel, the nitrogen removal rate can reach 50% to 99.9%, the nitrogen distribution coefficient is 8.0 to 110, the aromatics selectivity is 300 and more, and the oil yield is greater than 95 %.
Owner:WUHAN INSTITUTE OF TECHNOLOGY
Eutectic solvent for fuel extraction and denitrification and use method thereof
ActiveCN109181747AIncrease polarityHigh yieldHydrocarbon oils refiningHydrocarbon oils treatmentNitrogen removalPolyethylene glycol
The invention relates to a eutectic solvent for fuel extraction and denitrification and a use method thereof. The eutectic solvent is prepared by mixing poly(ethylene glycol)bis(carboxymethyl) ether having a molecular weight of 250 and quaternary ammonium salt, the eutectic solvent and fuel are directly mixed after extraction and denitrification and subjected to standing, and liquid separation iscarried out to obtain a denitrified oil product and a nitrogen-rich extract. When the eutectic solvent is used for extracting and denitrifying the fuel, the nitrogen removal rate can reach 50% to 99.9%, the nitrogen distribution coefficient is 8.0 to 110, the aromatics selectivity is 300 and more, and the oil yield is greater than 95 %.
Owner:WUHAN INSTITUTE OF TECHNOLOGY
Ethanol-tolerant, high-temperature-tolerant and high-permeability-tolerant multi-tolerance fermentation strain and construction method
ActiveCN112195112AImprove high temperature resistanceEthanol is well toleratedFungiMutant preparationMutant strainBiotechnology
Owner:CHINA PETROLEUM & CHEM CORP +2
In-situ synthesized Pt/MnO2@Mn3O4 catalyst for catalytic combustion of VOCs (Volatile Organic Compounds) as well as preparation method and application of in-situ synthesized Pt/MnO2@Mn3O4 catalyst
ActiveCN108816225AAchieve complete catalytic combustionImprove stabilityCatalyst activation/preparationIncinerator apparatusPotassium permanganateReducing agent
The invention belongs to the technical field of environment catalytic purification and provides an in-situ synthesized Pt / MnO2@Mn3O4 catalyst for catalytic combustion of VOCs (Volatile Organic Compounds) as well as a preparation method and application of the in-situ synthesized Pt / MnO2@Mn3O4 catalyst. The preparation method comprises the following steps: taking potassium permanganate and oxalic acid as raw materials and preparing MnO2 by utilizing a hydrothermal method; then taking MnO2 as an initial carrier and utilizing sodium borohydride as a reducing agent; carrying out liquid-phase reduction on H2PtC16 under a room-temperature condition, and carrying out structure regulation and control on the MnO2 carrier; carrying out one-step reduction to obtain an in-situ synthesized mixed-phase MnO2@Mn3O4 catalyst carrier loading type Pt-based catalyst, wherein the content of Pt is 0.2 to 0.5 weight percent. The preparation method provided by the invention has a simple preparation process; the dispersed loading of active components and the optimization of a carrier structure can be realized in one step. The catalyst provided by the invention is applied to the catalytic combustion of the VOCs including toluene, xylol and the like and has relatively good low-temperature activity and stability; no other waste gas is generated.
Owner:DALIAN UNIV OF TECH
Industrial production method of copper nanoparticle
ActiveCN102407344BThe process steps are simpleShort processMaterial nanotechnologyReduction treatmentHydrazine compound
Owner:西安坤德新型材料有限公司
Chlorine-containing organic waste gas catalytic combustion catalyst and preparation method thereof
PendingCN110787797AStrong resistance to chlorine poisoningUnique cage structureIncinerator apparatusMetal/metal-oxides/metal-hydroxide catalystsAir atmospherePtru catalyst
The invention discloses a chlorine-containing organic waste gas catalytic combustion catalyst and a preparation method thereof, belongs to the field of chemical preparation, and solves the problem that a conventional catalyst is easy to inactivate due to Cl poisoning. According to the chlorine-containing organic waste gas catalytic combustion catalyst, a composite oxide dodecacalcium heptaaluminate is used as a carrier, PtO2 is used as a precious metal active center, and the PtO2 accounts for 0.1-0.3% of the total mass of the catalyst. The preparation method comprises the steps: mixing a chloroplatinic acid solution with a sodium hydroxide solution, adjusting the pH value to be greater than or equal to 11, and adding deionized water to prepare a soluble Pt precursor solution; adding a composite oxide dodecacalcium heptaaluminate carrier, and stirring; adding nitric acid or acetic acid, and adjusting the pH value of the system to 3-4; calcining, crushing and granulating in an air atmosphere to obtain the chlorine-containing organic waste gas catalytic combustion catalyst. The catalyst provided by the invention has extremely high Cl poisoning resistance and good heat-resistant stability. According to the method, the problem of PtO2 loading is solved, and the process is simple.
