87results about How to "Free from degradation" patented technology

The invention provides EV71virus-like particles as well as preparation method and application of the EV71virus-like particles. The method comprises the following steps of: connecting a P1 protein gene and 3CD protease gene of an EV71 virus with predicated mean vote (PMV) plasmid to build PMV-P1-3CD recombinant expression plasmid; turning into hansenula polymorpha AU-0501 expression bacterial strain to obtain an AU-PMV-P1-3CD recombinant expression bacterial strain; fermenting and culturing the recombinant expression bacteria strain and performing inducible expression on EV71 virus-like particle proteins by using methanol; centrifugally gathering thallus to perform homogenate crushing at high pressure; and purifying the supernate through ion-exchange column chromatography, hydrophobic chromatography and sieve chromatography to obtain the EV71virus-like particles. EV71virus-like particle vaccines provided by the invention has high immunogenicity, security, immunological characteristics and biological activity; and the EV71virus-like particles can be prepared in a large scale and purified and used for preparing vaccines for preventing EV71 infection, and has good economic value and application prospect.

The invention discloses a method for preparing regenerative silk fibroin. The method includes adding a calcium alcohol solution into degumming silks, dissolving the degumming silks through water bath, centrifugally removing suspended impurities, and performing dialysis and drying to obtain the regenerative silk fibroin. The preparation method is simple, the dissolvability of the silk fibroin is good, the structure of the prepared regenerative silk fibroin is a beta-folding sheet layer, the irregular curl structure is decreased, the regenerative silk fibroin can be used as a carrier for immobilizing enzyme, and the enzyme can be immobilized on the regenerative silk fibroin, so that the enzyme can be prevented from being degraded by degeneration enzymes in an organism, and further the stability and the half-life period of the enzyme are improved.

A semiconductor integrated circuit has a first substrate of a first polarity to which a first substrate potential is given, a second substrate of the first polarity to which a second substrate potential different from the first substrate potential is given, and a third substrate of a second polarity different from the first polarity. The first substrate is insulated from a power source or ground to which a source of a MOSFET formed on the substrate is connected. The third substrate is disposed between the first and second substrates in adjacent relation to the first and second substrates. A circuit element is formed on the third substrate.