Owner:JINCHUAN GROUP LIMITED +1
Method for constructing L-ergothionine producing strains
InactiveCN111363760AFood source safeGood potential for industrial applicationsFungiMicroorganism based processesBiotechnologyRhodotorula
The invention discloses rhodotorula toruloides engineering bacteria and a construction method. The rhodotorula toruloides engineering bacteria can express exogenous egt1 enzymes, so as to improve thecapacity for producing L-ergothionine, and after fermentation, the yield of the L-ergothionine is close to 5g / L. The method has industrial application prospects.
Owner:ZHEJIANG HUARUI BIOTECHNOLOGY CO LTD
Multi-tolerance saccharomyces cerevisiae strain and construction method and application thereof
The invention discloses a multi-tolerance saccharomyces cerevisiae strain and a construction method and application thereof. The saccharomyces cerevisiae strain is preserved in the China general microbiological culture Collection center, the preservation name is SEB17, and the preservation number is CGMCC NO. 22587. The construction method comprises the steps that a strain SEB14 is adopted as an original strain, a promoter of a functional gene ENA5 of the strain SEB14 is replaced with a promoter of TEF1, and the strain SEB17 is constructed. The strain SEB17 has the multi-tolerance, has the advantage of being resistant to various environmental stresses such as high ethanol, high temperature, high sugar and high salt, and can be suitable for material fermentation under various environmental stresses.
Owner:SICHUAN UNIV
Fluoride-free durable super-hydrophobic cotton cloth and preparation method and application thereof
ActiveCN112080938AStable super hydrophobic performanceIncrease sulfhydrylStain/soil resistant fibresLiquid repellent fibresFiberPolymer science
The invention discloses fluoride-free durable super-hydrophobic cotton cloth and a preparation method and application thereof, and belongs to the field of super-hydrophobic materials. The preparationmethod comprises the following steps that hydrolysis reaction is carried out on 3-methacryloxypropyltrimethoxy silane and silicon dioxide to obtain modified silicon dioxide; secondly, cotton cloth fibers are modified by using 3-mercaptopropyltriethoxysilane, so that mercapto groups on the surfaces of the fibers are functionalized; and finally, under the action of a photoinitiator and illuminationof a mercury lamp, sulfydryl-alkene click chemical reaction is carried out on sulfhydrylation cotton cloth, methacryloyloxy polydimethylsiloxane and the modified silicon dioxide to prepare the super-hydrophobic cotton cloth. The super-hydrophobic cotton cloth prepared by the method has self-cleaning property and an oil-water separation function. Raw materials are cheap, easy to obtain and environmentally friendly, and the prepared material is stable in hydrophobicity and has good wear resistance. Meanwhile, the preparation method is simple, mild in preparation condition and convenient for large-scale production, and has huge industrial application potential.
Owner:EAST CHINA NORMAL UNIV
4D intelligent aerogel and preparation method thereof
ActiveCN109293979ALow densityHigh porosityAdditive manufacturing apparatusArtificial enzymeNew energy
The invention relates to a 4D intelligent aerogel and a preparation method thereof. The preparation method is characterized by regulating the shape memory property and regulating the microstructure, the density and the hydrophilicity. The 4D intelligent aerogel is prepared by a one-step crosslinking vacuum freeze-drying process, and the technical problems of complexness and poor controllability ofexisting intelligent aerogel preparation methods are solved. The preparation method has the advantages of simple preparation process, low cost, good controllability, strong universality, and easinessin modification and compounding of a material. The 4D aerogel has the advantages of strong controllability, good structural designability, good density and hydrophilicity controllability and good mechanical properties, can be used in fields of marine development, intelligent drugs, environmental protection, porous electrodes, intelligent adsorption, intelligent artificial enzymes, intelligent sound absorption, new energy development, intelligent protection, building materials, lightweight structural materials, thermal insulation materials, flame retardant materials, shock absorbing materials,reinforced framework materials, energy storage devices and aerospace, ad provides a way for studying multifunctional materials.
Owner:HAINAN UNIVERSITY +1
Glucose transporter and application thereof to improving organic acid production
ActiveCN112250740AIncrease production capacityIncrease acid productionFungiAntibody mimetics/scaffoldsOrganic acidGlucose Transport Protein
The invention discloses a protein with a glucose transport function in filamentous fungi and an application of the protein to improving organic acid production, and belongs to the field of bioengineering and genetic engineering. Besides, the invention also discloses a construction method of the engineering strain and a method for producing organic acid, especially citric acid, by using the strain.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
Method for increasing yield of transglutaminase produced by fermentation
ActiveCN112695018AReduced zymogen concentrationReduce osmotic pressureBacteriaMicroorganism based processesBiotechnologyStreptoverticillium mobaraense
The invention discloses a method for increasing yield of transglutaminase produced by fermentation. According to the method, Streptoverticillium mobaraense DSM 40587 is adopted as an experimental strain, slant spores are subjected to seed culture and then inoculated into a fermentation medium added with soybean polysaccharide to be fermented to prepare transglutaminase (MTG), and results show that expression level of the transglutaminase is remarkably increased. Wherein addition of 6 g / L of soybean polysaccharide shows the best effect. The method has advantages that the soybean polysaccharide used is non-toxic, harmless, low in cost, convenient to operate, and can improve efficiency of industrial production of transglutaminase and reduce economic costs. Under the same experimental conditions, the addition of soybean polysaccharides can advance enzyme production time by 27.6% or more, significantly shortening fermentation time.
Owner:EAST CHINA NORMAL UNIVERSITY
Quenching process applied to high-hardness engineering machinery
ActiveCN106041030AExtended service lifeRealize automatic deliveryFurnace typesMoulding flasksQuenchingMaterials science
The invention discloses a quenching process applied to high-hardness engineering machinery and belongs to the technical field of machining methods for excavator parts. The quenching process comprises the following steps: (1) manufacturing of a sand box which comprises an upper sand box body and a lower sand box body: forming a bucket tooth cavity in the lower sand box body, arranging electric heating tubes around the bucket tooth cavity, and arranging a mold-releasing liquid guiding mechanism with an automatic daubing function; (2) smelting; (3) casting; and (4) forming: ensuring that a rotating table stop rotating after condensation forming, opening the sand box and taking out a mold. By adoption of the quenching process disclosed by the invention, the technical problem that the top and the root of a conventional bucket tooth are made of a uniform material, and thus the conventional bucket tooth is short in service life can be solved; the quenching process is widely applied to the production of excavator bucket teeth; and meanwhile, the mold-releasing liquid is more convenient to daub, and the quenching is further facilitated.
Owner:宁波吉威熔模铸造有限公司
Method for constructing L-ergothionine producing strain
ActiveCN111534535AFood source safeStable genetic traitsFungiMicroorganism based processesBiotechnologyMicrobiology
The invention discloses a rhodotorula glutinis engineering bacterium and a construction method thereof. The rhodotorula glutinis engineering bacterium can express exogenous egt1 enzyme so as to improve the capacity of producing L-ergothionine, the yield of the fermented L-ergothionine is close to 1.5 g / L, and the engineering bacterium is stable in inheritable character and has an industrial application prospect.
Owner:ZHEJIANG HUARUI BIOTECHNOLOGY CO LTD
Mutant of D-psicose 3-epimerase and application of mutant
ActiveCN113373135AImprove thermal stabilityExtended half-lifeRacemaces/epimerasesIsomerasesWild typePsicose
The invention relates to a mutant of D-psicose 3-epimerase and application of the mutant. A series of mutants with improved fermentation enzyme activity are obtained by performing directed evolution on wild type DPEase from Clostridium cellulyticum. The fermentation enzyme activity of the DPEase mutant H56Q is 1.61 times that of the wild type; and the half-life period of the mutant H56Q at 55 DEG C is prolonged by 87.5% compared with that of the wild type, the thermal stability is improved, and the good industrial application potential is achieved.
Owner:JIANGNAN UNIV
Synthesis method of cyclohexane dimethanol, catalyst and application thereof
ActiveCN107986943AWill not emitRaw materials are easy to getOrganic compound preparationOrganic-compounds/hydrides/coordination-complexes catalystsSynthesis methodsCyclohexene
The invention provides a synthesis method of cyclohexane dimethanol. The synthesis method comprises the following step: under the action of a catalyst, performing a hydroformylation reaction and a hydrogenation reaction on cyclohexene formaldehyde to obtain the cyclohexane dimethanol, wherein the catalyst comprises a cobalt compound and an organic tricyclohexylphosphine ligand; the cobalt compoundis preferably a hydrogenated polycarbonyl compound of cobalt. By adopting the synthesis method of the cyclohexane dimethanol, provided by the invention, the use of terephthalic acid and the like is avoided, the synthesis technology is simple, and the cost is low.
Owner:WANHUA CHEM GRP CO LTD
Method of in-situ crystallized synthesis of ZSM-5/mordenite compound
ActiveCN103253683BAvoid it happening againLarge specific surface areaMordenite aluminosilicate zeolitePentasil aluminosilicate zeoliteMolecular sieveMicrosphere
Owner:PETROCHINA CO LTD
Method for producing nuclease P1 through large-scale liquid submerged fermentation
InactiveCN103820418ALow priceShort fermentation timeHydrolasesMicroorganism based processesSubmerged fermentationNuclease
The invention provides a technology for producing nuclease by adopting penicillium citrinum strain to perform large-scale liquid submerged fermentation. The technology comprises the following steps: firstly, performing slant culture of the strain, secondly, performing strain culture on a shaker, thirdly, performing strain enlarging culture in a fermentation tank with the volume of 500 L, and fourthly, performing large-scale production in the fermentation tank. By the adoption of the fermentation technology, the fermentation production time is only 50 to 70h in a 10-ton fermentation tank, and the activity of nuclease in fermentation liquor reaches 1,180 U / ml. The technology is relatively low in cost; compared with the activity (300 U / ml) of malt sprout extracted enzyme stock solution, the enzyme activity is greatly improved.
Owner:曾化伟
A method for constructing ergothioneine-producing bacteria
ActiveCN111534535BFood source safeStable genetic traitsFungiMicroorganism based processesBiotechnologyGenetic traits
The invention discloses a rhodotorula torula engineering bacterium and a construction method thereof. The torula rhodotorula engineering bacterium can express exogenous egt1 enzyme, thereby improving the ability to produce ergothioneine, and the yield of ergothioneine after fermentation is close to 1.5g / L , and the genetic properties of the engineering bacteria are stable, and have industrial application prospects.
Owner:ZHEJIANG HUARUI BIOTECHNOLOGY CO LTD
Denitration catalyst and preparation method thereof, and waste gas denitration method
ActiveCN113368865AImprove dispersionBalances Acidity and OxidationDispersed particle separationMetal/metal-oxides/metal-hydroxide catalystsManganese oxideExhaust fumes
The invention provides a denitration catalyst and a preparation method thereof, and a waste gas denitration method, belonging to the field of waste gas treatment. The denitration catalyst comprises a core layer, a middle layer and a shell layer, wherein the core layer comprises titanium nanotubes, the middle layer comprises manganese oxide, and the shell layer comprises iron oxide. The preparation method of the denitration catalyst comprises the following steps: subjecting a manganese oxide precursor to reacting with the titanium nanotubes to obtain the titanium nanotubes loaded with the manganese oxide, and then modifying the titanium nanotubes loaded with the manganese oxide by using an iron source to obtain the denitration catalyst. According to the waste gas denitration method, waste gas denitration is carried out by using the denitration catalyst. The denitration catalyst provided by the invention has higher stability, activity, selectivity and sulfur resistance; raw materials used in the preparation method are environment-friendly and easily available, operation is simple and controllable, and expanded production is facilitated; and when the denitration catalyst is used for denitration treatment of waste gas, a denitration rate is high, selectivity is good, and stability is high.
Owner:SHANXI INST OF COAL CHEM CHINESE ACAD OF SCI
Glucose transporters and their use in enhancing organic acid production
ActiveCN112250740BIncrease production capacityIncrease acid productionFungiAntibody mimetics/scaffoldsBiotechnologyOrganic acid
The invention discloses a protein with glucose transport function in filamentous fungi and its application in improving the production of organic acids, belonging to the fields of bioengineering and genetic engineering. At the same time, the invention also discloses a method for constructing an engineering strain and a method for producing organic acid, especially citric acid, using the strain.
Owner:TIANJIN INST OF IND BIOTECH CHINESE ACADEMY OF SCI
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